LOCUS       sequence-41610-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag-YAP-S127/387A
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..54
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagtgaacc gtcagaattg atctaccatg gactacaaag acgatgacga caagcttgcg
       61 gccgcgaatt caagcttagc caccatggac tacaaagacg atgacgataa agcaaggctc
      121 gaatcggtac ctaaggatcc cgggcagcag ccgccgcctc aaccggcccc ccagggccaa
      181 gggcagccgc cttcgcagcc cccgcagggg cagggcccgc cgtccggacc cgggcaaccg
      241 gcacccgcgg cgacccaggc ggcgccgcag gcaccccccg ccgggcatca gatcgtgcac
      301 gtccgcgggg actcggagac cgacctggag gcgctcttca acgccgtcat gaaccccaag
      361 acggccaacg tgccccagac cgtgcccatg aggctccgga agctgcccga ctccttcttc
      421 aagccgccgg agcccaaatc ccactcccga caggccagta ctgatgcagg cactgcagga
      481 gccctgactc cacagcatgt tcgagctcat gcctctccag cttctctgca gttgggagct
      541 gtttctcctg ggacactgac ccccactgga gtagtctctg gcccagcagc tacacccaca
      601 gctcagcatc ttcgacagtc ttcttttgag atacctgatg atgtacctct gccagcaggt
      661 tgggagatgg caaagacatc ttctggtcag agatacttct taaatcacat cgatcagaca
      721 acaacatggc aggaccccag gaaggccatg ctgtcccaga tgaacgtcac agcccccacc
      781 agtccaccag tgcagcagaa tatgatgaac tcggcttcag gtcctcttcc tgatggatgg
      841 gaacaagcca tgac
//
