LOCUS       sequence-41595-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     Charles Vinson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMV500 A-ATF2
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             116..148
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(391..409)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(392..409)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(429..446)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    435..661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(794..813)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ccaccatgga ctacaaggac gacgatgaca agcatatggc
      121 tagcatgact ggtggacagc aaatgggtcg ggatcctgac ctggaacaac gtgctgagga
      181 actggcccgt gaaaacgaag agctggaaaa agaggccgaa gagctggagc aggaactggc
      241 agaactcgag aagaaagctg aagacttgag ttcattaaat ggtcagctgc agagtgaagt
      301 caccctgctg agaaatgaag tggcacagct gaaacagctt cttctggctc ataaagattg
      361 cccttaattc aagcttctag agggccctat tctatagtgt cacctaaatg ctagagctcg
      421 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      481 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      541 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg gggtgggggt ggggcaggac
      601 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      661 gcttctgagg cggaaagaac cagctggggc tcgagggggg atccccacgc gccctgtagc
      721 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      781 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      841 ccccgtcaag ctctaaatc
//
