LOCUS       sequence-41623-e        6689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Johnson BE, Glauser DA, Dan-Glauser ES, Halling DB, Aldrich RW, 
            Goodman MB
  TITLE     Alternatively spliced domains interact to regulate BK potassium 
            channel gating.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Nov 2.
  PUBMED    22049343
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Nov 2."
COMMENT     Sequence Label: mg175
FEATURES             Location/Qualifiers
     source          1..6689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3513..3529)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3513..3529)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     3'UTR           3552..3677
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(3757..3776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3758..3776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3786..3803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3786..3802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3795..3817)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3943..4398)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4080..4101)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4292..4311
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4425..4529
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4530..4598,4599..5390)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4748..4767)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5561..6149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6050..6069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6303..6320
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6437..6458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6473..6478,6479..6496,6497..6503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6511..6527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6516..6538
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6535..6551
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6535..6551
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6570..6590
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6572..6590
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           6631..6673
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
ORIGIN
        1 atgggcgaga tttactcgcc ttcgcagtcg aagggcttca atcaaccata tggatacccg
       61 atgaattgta accttagtcg agtattcatg gagatgactg aagaagatcg aaagtgcctg
      121 gaggagcgca aatattggtg ttttttactg agcagcatca ccacattctg cgcctcaatg
      181 atattggtcg taatatggcg agtggtgaca cacctgtgct gtcaacgccg agaaaaagag
      241 tttgtggagc ccatacctgc tccggaagcc gtccaaataa atatgaacgg gtcgaagcac
      301 gcgccaagtg aaaccgaccc gtttttgaag caacaagagg aaaaacactt gggatggatg
      361 acagaagcca aggactgggc tggcgagctg atatccggac aaagtctgac gggacggttt
      421 ctggtacttt tggtgttcat tttatcgatt ggatcgttga ttatttattt ttacgacgct
      481 agtttccaaa acttccaagt agaaacgtgc atcccgtggc aggacagtcc ctcgcaacaa
      541 atcgacctgg gcttcaacat attttttctc gtctattttt tcattagatt catcgccgcg
      601 tccgacaaag tttggtttct gcttgaaatg tacagctgga ttgatttttt cacgattccg
      661 ccaagcttcg tggcaattta cttgcagagg aattggctcg gattccgttt cctccgtgct
      721 cttcgcctca tgaccgtacc cgacattcta cagtacctca acatcctgaa aacatcttca
      781 tcaatccgat tgacacagtt ggtcacaatt ttcgtggcgg tttgtctgac tggagccggc
      841 ttggtgcact tactggaaaa ttctggggac ttcttcaaag gatttataaa tccgcacaga
      901 atcacttatg cggactctgt gtactttgta ctggtcacaa tgtctactgt cggatatgga
      961 gatatctatt gtacgacgct ttgcggccga cttttcatga ttttcttcat tctgttcggt
     1021 ttggccatgt ttgcaagtta cgtaccagaa attgccgatt tgattggaaa ccggcaaaaa
     1081 tacggtgggg agtacaaagg agagcacggg aagaagcaca tagtggtctg tggccatatc
     1141 acctacgatt cggtgtccca ttttcttcaa gatttcctac acgaggaccg tgatgacgtg
     1201 gatgtcgaag tggtgttttt gcatcgtgtc gtgccggatt tggagctgga aggcttgttt
     1261 aagcggcatt tcacaaaagt cgaatttttc acggggactg tcatggattc tctggatctt
     1321 agcagagtca agatcgggga cgccgacgcc tgcctggtcc ttgccaacaa gtacagtacc
     1381 aatccggacg ccgaggacgc cgcaaacatt atgcgtgtaa tttcgatcaa aaactactca
     1441 tcggacatca gagttattgt gcaactaatg caatatcaca ataaggccta ccttctaaat
     1501 atcccctcct gggactggaa acgaggtgat gacgtcatct gtctcgcgga gcttaagctc
     1561 ggattcattg ctcaaagctg tctggcaccc ggattctcta cgatgatggc taatttattt
     1621 gcaatgagaa gtttcaaaac gtcacaaacc acaccggact ggctgaacct gtacctttgc
     1681 ggtgccggaa tggaaatgta caccgacacg ctatcccact cgttcgtcgg aatgactttt
     1741 cctgaagctg tcgacctatt attcaaccgc ctggggcttc ttcttctggc gattgagctt
     1801 aaagatgagg aaaataaaga atgcaatatt gcaatcaatc caggcccaca tattgtcatc
     1861 cagcctcaaa ctcaaggatt tttcattgct cagagcgctg acgaggtgaa gcgggcgttt
     1921 ttctggtgta agcagtgtca tgacgatatt aaggacgttt ccttgatcaa aaaatgtaaa
     1981 tgcaaaaatt tggccctctt ccgtcgcaac accaaacact cgacagcggc ccgtgattat
     2041 tcggactttg acgctctgtt ttatcagaat gacgcaagag ccaccgatgt gctccagcag
     2101 ttccagccac aagcgccagc aggtccgatg ggccatttgg gccaacaagt tcagctaagg
     2161 atgattaatc aacagaggcc atcttccgga ggacgccgaa attcgatgag tattccgcca
     2221 gacggcaggg gtgtcgattt tagcaaggat tttgagcagc aattccaaga catgaaatat
     2281 gacagtactg gaatgttcca ctggtgtcca tcccgtaacc tagaggactg tgttctggag
     2341 cgccaccagg ccgctatgac cgtactcaac ggccacgtgg tggtttgttt gttcgctgat
     2401 caggattctc cgctaattgg gctccggaac ttcataatgc cactaagaag ctcaaacttc
     2461 cattatcatg agctcaaaca cgtggttatt gtgggagacc tggaatattt gaggaaagag
     2521 tggaagacgc tgtacaactt gccgaaaatc tcgattttga atggctcccc gctatctcga
     2581 gcagatttac gcgccgtcaa cattaacctc tgcgacatgt gtgtcataat ctctgcccgg
     2641 gtcccaaata ccgaagatac cactttggca gataaggaag cgattctggc atcattgaac
     2701 attaaagcta tgcaatttga tgataccctt ggctttttcc cgatgaggca tcaaactggc
     2761 gatcggagcc cgctgggctc cccgatttcc atgcagaaga agggcgccaa gtttgggacc
     2821 aatgtgccga tgattacgga gcttgttaac gactcaaatg tgcaattcct cgaccaggac
     2881 gacgacgacg atccggacac cgagctctac ctcacgcagc ccttcgcctg cggaactgcc
     2941 ttcgccattt cagtgttgga ttctttgatg agtactacct acttcaacga ctcggccctg
     3001 accctcatcc gaactctggt cactggtgga gccacaccgg agcttgaact tattctggca
     3061 gaaggcgccg ggctccgagg tggctacagt accccggaga ccctctcaaa cagagatcgg
     3121 tgccgaattg ctcagatttc gctgcaggat aacccgtacg atggagttgt gcacaacact
     3181 acctatggtg caatgttcac gatagctctg aggaggtacg gccaactttg tatcgggctg
     3241 tataggcttc acgaccagga taatccggac tctatgaagc gatatgttat cactaatcct
     3301 ccagcagaat tgaggattaa aaatactgat tacgtctacg tacttgaaca attcgatccg
     3361 ggactggagt acgagccggg caaacgacac ttttagggcc taattttatc cgattttctt
     3421 gctacaaact acgggttatc cgactccgaa ggaccaataa tatttgcaaa tgctcatttt
     3481 taggactttt tttgaattcc tgcagcccgg gggatccact agttctagac tcgaggatct
     3541 ggttaccact aaaccagcct caagaacacc cgaatggagt ctctaagcta cataatacca
     3601 acttacactt tacaaaatgt tgtcccccaa aatgtagcca ttcgtatctg ctcctaataa
     3661 aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa aaaaaaaacc cccccccccc
     3721 cccccggggn cgccaccgng ggggagctcc aattcgccct atagtgagtc gtattacgcg
     3781 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3841 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3901 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata
     3961 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     4021 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     4081 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     4141 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     4201 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     4261 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     4321 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     4381 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4501 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4561 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4621 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4681 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4741 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4801 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4861 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4921 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4981 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5041 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5101 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5161 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5221 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5281 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5341 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5401 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5461 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5701 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6181 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6241 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6301 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6361 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6421 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6481 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6541 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     6601 tgggtaccgg gccccccctc gaggtagctt cttgttcttt ttgcagaagc tcagaataaa
     6661 cgctcaactt tggcagaatc gatcccacc
//
