LOCUS       sequence-416271-       10088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PYL-HDAC5 H1006Y expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Yang B, Borgeaud AC, Buricova M, Aeschbach L, Rodriguez-Lima O, Ruiz
            Buendia GA, Cinesi C, Taylor AS, Baubec T, Dion V
  TITLE     Expanded CAG/CTG repeats resist gene silencing mediated by targeted 
            Epigenome editing.
  JOURNAL   Hum Mol Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255.
  PUBMED    34494094
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255."
COMMENT     Sequence Label: pAB-HDAC5(mut-H-Y)
FEATURES             Location/Qualifiers
     source          1..10088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      701..710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1244..1315
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             1319..1339
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1340..1364
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4736..4835)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4853..4871)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4876..4892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4876..4892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4994..5013)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5005..5814
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5072..5091)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5961..6549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6703..6720
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6879..6906)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6998..7084)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     7133..7155
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7147..7164
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7148..7164
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7180..7279
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7412..7714
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7596..7615
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7856..7880)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7921..7938)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7927..8038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(8858..9649,9650..9718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNIGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9481..9500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9719..9823)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9898..9917)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc gccaccatgg ccacccagga
      721 cgagttcacc cagctgagcc agtctatcgc cgagttccac acctaccagc tgggcaacgg
      781 ccggtgcagc agcctgctgg cccagagaat ccacgcccct cccgagacag tgtggtccgt
      841 cgtgcggaga ttcgaccggc cccagatcta caagcacttc atcaagagct gcaacgtgtc
      901 cgaggacttc gagatgagag tgggctgcac ccgggacgtg aacgtgatca gcggcctgcc
      961 cgccaacacc agcagagaga gactggacct gctggacgac gacagaagag tgaccggctt
     1021 cagcatcacc ggcggagagc accggctgcg gaactacaag agcgtgacca ccgtgcacag
     1081 attcgagaaa gaggaagaag aggaacggat ctggaccgtg gtgctggaaa gctacgtggt
     1141 ggacgtgccc gagggcaaca gcgaagagga cacccggctg ttcgccgaca ccgtgatccg
     1201 gctgaacctg cagaagctgg cctccatcac cgaggccatg aacgactaca aggacgacga
     1261 cgacaaggac tataaggacg atgatgataa ggattacaaa gatgacgacg ataagccccc
     1321 caagaagaag cggaaagtca caagtttgta caaaaaagca ggctcaatga actctcccaa
     1381 cgagtcggat gggatgtcag gtcgggaacc atccttggaa atcctgccgc ggacttctct
     1441 gcacagcatc cctgtgacag tggaggtgaa gccggtgctg ccaagagcca tgcccagttc
     1501 catggggggt gggggtggag gcagccccag ccctgtggag ctacgggggg ctctggtggg
     1561 ctctgtggac cccacactgc gggagcagca actgcagcag gagctcctgg cgctcaagca
     1621 gcagcagcag ctgcagaagc agctcctgtt cgctgagttc cagaaacagc atgaccacct
     1681 gacaaggcag catgaggtcc agctgcagaa gcacctcaag cagcagcagg agatgctggc
     1741 agccaagcag cagcaggaga tgctggcagc caagcggcag caggagctgg agcagcagcg
     1801 gcagcgggag cagcagcggc aggaagagct ggagaagcag cggctggagc agcagctgct
     1861 catcctgcgg aacaaggaga agagcaaaga gagtgccatt gccagcactg aggtaaagct
     1921 gaggctccag gaattcctct tgtcgaagtc aaaggagccc acaccaggcg gcctcaacca
     1981 ttccctccca cagcacccca aatgctgggg agcccaccat gcttctttgg accagagttc
     2041 ccctccccag agcggccccc ctgggacgcc tccctcctac aaactgcctt tgcctgggcc
     2101 ctacgacagt cgagacgact tccccctccg caaaacagcc tctgaaccca acttgaaagt
     2161 gcgttcaagg ctaaaacaga aggtggctga gcggagaagc agtcccctcc tgcgtcgcaa
     2221 ggatgggact gttattagca cctttaagaa gagagctgtt gagatcacag gtgccgggcc
     2281 tggggcgtcg tccgtgtgta acagcgcacc cggctccggc cccagctctc ccaacagctc
     2341 ccacagcacc atcgctgaga atggctttac tggctcagtc cccaacatcc ccactgagat
     2401 gctccctcag caccgagccc tccctctgga cagctccccc aaccagttca gcctctacac
     2461 gtctccttct ctgcccaaca tctccctagg gctgcaggcc acggtcactg tcaccaactc
     2521 acacctcact gcctccccga agctgtcgac acagcaggag gccgagaggc aggccctcca
     2581 gtccctgcgg cagggtggca cgctgaccgg caagttcatg agcacatcct ctattcctgg
     2641 ctgcctgctg ggcgtggcac tggagggcga cgggagcccc cacgggcatg cctccctgct
     2701 gcagcatgtg ctgttgctgg agcaggcccg gcagcagagc accctcattg ctgtgccact
     2761 ccacgggcag tccccactag tgacgggtga acgtgtggcc accagcatgc ggacggtagg
     2821 caagctcccg cggcatcggc ccctgagccg cactcagtcc tcaccgctgc cgcagagtcc
     2881 ccaggccctg cagcagctgg tcatgcaaca acagcaccag cagttcctgg agaagcagaa
     2941 gcagcagcag ctacagctgg gcaagatcct caccaagaca ggggagctgc ccaggcagcc
     3001 caccacccac cctgaggaga cagaggagga gctgacggag cagcaggagg tcttgctggg
     3061 ggagggagcc ctgaccatgc cccgggaggg ctccacagag agtgagagca cacaggaaga
     3121 cctggaggag gaggacgagg aagaggatgg ggaggaggag gaggattgca tccaggttaa
     3181 ggacgaggag ggcgagagtg gtgctgagga ggggcccgac ttggaggagc ctggtgctgg
     3241 atacaaaaaa ctgttctcag atgcccagcc gctgcagcct ttgcaggtgt accaggcgcc
     3301 cctcagcctg gccactgtgc cccaccaggc cctgggccgt acccagtcct cccctgctgc
     3361 ccctgggggc atgaagagcc ccccagacca gcccgtcaag cacctcttca ccacaggtgt
     3421 ggtctacgac acgttcatgc taaagcacca gtgcatgtgc gggaacacac acgtgcaccc
     3481 tgagcatgct ggccggatcc agagcatctg gtcccggctg caggagacag gcctgcttag
     3541 caagtgcgag cggatccgag gtcgcaaagc cacgctagat gagatccaga cagtgcactc
     3601 tgaataccac accctgctct atgggaccag tcccctcaac cggcagaagc tagacagcaa
     3661 gaagttgctc ggccccatca gccagaagat gtatgctgtg ctgccttgtg ggggcatcgg
     3721 ggtggacagt gacaccgtgt ggaatgagat gcactcctcc agtgctgtgc gcatggcagt
     3781 gggctgcctg ctggagctgg ccttcaaggt ggctgcagga gagctcaaga atggatttgc
     3841 catcatccgg cccccaggac accacgccga ggaatccaca gccatgggat tctgcttctt
     3901 caactctgta gccatcaccg caaaactcct acagcagaag ttgaacgtgg gcaaggtcct
     3961 catcgtggac tgggacattc accatggcaa tggcacccag caggcgttct acaatgaccc
     4021 ctctgtgctc tacatctctc tgcatcgcta tgacaacggg aacttctttc caggctctgg
     4081 ggctcctgaa gaggttggtg gaggaccagg cgtggggtac aatgtgaacg tggcatggac
     4141 aggaggtgtg gaccccccca ttggagacgt ggagtacctt acagccttca ggacagtggt
     4201 gatgcccatt gcccacgagt tctcacctga tgtggtccta gtctccgccg ggtttgatgc
     4261 tgttgaagga catctgtctc ctctgggtgg ctactctgtc accgccagat gttttggcca
     4321 cttgaccagg cagctgatga ccctggcagg gggccgggtg gtgctggccc tggagggagg
     4381 ctatgacttg accgccatct gtgatgcctc tgaggcttgt gtctcggctc tgctcagtgt
     4441 agagctgcag cccttggatg aggcagtctt gcagcaaaag cccaacatca acgcagtggc
     4501 cacgctagag aaagtcatcg agatccagag caaacactgg agctgtgtgc agaagttcgc
     4561 cgctggtctg ggccggtccc tgcgagaggc ccaagcaggt gagaccgagg aggccgagac
     4621 tgtgagcgcc atggccttgc tgtcggtggg ggccgagcag gcccaggctg cggcagcccg
     4681 ggaacacagc cccaggccgg cagaggagcc catggagcag gagcctgccc tgtgaaccca
     4741 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     4801 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     4861 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     4921 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     4981 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     5041 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     5101 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     5161 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     5221 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     5281 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     5341 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     5401 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     5461 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     5521 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     5581 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     5641 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     5701 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     5761 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     5821 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     5881 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     5941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6601 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6661 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6721 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6781 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6841 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     6901 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     6961 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     7021 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     7081 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     7141 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
     7201 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
     7261 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     7321 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgc
     7381 accaggcttt acactttatg cttccggctc gatgcagttt aaggtttaca cctataaaag
     7441 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
     7501 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtcc cccgtgaact
     7561 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     7621 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     7681 caaaaacgcc attaacctga tgttctgggg aataatgtca ggctccctta tacacagcca
     7741 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     7801 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcggttca
     7861 gctttcttgt acaaagtggt catccgcggt ctagagggcc cgtttaaacc cgctgatcag
     7921 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     7981 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8041 attgtctgag taggtgtcat tctattctgg ggggccgggt ggtgctggcc ctggccaggg
     8101 ccagcaccac ccggccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8521 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9241 ggcgagttac atgatcccct atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9841 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     9901 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     9961 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    10021 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    10081 tacgcgtt
//
