LOCUS       sequence-416169-       15779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ROSA26 targeting vector for the SAE sensor for ERK1/2 signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15779)
  AUTHORS   Dinsmore CJ, Soriano P
  TITLE     Conditional fluorescent mouse translocation reporters for ERK1/2 and
            AKT signaling.
  JOURNAL   Dev Biol. 2023 Sep 1:S0012-1606(23)00146-X. doi: 
            10.1016/j.ydbio.2023.08.007.
  PUBMED    37660778
REFERENCE   2  (bases 1 to 15779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2023 Sep 1:S0012-1606(23)00146-X. doi: 10.1016/j.ydbio.2023.08.007."
COMMENT     Sequence Label: pROSA26-SAE
FEATURES             Location/Qualifiers
     source          1..15779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    136..597
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    745..879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    999..1133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1253..1387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1416..1449
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1507..1884
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     CDS             1903..2610
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     CDS             2620..2676
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(2974..2995)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3582..3603
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3621..4209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3674..3694)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4092..4103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4212..4228)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4236..4347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4448..8789
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        8839..9338
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             9365..9937
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          10014..10079
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    10153..10377
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10439..10459)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10439..10457)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10478..10494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10478..10494)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10491..10513)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10502..10518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10526..10532,10533..10550,10551..10556))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10571..10592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10709..10726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10880..11468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10960..10979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11639..12430,12431..12499))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12262..12281
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12500..12604)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12631..13086
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12718..12737)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12928..12949
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13212..13234
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13226..13243
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13227..13243
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13253..13272
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13253..13271
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    13290..14371
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(14373..14438,14439..14476)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     protein_bind    14526..14559
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        14589..15088
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             join(15102..15779,1..126)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15165..15184)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     join(15775..15779,1..15)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
       61 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
      121 ttctgagggg atccgctgta agtctgcaga aattgatgat ctattaaaca ataaagatgt
      181 ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct
      241 acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg
      301 cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat
      361 cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta
      421 tagatctata gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct
      481 aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc
      541 tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaagct
      601 tgcagatctg cgactctaga ggatctgcga ctctagagga tcataatcag ccataccaca
      661 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      721 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      841 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gcgactctag aggatcataa
      901 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      961 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     1021 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgcgact
     1141 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     1201 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     1261 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1321 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1381 ctggatcccc atcaagctga tccggaaccc ttaatataac ttcgtataat gtatgctata
     1441 cgaagttatt aggtccctcg acctgcagcc caagctagca gacccaagct ggctagcacc
     1501 gccaccatgc ctgaaccctc taagtctgct ccagccccta aaaagggttc taagaaggct
     1561 atcactaagg cgcagaagaa ggatggtaag aagcgtaagc gcagccgcaa ggagagctat
     1621 tctatctatg tgtacaaggt tctgaagcag gtccaccccg acaccggcat ctcatccaag
     1681 gccatgggga tcatgaactc cttcgtcaac gacatcttcg agcgcatcgc gggcgaggct
     1741 tctcgcctgg ctcactacaa taagcgctcg accatcacct ccagggagat tcagacggct
     1801 gtgcgcctgc tgctgcctgg ggagctggct aagcatgctg tgtccgaggg cactaaggca
     1861 gttaccaagt acactagctc taaggatcca ccggtcgcca ccgtgtctaa gggcgaagag
     1921 ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa
     1981 ttcaaatgca caggtgaagg agaaggcaga ccgtacgagg gagtgcaaac catgaggatc
     2041 aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg
     2101 tatggcagcc gtacctttat caagtacccg gccgacatcc ctgatttctt taaacagtcc
     2161 tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc
     2221 gtcacgcagg acaccagcct tgaggatggc gagctcgtct acaacgtcaa ggtcagaggg
     2281 gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat
     2341 acagagatga tgtatccagc agatggtggt ctgagaggat acactgacat cgcactgaaa
     2401 gttgatggtg gtggccatct gcactgcaac ttcgtgacaa cttacaggtc aaaaaagacc
     2461 gtcgggaaca tcaagatgcc cggtgtccat gccgttgatc accgcctgga aaggatcgag
     2521 gagagtgaca atgaaaccta cgtagtgcaa agagaagtgg cagttgccaa atacagcaac
     2581 cttggtggtg gcatggacga gctgtacaag ggaagcggag ctactaactt cagcctgctg
     2641 aagcaggctg gagacgtgga ggagaaccct ggacctatga agggccgaaa gcctcgggac
     2701 ctggaacttc cacttagccc aagcctgctg ggtggccagg gacctgaacg gactccagga
     2761 tcaggaacaa gctctggtct tcaggcaccg gggccagcgc tttcgcccag caagcgtagc
     2821 ggtctcgagg acccagcgac gccatcgaag aagccacgta ccccatcggt gtcgtcaagg
     2881 ctggagcgcc tgaccctcca gtccagcttt caattcccat ccactagtat ggtgagcaag
     2941 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3001 ggccacaagt tcagcgtccg cggcgagggc gagggcgatg ccaccaacgg caagctgacc
     3061 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3121 ttcggctacg gcgtggcctg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3181 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatctcttt caaggacgac
     3241 ggtacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3301 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3361 aacttcaaca gccactacgt ctatatcacg gccgacaagc agaagaactg catcaaggct
     3421 aacttcaaga tccgccacaa cgttgaggac ggcagcgtgc agctcgccga ccactaccag
     3481 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccat
     3541 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3601 gtgaccgccg cctagtcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3661 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3721 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3781 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3841 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3901 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3961 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4021 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4081 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4141 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4201 cctccccgca tcgataccgt cgacctcgac ctcgactgtg ccttctagtt gccagccatc
     4261 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4321 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4381 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacagctagc gtttaaactt
     4441 aagctttcta gaagatgggc gggagtcttc tgggcaggct taaaggctaa cctggtgtgt
     4501 gggcgttgtc ctgcagggga attgaacagg tgtaaaattg gagggacaag acttcccaca
     4561 gattttcggt tttgtcggga agttttttaa taggggcaaa taaggaaaat gggaggatag
     4621 gtagtcatct ggggttttat gcagcaaaac tacaggttat tattgcttgt gatccgcctc
     4681 ggagtatttt ccatcgaggt agattaaaga catgctcacc cgagttttat actctcctgc
     4741 ttgagatcct tactacagta tgaaattaca gtgtcgcgag ttagactatg taagcagaat
     4801 tttaatcatt tttaaagagc ccagtacttc atatccattt ctcccgctcc ttctgcagcc
     4861 ttatcaaaag gtattttaga acactcattt tagccccatt ttcatttatt atactggctt
     4921 atccaacccc tagacagagc attggcattt tccctttcct gatcttagaa gtctgatgac
     4981 tcatgaaacc agacagatta gttacataca ccacaaatcg aggctgtagc tggggcctca
     5041 acactgcagt tcttttataa ctccttagta cactttttgt tgatcctttg ccttgatcct
     5101 taattttcag tgtctatcac ctctcccgtc aggtggtgtt ccacatttgg gcctattctc
     5161 agtccaggga gttttacaac aatagatgta ttgagaatcc aacctaaagc ttaactttcc
     5221 actcccatga atgcctctct cctttttctc catttataaa ctgagctatt aaccattaat
     5281 ggtttccagg tggatgtctc ctcccccaat attacctgat gtatcttaca tattgccagg
     5341 ctgatatttt aagacattaa aaggtatatt tcattattga gccacatggt attgattact
     5401 gcttactaaa attttgtcat tgtacacatc tgtaaaaggt ggttcctttt ggaatgcaaa
     5461 gttcaggtgt ttgttgtctt tcctgaccta aggtcttgtg agcttgtatt ttttctattt
     5521 aagcagtgct ttctcttgga ctggcttgac tcatggcatt ctacacgtta ttgctggtct
     5581 aaatgtgatt ttgccaagct tcttcaggac ctataatttt gcttgacttg tagccaaaca
     5641 caagtaaaat gattaagcaa caaatgtatt tgtgaagctt ggtttttagg ttgttgtgtt
     5701 gtgtgtgctt gtgctctata ataatactat ccaggggctg gagaggtggc tcggagttca
     5761 agagcacaga ctgctcttcc agaagtcctg agttcaattc ccagcaacca catggtggct
     5821 cacaaccatc tgtaatggga tctgatgccc tcttctggtg tgtctgaaga ccacaagtgt
     5881 attcacatta aataaataaa tcctccttct tcttcttttt ttttttttta aagagaatac
     5941 tgtctccagt agaatttact gaagtaatga aatactttgt gtttgttcca atatggtagc
     6001 caataatcaa attactcttt aagcactgga aatgttacca aggaactaat ttttatttga
     6061 agtgtaactg tggacagagg agccataact gcagacttgt gggatacaga agaccaatgc
     6121 agactttaat gtcttttctc ttacactaag caataaagaa ataaaaattg aacttctagt
     6181 atcctatttg tttaaactgc tagctttact taacttttgt gcttcatcta tacaaagctg
     6241 aaagctaagt ctgcagccat tactaaacat gaaagcaagt aatgataatt ttggatttca
     6301 aaaatgtagg gccagagttt agccagccag tggtggtgct tgcctttatg cctttaatcc
     6361 cagcactctg gaggcagaga caggcagatc tctgagtttg agcccagcct ggtctacaca
     6421 tcaagttcta tctaggatag ccaggaatac acacagaaac cctgttgggg aggggggctc
     6481 tgagatttca taaaattata attgaagcat tccctaatga gccactatgg atgtggctaa
     6541 atccgtctac ctttctgatg agatttgggt attatttttt ctgtctctgc tgttggttgg
     6601 gtcttttgac actgtgggct ttctttaaag cctccttcct gccatgtggt ctcttgtttg
     6661 ctactaactt cccatggctt aaatggcatg gctttttgcc ttctaagggc agctgctgag
     6721 atttgcagcc tgatttccag ggtggggttg ggaaatcttt caaacactaa aattgtcctt
     6781 taattttttt tttaaaaaat gggttatata ataaacctca taaaatagtt atgaggagtg
     6841 aggtggacta atattaaatg agtccctccc ctataaaaga gctattaagg ctttttgtct
     6901 tatacttaac ttttttttta aatgtggtat ctttagaacc aagggtctta gagttttagt
     6961 atacagaaac tgttgcatcg cttaatcaga ttttctagtt tcaaatccag agaatccaaa
     7021 ttcttcacag ccaaagtcaa attaagaatt tctgactttt aatgttaatt tgcttactgt
     7081 gaatataaaa atgatagctt ttcctgaggc agggtctcac tatgtatctc tgcctgatct
     7141 gcaacaagat atgtagacta aagttctgcc tgcttttgtc tcctgaatac taaggttaaa
     7201 atgtagtaat acttttggaa cttgcaggtc agattctttt ataggggaca cactaaggga
     7261 gcttgggtga tagttggtaa aatgtgtttc aagtgatgaa aacttgaatt attatcaccg
     7321 caacctactt tttaaaaaaa aaagccaggc ctgttagagc atgcttaagg gatccctagg
     7381 acttgctgag cacacaagag tagttacttg gcaggctcct ggtgagagca tatttcaaaa
     7441 aacaaggcag acaaccaaga aactacagtt aaggttacct gtctttaaac catctgcata
     7501 tacacaggga tattaaaata ttccaaataa tatttcattc aagttttccc ccatcaaatt
     7561 gggacatgga tttctccggt gaataggcag agttggaaac taaacaaatg ttggttttgt
     7621 gatttgtgaa attgttttca agtgatagtt aaagcccatg agatacagaa caaagctgct
     7681 atttcgaggt ctcttggttt atactcagaa gcacttcttt gggtttccct gcactatcct
     7741 gatcatgtgc taggcctacc ttaggctgat tgttgttcaa ataaacttaa gtttcctgtc
     7801 aggtgatgtc atatgatttc atatatcaag gcaaaacatg ttatatatgt taaacatttg
     7861 tacttaatgt gaaagttagg tctttgtggg tttgattttt aattttcaaa acctgagcta
     7921 aataagtcat ttttacatgt cttacatttg gtggaattgt ataattgtgg tttgcaggca
     7981 agactctctg acctagtaac cctacctata gagcactttg ctgggtcaca agtctaggag
     8041 tcaagcattt caccttgaag ttgagacgtt ttgttagtgt atactagttt atatgttgga
     8101 ggacatgttt atccagaaga tattcaggac tatttttgac tgggctaagg aattgattct
     8161 gattagcact gttagtgagc attgagtggc ctttaggctt gaattggagt cacttgtata
     8221 tctcaaataa tgctggcctt ttttaaaaag cccttgttct ttatcaccct gttttctaca
     8281 taatttttgt tcaaagaaat acttgtttgg atctcctttt gacaacaata gcatgttttc
     8341 aagccatatt ttttttcctt tttttttttt tttttnggtt tttcgagaca gggtttctct
     8401 gtatagccct ggctgtcctg gaactcactt tgtagaccag gctggcctcg aactcagaaa
     8461 tccgcctgcc tctgcctcct gagtgccggg attaaaggcg tgcaccacca cgcctggcta
     8521 agttggatat tttgttatat aactataacc aatactaact ccactgggtg gatttttaat
     8581 tcagtcagta gtcttaagtg gtctttattg gcccttcatt aaaatctact gttcactcta
     8641 acagaggctg ttggtactag tggcacttaa gcaacttcct acggatatac tagcagatta
     8701 agggtcaggg atagaaacta gtctagcgtt ttgtatacct accagcttta tactaccttg
     8761 ttctgataga aatatttcag gacatctagc ttatcgatac cgtcgacggt atcgataagc
     8821 ttgatatcga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     8881 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     8941 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     9001 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     9061 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     9121 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     9181 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     9241 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     9301 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcaggtcc tcgccatgga
     9361 tcctgatgat gttgttgatt cttctaaatc ttttgtgatg gaaaactttt cttcgtacca
     9421 cgggactaaa cctggttatg tagattccat tcaaaaaggt atacaaaagc caaaatctgg
     9481 tacacaagga aattatgacg atgattggaa agggttttat agtaccgaca ataaatacga
     9541 cgctgcggga tactctgtag ataatgaaaa cccgctctct ggaaaagctg gaggcgtggt
     9601 caaagtgacg tatccaggac tgacgaaggt tctcgcacta aaagtggata atgccgaaac
     9661 tattaagaaa gagttaggtt taagtctcac tgaaccgttg atggagcaag tcggaacgga
     9721 agagtttatc aaaaggttcg gtgatggtgc ttcgcgtgta gtgctcagcc ttcccttcgc
     9781 tgaggggagt tctagcgttg aatatattaa taactgggaa caggcgaaag cgttaagcgt
     9841 agaacttgag attaattttg aaacccgtgg aaaacgtggc caagatgcga tgtatgagta
     9901 tatggctcaa gcctgtgcag gaaatcgtgt caggcgatct ctttgtgaag gaaccttact
     9961 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
    10021 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
    10081 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcagatcct agagctcgct
    10141 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
    10201 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
    10261 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
    10321 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
    10381 ctgaggcgga aagaaccagc tggggctcga gggggggccc ggtacccagc ttttgttccc
    10441 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    10501 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    10561 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10621 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10681 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10741 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10801 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10861 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10921 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10981 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11041 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11101 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11161 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11221 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11281 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    11341 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11401 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11461 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11521 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11581 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    11641 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11701 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11761 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11821 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11881 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11941 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    12001 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    12061 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    12121 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    12181 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    12241 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    12301 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    12361 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    12421 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    12481 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    12541 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    12601 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
    12661 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    12721 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    12781 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    12841 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    12901 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    12961 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
    13021 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
    13081 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    13141 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    13201 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
    13261 ctcactatag ggcgaattgg agctccaccg cggcaggccc tccgagcgtg gtggagccgt
    13321 tctgtgagac agccgggtac gagtcgtgac gctggaaggg gcaagcgggt ggtgggcagg
    13381 aatgcggtcc gccctgcagc aaccggaggg ggagggagaa gggagcggaa aagtctccac
    13441 cggacgcggc catggctcgg gggggggggg srgcggagga gcgcttccgg ccgacgtctc
    13501 gtcgctgatt ggcttctttt cctcccgccg tgtgtgaaaa cacaaatggc gtgttttggt
    13561 tggcgtaagg cgcctgtcag ttaacggcag ccggagtgcg cagccgccgg cagcctcgct
    13621 ctgcccactg ggtggggcgg gaggtaggtg gggtgaggcg agctggacgt gcgggcgcgg
    13681 tcggcctctg gcggggcggg ggaggggagg gagggtcagc gaaagtagct cgcgcgcgag
    13741 cggccgccca ccctcccctt cctctggggg agtcgtttta cccgccgccg gccgggcctc
    13801 gtcgtctgat tggctctcgg ggcccagaaa actggccctt gccattggct cgtgttcgtg
    13861 caagttgagt ccatccgccg gccagcgggg gcggcgagga ggcgctccca ggttccggcc
    13921 ctcccctcgg ccccgcgccg cagagtctgg ccgcgcgccc ctgcgcaacg tggcaggaag
    13981 cgcgcgctgg gggcggggac gggcagtagg gctgagcggc tgcggggcgg gtgcaagcac
    14041 gtttccgact tgagttgcct caagaggggc gtgctgagcc agacctccat cgcgcactcc
    14101 ggggagtgga gggaaggagc gagggctcag ttgggctgtt ttggaggcag gaagcacttg
    14161 ctctcccaaa gtcgctctga gttgttatca gtaagggagc tgcagtggag taggcgggga
    14221 gaaggccgca cccttctccg gaggggggag gggagtgttg caataccttt ctgggagttc
    14281 tctgctgcct cctggcttct gaggaccgcc ctgggcctgg gagaatccct tccccctctt
    14341 ccctcgtgat ctgcaactcc agtctttcta ggtagggcgc agtagtccag ggtttccttg
    14401 atgatgtcat acttatcctg tccctttttt ttccacagct cgcggttgag gacaaactct
    14461 tcgcggtctt tccagtttcg aacacgtggg cgcgcccccg ggctagtgga tccggaaccc
    14521 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg acctgcagga
    14581 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
    14641 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
    14701 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
    14761 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
    14821 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
    14881 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
    14941 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
    15001 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
    15061 ctcctcttcc tcatctccgg gcctttcgac ctgcagccaa tatgggatcg gccattgaac
    15121 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
    15181 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
    15241 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
    15301 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
    15361 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
    15421 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
    15481 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
    15541 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
    15601 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc
    15661 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
    15721 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttg
//
