LOCUS       sequence-416284-        7867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coexpression of Cas9 and H2B mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7867)
  TITLE     Cas9-T2A-H2B mCitirine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Cas9-T2A-H2B mCitirine
FEATURES             Location/Qualifiers
     source          1..7867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..162
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             169..189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             214..4314
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             214..4314
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4315..4362
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4372..4425
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4426..4803
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4825..4834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4831..4833,4834..4836,4837..5547)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5137..5156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5484..5505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5571..5595)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5624..5745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5635..5654)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5689..5708
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5861..5878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6032..6620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6112..6131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6791..7582,7583..7651))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7414..7433
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7652..7756)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7824..7842
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caactttgta tagaaaagtt gggatccttg actagtgcgt taattaatgc gatcgcatcg
       61 atacaagttt gtacaaaaaa gcaggctgcc accatggact ataaggacca cgacggagac
      121 tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc aaagaagaag
      181 cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggac
      241 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
      301 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
      361 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
      421 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
      481 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
      541 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
      601 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
      661 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
      721 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
      781 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
      841 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
      901 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
      961 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
     1021 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
     1081 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
     1141 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
     1201 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
     1261 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
     1321 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
     1381 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
     1441 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
     1501 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
     1561 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
     1621 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
     1681 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
     1741 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
     1801 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
     1861 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
     1921 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
     1981 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
     2041 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
     2101 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
     2161 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
     2221 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
     2281 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
     2341 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
     2401 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
     2461 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
     2521 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
     2581 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
     2641 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
     2701 aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac
     2761 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
     2821 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
     2881 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
     2941 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
     3001 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
     3061 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
     3121 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     3181 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac
     3241 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
     3301 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
     3361 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
     3421 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
     3481 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
     3541 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
     3601 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
     3661 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
     3721 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
     3781 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
     3841 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
     3901 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
     3961 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
     4021 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
     4081 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
     4141 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
     4201 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
     4261 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg cgacaaaagg
     4321 ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggaagcgg agagggcagg
     4381 ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gccccatgcc agagccagcg
     4441 aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa
     4501 ggcggcaaga agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt
     4561 ctgaagcagg tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg
     4621 tttgtgaacg acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac
     4681 aagcgctcga ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg
     4741 gagttggcca agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct
     4801 aaggatcacc ggtcgccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     4861 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4921 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4981 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg
     5041 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     5101 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     5161 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5221 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     5281 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     5341 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5401 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     5461 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5521 actctcggca tggacgagct gtacaagtaa tgctatctag atcgaggcct acccagcttt
     5581 cttgtacaaa gtggtgatgg ccggccgctt cgagcagaca tgataagata cattgatgag
     5641 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5701 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     5761 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     5821 ctctacaaat gtggtagcgg ccgcggcgct cttccgcttc ctcgctcact gactcgctgc
     5881 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5941 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6001 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6061 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6121 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6181 gatacctgtc cgcctttctc tcttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6241 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6301 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6361 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6421 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6481 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6541 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6601 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6661 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6721 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6781 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6841 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6901 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6961 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7021 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7081 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7141 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7201 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7261 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7321 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7381 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7441 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7501 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7561 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7621 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7681 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7741 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7801 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cggcgcgccg
     7861 cggccgc
//
