LOCUS       sequence-41607-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag-YAP-S127/D383A
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagagctcgt ttagtgaccg tcagaattga tctaccatgg actacaaaga cgatgacgac
       61 aagcttgcgg ccgcgaattc aagcttagcc accatggact acaaagacga tgacgataaa
      121 gcaaggctcg aatcggtacc taaggatccc gggcagcagc cgccgcctca accggccccc
      181 cagggccaag ggcagccgcc ttcgcagccc ccgcaggggc agggcccgcc gtccggaccc
      241 gggcaaccgg cacccgcggc gacccaggcg gcgccgcagg caccccccgc cgggcatcag
      301 atcgtgcacg tccgcgggga ctcggagacc gacctggagg cgctcttcaa cgccgtcatg
      361 aaccccaaga cggccaacgt gccccagacc gtgcccatga ggctccggaa gctgcccgac
      421 tccttcttca agccgccgga gcccaaatcc cactcccgac aggccagtac tgatgcaggc
      481 actgcaggag ccctgactcc acagcatgtt cgagctcatg cctctccagc ttctctgcag
      541 ttgggagctg tttctcctgg gacactgacc cccactggag tagtctctgg cccagcagct
      601 acacccacag ctcagcatct tcgacagtct tcttttgaga tacctgatga tgtacctctg
      661 ccagcaggtt gggagatggc aaagacatct tctggtcaga gatacttctt aaatcacatc
      721 gatcagacaa caacatggca ggaccccagg aaggccatgc tgtcccagat gaacgtcaca
      781 gcccccacca gtccaccagt gcagcagaat atgatgaact cggcttcagg tcctcttcct
      841 gatggatggg aacaagccat gactcaggat ggagaaattt actatataaa cc
//
