LOCUS       sequence-416219-       10188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with mTagBFP2 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LAAPTW
FEATURES             Location/Qualifiers
     source          1..10188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        5040..5539
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5075..5098)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5472..5491
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5557..6267
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(6331..6351)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6749..6760)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6950..7583
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7712..7728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7712..7728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7725..7747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7736..7752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7760..7766,7767..7784,7785..7790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7805..7826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7943..7960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8114..8702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8194..8213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8873..9664,9665..9733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9496..9515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9734..9838)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9886..10020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9923..9942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9977..9996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10188,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgttccat gtccttatat ggactcatct ttgcctattg
     2221 cgacacacac tcaatgaaca cctactacgc gctgcaaaga gccccgcagg cctgaggtgc
     2281 ccccacctca ccactcttcc tatttttgtg taaaaatcca gcttcttgtc accacctcca
     2341 aggaggggga ggaggaggaa ggcaggttcc tctaggctga gccgaatgcc cctctgtggt
     2401 cccacgccac tgatcgctgc atgcccacca cctgggtaca cacagtctgt gattcccgga
     2461 gcagaacgga ccctgcccac ccggtcttgt gtgctactca gtggacagac ccaaggcaag
     2521 aaagggtgac aaggacaggg tcttcccagg ctggctttga gttcctagca ccgccccgcc
     2581 cccaatcctc tgtggcacat ggagtcttgg tccccagagt cccccagcgg cctccagatg
     2641 gtctgggagg gcagttcagc tgtggctgcg catagcagac atacaacgga cggtgggcct
     2701 agacccaggc tgtgtagacc cagccccccc gccccgcagt gactaggtca cccactaacg
     2761 ccccaggcct ggtcttggct gggcgtgact gttaccctca aaagcaggca gctccagggt
     2821 aaaaggtgcc ctgccctgta gagcccacct tccttcccag ggctgcggct gggtaggttt
     2881 gtagccttca tcacgggcca cctccagcca ctggaccgct ggcccctgcc ctgtcctggg
     2941 gagtgtggtc ctgcgacttc taagtggccg caagccacct gactccccca acaccacact
     3001 ctacctctca agcccaggtc tctccctagt gacccaccca gcacatttag ctagctgagc
     3061 cccacagcca gaggtcctca ggccctgctt tcagggcagt tgctctgaag tcggcaaggg
     3121 ggagtgactg cctggccact ccatgccctc caagagctcc ttctgcagga gcgtacagaa
     3181 cccagggcct tggcacccgt gcagaccctg gcccacccca cctgggcgct cagtgcccaa
     3241 gagatgtcca caactaggat gtcccgcggt gggtgggggg ccagagagac gggcaggccg
     3301 ggggcaggcc tggccatgcg gggccgaacc gggcactgcc cagcgtgggg cgcgggggcc
     3361 acggcgcgcg cccccagccc cccggcccag caccccaagg cggccaacgc caaaactctc
     3421 cctcctcctc ttcctcaatc tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga
     3481 gggggtaaaa aaatgctgca ctgtgcggcg aagccggtga gtgagcggcg cggggccaat
     3541 cagcgtgcgc cgttccgaaa gttgcctttt atggctcgaa cggccgcggc ggcgccctat
     3601 aaaacccagc ggcgcgacgc gccaccaccg ccgagaccgc gtccgccccg cgagcacaga
     3661 gcctcgcctt tgccgatccg ccgcccgtcc aggctcgcat ctctccttca cgcgcccgcc
     3721 gccttacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     3781 gtgcctcctg aactacgtcc gccgtctagg taagtttaga gctcaggtcg agaccgggcc
     3841 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     3901 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcag
     3961 cgcttcgact agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc
     4021 gttgcctgca catcttggaa acacttgctg ggattacttc gacttcttaa cccaacagaa
     4081 ggctcgagaa ggtatattgc tgttgacagg atccacatta acaactttaa gtccagcaat
     4141 ttgagttaag ggtgttgctc tcaatgattt cactaatggt tcaattttta atttcttttc
     4201 ttctggttca aaattcaagt ttcaagtgaa agtgtaatat gcacccattt ctttcaataa
     4261 atcttctaaa tagtccacta atgttttatt ttgtttttta caaaatcaag cagcctctgc
     4321 tatcaatata gaaccttgta ttccatcttt atctctagct gagtcatcaa ttacatatcc
     4381 ataactttct tcataagcaa aaacaaaatt taatccgtta tcttcttctt tagcaatttc
     4441 tctacccatt catttcaatc cagttaaagt ttttacaatc ttaactccat atttttcctg
     4501 agcgattcta tcacccaaat cacttgttac aaaacttgaa tatagagccg gattttttgg
     4561 aatgctattt aagcgtttta gatttgataa ttttcaatca attaaaattg gtcctgtttg
     4621 atttccatct aatcttacaa aatgaccatc atgttttatt gccattccaa atctgtcagc
     4681 atctgggtca ttcataataa taatatctgc atcatgttta ataccatatt caagcggtat
     4741 ttttcatgca ggatcaaatt ctggatttgg atttacaaca tttttcaatg tttcatcttc
     4801 aaatgcacga tcttcaccct caataacgtt atatcctgat tcacgtaatc tttttggggt
     4861 aaatttagtt cctgttccat taactgcgct aaaaataggg ccctgcctcg gacttcaagg
     4921 ggctagaatt cgagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat
     4981 acatttttac aaagctgaat taaaatggta taaattaaat cacttttttc aattctaccg
     5041 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5101 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5161 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5221 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5281 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5341 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5401 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5461 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5521 ctcatctccg ggcctttcga cctttaatta accaccatgg tgtctaaggg cgaagagctg
     5581 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     5641 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     5701 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     5761 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     5821 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     5881 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     5941 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggcgttcacc
     6001 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     6061 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
     6121 aagaacctca agatgcctgg cgtgtactat gtggactaca gactggaaag aatcaaggag
     6181 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     6241 cctagcaaac tggggcacaa gctaaattag gtttaaacat caacctctgg attacaaaat
     6301 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6361 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6421 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6481 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6541 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6601 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6661 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     6721 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6781 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6841 gatctccctt tgggccgcct ccccgcctgg cctgcagggg tacctttaag accaatgact
     6901 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     6961 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7021 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     7081 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     7141 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     7201 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     7261 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     7321 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     7381 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7441 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7501 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7561 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7621 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt
     7681 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7741 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7801 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7861 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7921 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7981 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8041 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8281 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8341 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8401 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8461 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8521 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8581 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8641 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8701 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8761 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8821 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8881 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8941 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9001 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9061 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9121 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9181 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9241 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9301 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9361 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9421 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9481 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9541 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9601 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9661 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9721 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9781 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9841 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
     9901 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9961 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    10021 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    10081 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    10141 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtt
//
