LOCUS       sequence-416229-       10383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with 
            His14-tagged Cse2 in pET45b(+).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Altae-Tran H, Kannan S, Suberski AJ, Mears KS, Demircioglu FE, 
            Moeller L, Kocalar S, Oshiro R, Makarova KS, Macrae RK, Koonin EV, 
            Zhang F
  TITLE     Uncovering the functional diversity of rare CRISPR-Cas systems with 
            deep terascale clustering.
  JOURNAL   Science. 2023 Nov 24;382(6673):eadi1910. doi: 
            10.1126/science.adi1910. Epub 2023 Nov 23.
  PUBMED    37995242
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-24"; volume: "382(6673):eadi1910. doi"; pages: " 
            10.1126/science.adi1910. Epub 2023 Nov 23"
COMMENT     Sequence Label: pHS1477
FEATURES             Location/Qualifiers
     source          1..10383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(582..1373,1374..1442))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1205..1224
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1443..1535)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1616..2204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2105..2124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2358..2375
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2390..2529
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2455..2474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2615..2637
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2631..2822)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3394..4476)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4438..4457
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4477..4554)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4760..4779
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4867..4886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4867..4885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4886..4910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4925..4947
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6650..6670
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      10311..10358
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat
      481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
      661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
      721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
      781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
      841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
      901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
      961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg
     1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac
     1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2401 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2461 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2521 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2581 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2641 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2701 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2761 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     2821 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     2881 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2941 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     3001 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     3061 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccat actttacgaa
     3121 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3181 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3241 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc tagtcatgcc ccgcgcccac
     3301 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     3361 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3421 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3481 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     3541 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     3601 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     3661 tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     3721 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     3781 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     3841 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     3901 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     3961 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     4021 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     4081 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     4141 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     4201 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     4261 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     4321 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     4381 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     4441 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     4501 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
     4561 ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     4621 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     4681 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     4741 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     4801 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg
     4861 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
     4921 taattttgtt taactttaag aaggagatat accatgtatt gcgcggcggt ttgctttccg
     4981 cagacaaaat atcagcaaag gggaactgct ctaaagaaac cgctcgtcgg tctgcgtaag
     5041 atgggtgtgg aagcggctgc atggaacacc ctgaaggtta ctagagatcg ccctaagttg
     5101 acctttccgg atctgatcac tccgcaaagc aaattcctgg ataacgactt atggctgaag
     5161 tacaaagtgc caattgagca gaaagaacac gtcatgtata acctgttgtg cgataactgg
     5221 gttaatgttg tttacttgag cggtaaaccg gaccgtattt ccttagtcca aaccctgaag
     5281 gacgcccatt gtctgcaatt ggcgtattct aacccgatgg atcgtttcac cgtgttccgc
     5341 ttcctgttgg cgttgggtta ctggtgtttc gcgaacacga acgtcgagcc ggagcccgac
     5401 aaaccactgc cggtgagctg gattccgtgg ctggaggaaa ataaagagta cttcgagctg
     5461 ttcggtgatg gtaaacgttt cttccaagct gacccgagca gccgtattcg tgcgattact
     5521 gacctgatcc atgaaattcc gaccgctcac aacctgtgcc attttaagca cgtgaccgac
     5581 tatatcgacg gcctgtgtga agcctgctgc ataaaaggtc tgttgcgtct gccggtattt
     5641 accaccgttg gcggtcgtgg cattggcgca ggtatcaata acacgccacc gttttatctg
     5701 ctgtggcatg cgaacgactt agcgggcatg ttggcgcaga attggcagcc ttgggacaac
     5761 atgggcatcc cggcgtggct gggctctttt cagaaagagt cgcgggaagt tggcctgctt
     5821 gcgggtatga cctggcttcc gcgtaaagtt tacctgcacg atccggttcc gggtcaggct
     5881 gcgtgctgca gctgtggttt gccgagtgaa gctctggttt actcatgctc cattgaggtg
     5941 gaaccagtgc caaagggctt ggagtggaag gacccgcacg gcgtttacac cgaccagggc
     6001 aaatccctgc agtccaaaat caagctgatg agcaatgacc gctatacctt cgcggaccgt
     6061 gactggtatt ctccgttgtt ctcgtatctt cacgccgaag gtaatagccg ccagggtaag
     6121 ctgtggctgg tgggttttgc gagcgataaa gcaaaatcta tcgacatctg ggataagatc
     6181 atcgagctgg agggcaccga caccaatgac gaactgttgg cgcaactcgc caatcgtgca
     6241 accgcgctga atgcaatgcg taaaaagccg ctacgcggtg attttaaaaa gagcgttggc
     6301 accccgcaga ttgcggatat catcccgcat gcggaaaacc gcatcgccat taacgcaggc
     6361 aaaatgacgg aaaaccgtgg ttactcttgg caagatgccg atacggagta cggagaactg
     6421 ctgaccaagg tggcatactc gctggagccg gcgcaaacgg tggatgctcg tctgaagcgc
     6481 ggtaacttta tcagccgtaa gccgtggccg attatccccg agagcaaaac aaaacccgca
     6541 gagggggacc agaatgaata gaggaagcaa gcatcaccat cattcaggcc atcaccatac
     6601 cggacaccac catcattcag gcagtcatca ccataccggg ggctcaggcg agaacctgta
     6661 ttttcagggt tctggtggaa atagaggaac cgttgacttc attgcaagct tggagaattt
     6721 aaaagaagga gatctaggga tactcagaaa gttacgtggt gcgcgtctgg acgaaaaact
     6781 tccgggtttt gatctgttca gcgcgctgtg gtggccattg cgtcaaaaaa accagcgcgc
     6841 accgaagcgc gaggtggctt ggctgattgc caaattgttt gcagaattcc gctttgagca
     6901 acgtgagggc gcgacgctgc cgattctgat gggcggtatc tgccgtaagt tggaaccgaa
     6961 gaaagaactg ccgcgtgtgt tggctcgttt cgaccagctg gcgagcctgg acatcatgca
     7021 gatggaagag ccgctgtccg tgattatggg catcctgcgc aagcaccagc aggtctgtct
     7081 ggactgggtt ggtctgaccg atgttttgtc gttctgggaa caagagccgg ttaaacgtga
     7141 gtggtctgat agctttatca aagcgtacaa gatcaacaag gaggattcag atgttgattg
     7201 aaattcatat gattcagaat cactcacccg ctaatctaaa cagggacgat ttaggcgctc
     7261 caaaaacgtg ctatttcggt ggcgtgctgc gtagccgcat ctcctctcag tgtattaagc
     7321 gcagcatccg tacgagcaat gacttcaaag cattgctcgg cggtgtacgt accagacgtc
     7381 tggcggatct gattcagcaa gaggcgggcg agacagagtg ctggaaaaag gctcaagaga
     7441 tcctgaataa gtgcggcttc aaaaacaagg acgacaatac caaaatgttg gtctttatga
     7501 gcaaagacaa gatcaaagac cttgcgcgta ttgttttgga caactcgctg ggcctgaccg
     7561 aagccgccca acaggtggcg aacgtgatcg ctcaggctac cctagctccg gacatcgcgc
     7621 tttgcggtcg tatgctggaa ccgaatgata aggacaagga caagaaggtg aagtggtcaa
     7681 acactaccgt tgaggcagcg ttgcaagtgg cgcatgcaat tagcacccat attgcccgtc
     7741 cggaaatcga ctacttcgtg gccgcggatg atgttccggg tgaagatgcg ggtgcgggcc
     7801 acatcggcga atctatgttt gcaagcgcat gtttttataa atacttcagc atcgattggg
     7861 aacaactggt taagaacttg aaaggtgata ccaacctggc ggctcacacc gtcggagcct
     7921 ttctgctggc ggcggcgaaa accaatccga gcggtaaaca gaatagcttt gccgcgcaca
     7981 actacccgga tggtattctg gtcgagttca agaactcccc gatttcctat gcaaatgctt
     8041 ttgttcgccc agttagcgtt gttaaagagt cggatttggt cgagcagtcc atcggtcagc
     8101 tgagcaacta cgtgaacgac atccgcttgg gctactatga tgaacagagc ccggtgattg
     8161 gtttctggtt ttccccgaac aaccgttatc cgctgggtta caaacacagc aaactggcga
     8221 gccgtaatat tggtaacctg aacgaactcg tgggtgcagt tctggattac atcggcggct
     8281 tcaagtggga agaagttcag aaaagcaagg cttatattgg cggctaacat gagtgcgcca
     8341 cccaacacct tattcttgag gttagaaggg gctctacaat catggggatc gaacgaagcg
     8401 aagttcgccc tgcgccggac cgcagacgcg ccaactaaat ccggcgttct gggcctgctg
     8461 tgcgcggcta tgggcatcgg tcgtgccgag gcggcggact cttggctgcc gaaactggct
     8521 aatctgcgta tgggtgttcg tatcgatcgt ccgggcatcc gctggtggga ttttcacacc
     8581 gtaggcgcag gccaacgtat gcgtatggcg gagctgaagg cgccaaagaa gccgagcatg
     8641 gtgggcgcag cgctggcgga aaccctgacc ccgagcaaag tcaaaacccg tgcggaaacc
     8701 ttgttgtccc gccgtgagta cctcgcggac gctagcttcc tggtggcgtt gcaaggtgag
     8761 ccggaacttg tcgcaaagct gtcggcggcc ttagccaaac cggtttgggc gatttatttg
     8821 ggtcgtaaga gctgtccgcc atctcgcccc gtgtgcgaac atccgccggg cttttataac
     8881 accctggaag aagcgctgag cgcagttccg ctacaaaaac gttggcataa cgagccgctg
     8941 ccgcagattc tgccgtgtgt tatggactgg attccgggtt atgatggtga gcacgctccg
     9001 gatgatgcgg agatccacta cgacttgccg gtgagcttcc agccgcctcg tcatctgccg
     9061 cgttttgtta ttcgtcggga actggtcgtg ggtgaggacg ttcaggtttc cagagaaact
     9121 ggtacgagcg tctggcgtcc taagggtacg cgtgcggact acaacaacag cgaatacaaa
     9181 aaggtgcgcg ccgagcgcct ggtgatggat cacgctgcct gcatggtttg caaagcaccg
     9241 gcaaccaccg tgcagcacgt gaattatcgt cgcgctggcg gtaaagagat cccggaggat
     9301 ttaagagctt tgtgccgcct gtgccatgac gcgtgtacca tgttagaata cggctctggt
     9361 atgaccacga atcgtatcga cccgtgcgat ccgatttgga gagaacgtat cctcgctaag
     9421 cgcaaggaga ttgttgaatt tcgtagccgc ggtcagcgtt tccgcaaaat gaagccagag
     9481 gaggaaaacg gatgatctac ttgtccaggc tattgatcga tactggaggt aatccagata
     9541 gacctaggcc cgggcgcaaa tggctcgaca acatttataa cgtgcatcgt cgtctgagca
     9601 tggcatttcc gagcggtctg cgccgtgaac aggacccgca ttttctgaag ccgttttctc
     9661 cgaatgactt ccagaaaacg ccattcttgt ttcgtgtcga caataatatc gacggcaacg
     9721 ataaacgtgc gattatcatc gtgcaaagcg tcctggagcc cgactgggat tactgctttc
     9781 aaaacgcctt agacttcttg gcggcgccac cggaaaccaa agagtacaac ccggaattta
     9841 aagcgggtca gttgctccgg ttccgcctgc gtgttaatgc atccgtgcgt agacacatcc
     9901 cggagatggt tcagcaggat ggccagacca ttgaaactgg taagatcctg cacaagcgcg
     9961 tgagcctgac ctgggacgct agctcgaccc cggatcaggc tttagctgat tggctggcgg
    10021 cgaagagccc gaagttgggc ttcacgctgc agcgttgtga actgctgcaa ctgggttggg
    10081 tttatggtag caaaccggaa ccgaagaacg tgaaagttaa agaacaaggc caaggctatt
    10141 ggcgtgagca caaatacaac ccgctgcgct tccgtgccgc attgcttgag ggcgttctgg
    10201 aggttgatga tccgaagctg ttcctgaaaa ccctgagcag cggtattggt aaggcgaaga
    10261 gttttggttt cggcctgctg tccgttctgc caatcaggaa tgatggataa ctagcataac
    10321 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
    10381 gat
//
