LOCUS       sequence-415940-       13783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible plasmid for TurboID-based proximity labelling 
            experiment in planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Tan H, Zhou Y, Dinius E, Lozano-Duran R
  TITLE     The Ti-TAN plasmid toolbox for TurboID-based proximity labeling 
            assays in Nicotiana benthamiana.
  JOURNAL   J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610.
  PUBMED    38294100
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610."
COMMENT     Sequence Label: pGW-GOI-TurboID-GFP
FEATURES             Location/Qualifiers
     source          1..13783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1303
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1340..1345,1346..1363,1364..1370)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1424..2104
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1490..1509)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2424..2729
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2608..2627
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2770..2894)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2914..2940
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2944..2970
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2974..3000
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3007..3963
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             join(3964..3966,3967..4680)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4267..4286)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4614..4635
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4705..4957
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4990..5006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4990..5006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5003..5025)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5014..5030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5038..5044,5045..5062,5063..5068))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5083..5104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5140..5392)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5543..6568)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6620..6799)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7305..7329
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7850..8641
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8887..9475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9376..9395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9629..9646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9661..9801
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9727..9746)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9887..9909
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10145..10339
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10405..11478)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11907..12536)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgagaggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca ggcggggcgt aatctagagg atccggctta
     2101 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2161 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2221 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2281 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2341 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2401 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2461 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2521 tgatccccct ggccagtgca cgtctgctgt cagataaagt cccccgtgaa ctttacccgg
     2581 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     2641 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2701 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2761 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     2821 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2881 ttgtacaaag tggttcgatc tagaggatcc atgtacccgt atgatgttcc ggattacgct
     2941 ggctatccct acgacgtgcc cgactatgcc gggtacccct atgacgtccc agactacgca
     3001 gctagcaaag acaatactgt gcctctgaag ctgatcgctc tcctggctaa tggcgagttc
     3061 catagtggcg aacagctggg agaaaccctg ggcatgtcca gggccgctat caacaagcac
     3121 attcagactc tgcgcgactg gggcgtggac gtgttcaccg tgcccggaaa gggctactct
     3181 ctgcccgagc ctatcccgct gctgaacgct aaacagattc tgggacagct ggacggcggg
     3241 agcgtggcag tcctgcctgt ggtagactcc accaatcagt acctgctgga tcgaatcggc
     3301 gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag aggaagcaga
     3361 gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt ttggcgcctg
     3421 aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg
     3481 ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct gtatctgcag
     3541 gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga tgctgcacag
     3601 atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag cgtggtcaat
     3661 cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac tctggccgct
     3721 acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg cctggctcca
     3781 tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa gctgatcatt
     3841 ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc cctgctgctg
     3901 gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg gtctgccgaa
     3961 aagatgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4021 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4081 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4141 ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4201 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4261 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4321 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4381 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4441 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4501 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4561 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4621 ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct gtacaagtaa
     4681 agcggcccga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
     4741 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     4801 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     4861 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     4921 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattggttcc ggaaccaatt
     4981 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5041 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5101 attaggctga attaggcgcg cctatttcta ttaattcccg atctagtaac atagatgaca
     5161 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     5221 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     5281 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     5341 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat
     5401 tcgggggatc gatcccgcga actgtggacg agaactgtgc caccaagcgt aaggccgttc
     5461 tctcgcattt gccttgctag gctcgcgcga gttgctggct gaggcgttct cgaaatcagc
     5521 tcttgttcgg tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt
     5581 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
     5641 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
     5701 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
     5761 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
     5821 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
     5881 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
     5941 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
     6001 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     6061 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     6121 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     6181 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     6241 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     6301 aataggtcag gctctcgctg aatgccccaa tgtcaagcac ttccggaatc gggagcgcgg
     6361 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     6421 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     6481 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     6541 acgtcgcggt gagttcaggc tttttcatat cttattgccc cccgggatca gatctggatt
     6601 gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc
     6661 atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa
     6721 taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc
     6781 tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg
     6841 cgggggtcat aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc
     6901 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
     6961 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
     7021 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
     7081 tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
     7141 tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag
     7201 ctgatccgtc gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt
     7261 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     7321 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     7381 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     7441 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     7501 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     7561 gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     7621 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     7681 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     7741 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     7801 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
     7861 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     7921 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     7981 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     8041 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     8101 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     8161 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     8221 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     8281 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     8341 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     8401 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     8461 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     8521 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     8581 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     8641 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     8701 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     8761 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
     8821 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8881 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9001 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     9061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9541 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9601 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9661 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     9721 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     9781 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     9841 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     9901 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
     9961 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag
    10021 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
    10081 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga
    10141 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
    10201 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
    10261 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
    10321 aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat
    10381 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
    10441 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
    10501 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
    10561 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
    10621 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
    10681 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
    10741 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
    10801 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
    10861 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
    10921 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
    10981 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
    11041 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
    11101 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
    11161 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
    11221 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
    11281 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
    11341 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
    11401 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
    11461 tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
    11521 cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
    11581 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
    11641 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
    11701 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
    11761 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
    11821 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
    11881 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
    11941 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
    12001 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
    12061 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
    12121 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
    12181 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
    12241 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
    12301 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
    12361 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
    12421 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
    12481 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
    12541 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
    12601 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
    12661 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
    12721 gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
    12781 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
    12841 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
    12901 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
    12961 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
    13021 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
    13081 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
    13141 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
    13201 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
    13261 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
    13321 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
    13381 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
    13441 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
    13501 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
    13561 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
    13621 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa
    13681 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc
    13741 ctgtggttgg catgcacata caaatggacg aacggataaa cct
//
