LOCUS       sequence-41597-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     Charles Vinson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMV500 A-C/EBP-beta
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..109
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             113..145
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(406..424)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(407..424)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(444..461)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    450..676
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(809..828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctcca ccatggacta caaggacgac gatgacaagc atatggctag
      121 catgactggt ggacagcaaa tgggtcggga tcctgacctg gaacaacgtg ctgaggaact
      181 ggcccgtgaa aacgaagagc tggaaaaaga ggccgaagag ctggagcagg aaaacgctga
      241 actcgagcac aaggtgctgg agctgacggc ggagaacgag cggctgcaga agaaggtgga
      301 gcagctgtcg cgagagctca gcacgctgcg gaacttgttc aagcagctgc ccgagccgct
      361 gctggcctcg gcgggccact gctagaagct tctagagggc cctattctat agtgtcacct
      421 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
      481 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
      541 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tgggggggtg
      601 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
      661 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag gggggatccc
      721 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      781 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      841 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctt
//
