LOCUS       sequence-41614-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag-YAP-4SA(S61)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..58
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcgtttagt gaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
       61 tgcggccgcg aattcaagct tagccaccat ggactacaaa gacgatgacg ataaagcaag
      121 gctcgaatcg gtacctaagg atcccgggca gcagccgccg cctcaaccgg ccccccaggg
      181 ccaagggcag ccgccttcgc agcccccgca ggggcagggc ccgccgtccg gacccgggca
      241 accggcaccc gcggcgaccc aggcggcgcc gcaggcaccc cccgccgggc atcagatcgt
      301 gcacgtccgc ggggactcgg agaccgacct ggaggcgctc ttcaacgccg tcatgaaccc
      361 caagacggcc aacgtgcccc agaccgtgcc catgaggctc cggaagctgc ccgactcctt
      421 cttcaagccg ccggagccca aatcccactc ccgacaggcc gctactgatg caggcactgc
      481 aggagccctg actccacagc atgttcgagc tcatgccgct ccagctgctc tgcagttggg
      541 agctgtttct cctgggacac tgacccccac tggagtagtc tctggcccag cagctacacc
      601 cacagctcag catcttcgac aggctgcttt tgagatacct gatgatgtac ctctgccagc
      661 aggttgggag atggcaaaga catcttctgg tcagagatac ttcttaaatc acatcgatca
      721 gacaacaaca tggcaggacc ccaggaaggc catgctgtcc cagatgaacg tcacagcccc
      781 caccagtcca ccagtgcagc agaatatgat gaactcggct tc
//
