LOCUS       sequence-41618-e         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Ding Y, Ai HW, Hoi H, Campbell RE
  TITLE     Forster resonance energy transfer-based biosensors for 
            multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 
            activity in single cells.
  JOURNAL   Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 
            2011 Nov 28.
  PUBMED    22080726
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac202595g";
            journalName: "Anal Chem"; date: "2011-12-15- 15"; volume: "83"; 
            issue: "24"; pages: "9687-93"
COMMENT     Sequence Label: CaYin1
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             64..86
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             106..123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             127..159
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             172..192
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(332..350)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     600..619
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctgacgcttt ttatcgcact ctctactgtt tctccatacc cgtttttttg ggctagaaat
       61 aattttgttt aactttaaga aggagatata catatgcggg gttctcatca tcatcatcat
      121 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgagaacctg
      181 tacttccagg gctcgagcat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
      241 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
      301 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
      361 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
      421 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
      481 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
      541 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
      601 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
      661 gacggcgtgc tgaa
//
