LOCUS       sequence-41599-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Ahn S, Olive M, Aggarwal S, Krylov D, Ginty DD, Vinson C
  TITLE     A dominant-negative inhibitor of CREB reveals that it is a general 
            mediator of stimulus-dependent transcription of c-fos.
  JOURNAL   Mol Cell Biol. 1998 Feb . 18(2):967-77.
  PUBMED    9447994
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Feb . 18(2):967-77."
COMMENT     Sequence Label: CMV500 A-CREB
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..109
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             113..145
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(361..379)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(362..379)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(399..416)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    405..629
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctcca ccatggacta caaggacgac gatgacaagc atatggctag
      121 catgactggt ggacagcaaa tgggtcggga tcctgacctg gaacaacgtg ctgaggaact
      181 ggcccgtgaa aacgaagagc tggaaaaaga ggccgaagag ctggagcagg aactggcaga
      241 actcgagaac agagtggcag tgcttgaaaa ccaaaacaaa acattgattg aggagctaaa
      301 agcacttaag gacctttact gccacaagtc agattaattc aagcttctag agggccctat
      361 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
      421 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      481 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      541 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
      601 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
      661 gaggggggat ccccacgcgc cctgtag
//
