LOCUS       sequence-416189-       13786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible plasmid for TurboID-based proximity labelling 
            experiment in planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Tan H, Zhou Y, Dinius E, Lozano-Duran R
  TITLE     The Ti-TAN plasmid toolbox for TurboID-based proximity labeling 
            assays in Nicotiana benthamiana.
  JOURNAL   J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610.
  PUBMED    38294100
REFERENCE   2  (bases 1 to 13786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610."
COMMENT     Sequence Label: pGW-GFP-GOI-TurboID
FEATURES             Location/Qualifiers
     source          1..13786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1178..1180,1181..1183,1184..1891)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(1484..1503)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1831..1852
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1895..2019
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2056..2061,2062..2079,2080..2086)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2140..2799
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2206..2225)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3141..3446
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3325..3344
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3487..3611)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3631..3657
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3661..3687
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3691..3717
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3724..4680
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     terminator      4708..4960
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4993..5009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4993..5009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5006..5028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5017..5033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5041..5047,5048..5065,5066..5071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5086..5107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5143..5395)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5546..6571)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6623..6802)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7308..7332
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7853..8644
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8890..9478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9379..9398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9632..9649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9664..9804
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9730..9749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9890..9912
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10148..10342
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10408..11481)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11910..12539)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aggatccatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
     1381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact cacggcatgg acgagctgta catcacaagt ttgtacaaaa aagctgaacg
     1921 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     1981 tacataatac tgtaaaacac aacatatcca gtcactatgg cggcccctcg aggggccgca
     2041 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     2101 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     2161 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     2221 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     2281 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     2341 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     2401 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     2461 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     2521 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     2581 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     2641 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     2701 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     2761 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta
     2821 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     2881 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     2941 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     3001 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     3061 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     3121 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     3181 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     3241 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     3301 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     3361 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     3421 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     3481 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     3541 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     3601 tacaaagtgg ttcgatctag aggatccatg tacccgtatg atgttccgga ttacgctggc
     3661 tatccctacg acgtgcccga ctatgccggg tacccctatg acgtcccaga ctacgcagct
     3721 agcaaagaca atactgtgcc tctgaagctg atcgctctcc tggctaatgg cgagttccat
     3781 agtggcgaac agctgggaga aaccctgggc atgtccaggg ccgctatcaa caagcacatt
     3841 cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg ctactctctg
     3901 cccgagccta tcccgctgct gaacgctaaa cagattctgg gacagctgga cggcgggagc
     3961 gtggcagtcc tgcctgtggt agactccacc aatcagtacc tgctggatcg aatcggcgag
     4021 ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg aagcagaggg
     4081 aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg gcgcctgaag
     4141 cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg
     4201 cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta tctgcaggat
     4261 agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc tgcacagatc
     4321 gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag
     4381 ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct ggccgctacg
     4441 ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct ggctccatat
     4501 ctgccacggt gggagaagct ggataacttc atcaatagac ccgtgaagct gatcattggg
     4561 gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct gctgctggaa
     4621 caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag
     4681 tagagcggcc cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     4741 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     4801 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     4861 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     4921 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt tccggaacca
     4981 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5041 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5101 cacattaggc tgaattaggc gcgcctattt ctattaattc ccgatctagt aacatagatg
     5161 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt
     5221 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt
     5281 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa
     5341 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga
     5401 aattcggggg atcgatcccg cgaactgtgg acgagaactg tgccaccaag cgtaaggccg
     5461 ttctctcgca tttgccttgc taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc
     5521 agctcttgtt cggtcggcat ctactctatt cctttgccct cggacgagtg ctggggcgtc
     5581 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     5641 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     5701 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     5761 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc
     5821 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     5881 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     5941 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     6001 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     6061 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     6121 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     6181 tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa
     6241 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     6301 tgcaataggt caggctctcg ctgaatgccc caatgtcaag cacttccgga atcgggagcg
     6361 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     6421 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     6481 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     6541 cagacgtcgc ggtgagttca ggctttttca tatcttattg ccccccggga tcagatctgg
     6601 attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg
     6661 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg
     6721 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt
     6781 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
     6841 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcttgat cccctgcgcc
     6901 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     6961 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
     7021 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
     7081 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
     7141 ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg
     7201 aagctgatcc gtcgagatta atagaaataa attcagccta attcggcgtt aattcagtac
     7261 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     7321 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     7381 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     7441 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     7501 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     7561 cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     7621 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     7681 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     7741 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     7801 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga
     7861 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     7921 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     7981 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     8041 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     8101 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     8161 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     8221 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     8281 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     8341 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     8401 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     8461 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     8521 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     8581 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     8641 ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     8701 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
     8761 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac
     8821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9001 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     9061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     9301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     9361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     9601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9661 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     9721 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     9781 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     9841 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     9901 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
     9961 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
    10021 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
    10081 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
    10141 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
    10201 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
    10261 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
    10321 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
    10381 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
    10441 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
    10501 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
    10561 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
    10621 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
    10681 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
    10741 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
    10801 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
    10861 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
    10921 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
    10981 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
    11041 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
    11101 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
    11161 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
    11221 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
    11281 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
    11341 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
    11401 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
    11461 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
    11521 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
    11581 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
    11641 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
    11701 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
    11761 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
    11821 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
    11881 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
    11941 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
    12001 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
    12061 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
    12121 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
    12181 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
    12241 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
    12301 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
    12361 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
    12421 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
    12481 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
    12541 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
    12601 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
    12661 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
    12721 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
    12781 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
    12841 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
    12901 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    12961 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    13021 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    13081 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    13141 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    13201 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    13261 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    13321 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    13381 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    13441 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    13501 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    13561 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    13621 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
    13681 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga
    13741 accctgtggt tggcatgcac atacaaatgg acgaacggat aaacct
//
