LOCUS       sequence-41594-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag-YAP-S127A/G385V
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctcgtttag tgaaccgtca gaattgatct accatggact acaaagacga tgacgacaag
       61 cttgcggccg cgaattcaag cttagccacc atggactaca aagacgatga cgataaagca
      121 aggctcgaat cggtacctaa ggatcccggg cagcagccgc cgcctcaacc ggccccccag
      181 ggccaagggc agccgccttc gcagcccccg caggggcagg gcccgccgtc cggacccggg
      241 caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac cccccgccgg gcatcagatc
      301 gtgcacgtcc gcggggactc ggagaccgac ctggaggcgc tcttcaacgc cgtcatgaac
      361 cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc tccggaagct gcccgactcc
      421 ttcttcaagc cgccggagcc caaatcccac tcccgacagg ccagtactga tgcaggcact
      481 gcaggagccc tgactccaca gcatgttcga gctcatgcct ctccagcttc tctgcagttg
      541 ggagctgttt ctcctgggac actgaccccc actggagtag tctctggccc agcagctaca
      601 cccacagctc agcatcttcg acagtcttct tttgagatac ctgatgatgt acctctgcca
      661 gcaggttggg agatggcaaa gacatcttct ggtcagagat acttcttaaa tcacatcgat
      721 cagacaacaa catgg
//
