LOCUS       sequence-416285-       12419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock promoter - activated hyperdCas12a-based transcriptional 
            base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Liu P, Foiret J, Situ Y, Zhang N, Kare AJ, Wu B, Raie MN, Ferrara 
            KW, Qi LS
  TITLE     Sonogenetic control of multiplexed genome regulation and base 
            editing.
  JOURNAL   Nat Commun. 2023 Oct 18;14(1):6575. doi: 10.1038/s41467-023-42249-8.
  PUBMED    37852951
REFERENCE   2  (bases 1 to 12419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-18"; volume: "14(1):6575. doi"; pages: " 
            10.1038/s41467-023-42249-8"
COMMENT     Sequence Label: pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro
FEATURES             Location/Qualifiers
     source          1..12419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     449..469
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(572..605)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    661..1249
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(714..734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1132..1143)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1252..1268)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1253..1269)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1778..1958
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2020..2608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2100..2119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2779..3570,3571..3639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3402..3421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3640..3744)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3819..3838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4010..4389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4391..4589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4544..4564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4607..4787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4834..4959
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5456..5689
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5874..5918
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6216..6333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             7790..7810
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8405..12085
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             12086..12133
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     promoter        12150..12361
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             join(12372..12419,1..552)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12372..12391)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
       61 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
      121 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
      181 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
      241 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
      301 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tttctccaga ccaccagggc
      361 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
      421 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
      481 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
      541 cccggtgcct gagaattcgt cgagggacct aataacttcg tatagcatac attatacgaa
      601 gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat
      661 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
      721 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
      781 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
      841 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
      901 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
      961 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     1021 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     1081 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     1141 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     1201 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     1261 cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     1321 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     1381 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     1441 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     1501 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     1561 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     1621 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     1681 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     1741 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     1801 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1861 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1921 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca
     1981 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2041 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2101 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2161 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2221 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2281 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2341 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2401 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2461 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2521 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     3781 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     3841 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     3901 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     3961 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     4021 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     4081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     4141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     4201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     4261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     4321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     4441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     4501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     4561 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     4621 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4681 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4741 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     4801 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     4861 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     4921 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     4981 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     5041 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     5101 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     5161 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     5221 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     5281 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     5341 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     5401 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     5461 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     5521 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     5581 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     5641 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     5701 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     5761 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     5821 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     5881 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     5941 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     6001 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     6061 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     6121 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     6181 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     6241 gggtacagag caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     6301 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     6361 ccagtttggt tagtaccggg cccgctctag agatctgaat ggaatgttct ggattgaaga
     6421 aagtgggaaa tggcctcaat tcacaaagtc acaacctgat aaaaaccagt gtgactttac
     6481 tgcccagtga acccatctcg tcctccagcc tttaggaggt aggttggact ggagcctgca
     6541 gtagtttact ctccacctga gtcctggtct ccagctggga acccacttag gccatagaga
     6601 aaaacgcaca ctgtgcctct ccaccgggcc tctggagacg aggctcctcg gggatacaaa
     6661 cagtggggag aacatgaggg acatcccgac cgtactctgc gtcctccttt cccaggtgtt
     6721 gcgttctgtc ttgggctgag tggcgaggtc tctcccgagt cccagggcca cagtgcaatg
     6781 tcacatctcc tttgtggaaa gtgactggta aaggagagag aacaaaactg gaggaacgta
     6841 aagtcttcag ccacctggtt taatttattc aagagtgatt aatcctagat gagaaaaaga
     6901 attgaaatgg atcggaaaaa aaatgaaagt gcattggccg ggaatcgaac ccgggcctcc
     6961 cgcgtggcag gcgagaattc taccactgaa ccaccaatgc tactgtcagc taaagacctg
     7021 cagtattgtc tcttaaagct cactatctct ggccattcac taaggaacca ggcaccgtct
     7081 taaatcgcgg tttggaaaat attttgttca agataaaact gttttaagat atacgtgtat
     7141 atatcttata tatctgtatt cgcatggtaa catatcttcg gcctccctga gccgctgggc
     7201 tctcagcggc cctccaaggc agcccgcagg cccctgtgtg cctcagggat ccgacctccc
     7261 acagccccgg ggagaccttg cctctaaagt tgctgctttt gcagcctctg ccacaaccgc
     7321 gcgtcctcag agccagcccg gaggagctag aaccttcccc gcatttcttt cagcagcctg
     7381 agtcagaggc gggctggcct ggcgtagccg cccagcctcg cggctcatgc cccgatctgc
     7441 ccgaaccttc tcccggggtc agcgccgcgc cgcgccaccc ggctgagtca gcccgggcgg
     7501 gcgagaggct ctcaactggg cgggaaggtg cgggaaggtg cggaaaggtt cgcgaaagtt
     7561 cgcggcggcg ggggtcgggt gaggcgcaaa aggataaaaa gcccgtggaa gcggagctga
     7621 gcagatccga gccgggctgg ctgcagagaa accgcaggga gagcctcact gctgagcgcc
     7681 cctcgacggc ggagcggcag cagcctccgt ggcctccagc atccgacaag aagcttcagc
     7741 cgttaacgcc accatgaaac ggacagccga cggaagcgag ttcgagtcac caaagaagaa
     7801 gcggaaagtc tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct
     7861 ggccaagagg gcacgggatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa
     7921 tagagtgatc ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc
     7981 cgaaattatg gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc
     8041 caccctgtac gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag
     8101 gatcggccgc gtggtgtttg gcgtgaggaa ctcaaaaaga ggcgccgcag gctccctgat
     8161 gaacgtgctg aactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc
     8221 agatgaatgt gccgccctgc tgtgcgattt ctatcggatg cctagacagg tgttcaatgc
     8281 tcagaagaag gcccagagct ccatcaactc tggaggatct agcggaggat cctctggcag
     8341 cgagacacca ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggcgg
     8401 cagcagcaag ctggagaagt ttacaaactg ctactccctg tctaagaccc tgaggttcaa
     8461 ggccatccct gtgggcaaga cccaggagaa catcgacaat aagcggctgc tggtggagga
     8521 cgagaagaga gccgaggatt ataagggcgt gaagaagctg ctggatcgct actatctgtc
     8581 ttttatcaac gacgtgctgc acagcatcaa gctgaagaat ctgaacaatt acatcagcct
     8641 gttccggaag aaaaccagaa ccgagaagga gaataaggag ctggagaacc tggagatcaa
     8701 tctgcggaag gagatcgcca aggccttcaa gggcaacgag ggctacaagt ccctgtttaa
     8761 gaaggatatc atcgagacaa tcctgccaga gttcctggac gataaggacg agatcgccct
     8821 ggtgaacagc ttcaatggct ttaccacagc cttcaccggc ttctttcgga acagagagaa
     8881 tatgttttcc gaggaggcca agagcacatc catcgccttc aggtgtatca acgagaatct
     8941 gacccgctac atctctaata tggacatctt cgagaaggtg gacgccatct ttgataagca
     9001 cgaggtgcag gagatcaagg agaagatcct gaacagcgac tatgatgtgg aggatttctt
     9061 tgagggcgag ttctttaact ttgtgctgac acaggagggc atccgcgtgt ataacgccat
     9121 catcggcggc ttcgtgaccg agagcggcga gaagatcaag ggcctgaacg agtacatcaa
     9181 cctgtataat cagaaaacca agcagaagct gcctaagttt aagccactgt ataagcaggt
     9241 gctgagcgat cgggagtctc tgagcttcta cggccggggc tatacatccg atgaggaggt
     9301 gctggaggtg tttagaaaca ccctgaacaa gaacagcgag atcttcagct ccatcaagaa
     9361 gctggagaag ctgttcaaga attttgacga gtactctagc gccggcatct ttgtgaagaa
     9421 cggccccgcc atcagcacaa tctccaagcg tatcttcggc gagtggaacg tgatccggga
     9481 caagtggaat gccgagtatg acgatatcca cctgaagaag aaggccgtgg tgaccgagaa
     9541 gtacgaggac gatcggagaa agtccttcaa gaagatcggc tccttttctc tggagcagct
     9601 gcaggagtac gccgacgccg atctgtctgt ggtggagaag ctgaaggaga tcatcatcca
     9661 gaaggtggat gagatctaca aggtgtatgg ctcctctgag aagctgttcg acgccgattt
     9721 tgtgctggag aagagcctga agaagaacga cgccgtggtg gccatcatga aggacctgct
     9781 ggattctgtg aagagcttcg agaattacat caaggccttc tttggcgagg gcaaggagac
     9841 aaacagggac gagtccttct atggcgattt tgtgctggcc tacgacatcc tgctgaaggt
     9901 ggaccacatc tacgatgcca tccgcaatta tgtgacccag aagccctact ctaaggataa
     9961 gttcaagctg tattttcaga accctcagtt catgggcggc tgggacaagg ataaggagac
    10021 agactatcgg gccaccatcc tgagatacgg ctccaagtac tatctggcca tcatggataa
    10081 gaagtacgcc aagtgcctgc agaagatcga caaggacgat gtgaacggca attacgagaa
    10141 gatcaactat aagctgctgc ccggccctaa taagatgctg ccaaaggtgt tcttttctaa
    10201 gaagtggatg gcctactata accccagcga ggacatccag aagatctaca agaatggcac
    10261 attcaagaag ggcgatatgt ttaacctgaa tgactgtcac aagctgatcg acttctttaa
    10321 ggatagcatc tcccggtatc caaagtggtc caatgcctac gatttcaact tttctgagac
    10381 agagaagtat aaggacatcg ccggctttta cagagaggtg gaggagcagg gctataaggt
    10441 gagcttcgag tctgccagca agaaggaggt ggataagctg gtggaggagg gcaagctgta
    10501 tatgttccag atctataaca aggacttttc cgataagtct cacggcacac ccaatctgca
    10561 caccatgtac ttcaagctgc tgtttgacga gaacaatcac ggacagatca ggctgagcgg
    10621 aggagcagag ctgttcatga ggcgcgcctc cctgaagaag gaggagctgg tggtgcaccc
    10681 agccaactcc cctatcgcca acaagaatcc agataatccc aagaaaacca caaccctgtc
    10741 ctacgacgtg tataaggata agaggttttc tgaggaccag tacgagctgc acatcccaat
    10801 cgccatcaat aagtgcccca agaacatctt caagatcaat acagaggtgc gcgtgctgct
    10861 gaagcacgac gataacccct atgtgatcgg catcgccagg ggcgagcgca atctgctgta
    10921 tatcgtggtg gtggacggca agggcaacat cgtggagcag tattccctga acgagatcat
    10981 caacaacttc aacggcatca ggatcaagac agattaccac tctctgctgg acaagaagga
    11041 gaaggagagg ttcgaggccc gccagaactg gacctccatc gagaatatca aggagctgaa
    11101 ggccggctat atctctcagg tggtgcacaa gatctgcgag ctggtggaga agtacgatgc
    11161 cgtgatcgcc ctggaggacc tgaactctgg ctttaagaat agccgcgtga aggtggagaa
    11221 gcaggtgtat cagaagttcg agaagatgct gatcgataag ctgaactaca tggtggacaa
    11281 gaagtctaat ccttgtgcaa caggcggcgc cctgaagggc tatcagatca ccaataagtt
    11341 cgagagcttt aagtccatgt ctacccagaa cggcttcatc ttttacatcc ctgcctggct
    11401 gacatccaag atcgatccat ctaccggctt tgtgaacctg ctgaaaacca agtataccag
    11461 catcgccgat tccaagaagt tcatcagctc ctttgacagg atcatgtacg tgcccgagga
    11521 ggatctgttc gagtttgccc tggactataa gaacttctct cgcacagacg ccgattacat
    11581 caagaagtgg aagctgtact cctacggcaa ccggatcaga atcttccgga atcctaagaa
    11641 gaacaacgtg ttcgactggg aggaggtgtg cctgaccagc gcctataagg agctgttcaa
    11701 caagtacggc atcaattatc agcagggcga tatcagagcc ctgctgtgcg agcagtccga
    11761 caaggccttc tactctagct ttatggccct gatgagcctg atgctgcaga tgcggaacag
    11821 catcacaggc cgcaccgacg tggattttct gatcagccct gtgaagaact ccgacggcat
    11881 cttctacgat agccggaact atgaggccca ggagaatgcc atcctgccaa agaacgccga
    11941 cgccaatggc gcctataaca tcgccagaaa ggtgctgtgg gccatcggcc agttcaagaa
    12001 ggccgaggac gagaagctgg ataaggtgaa gatcgccatc tctaacaagg agtggctgga
    12061 gtacgcccag accagcgtga agcacaaaag gccggcggcc acgaaaaagg ccggccaggc
    12121 aaaaaagaaa aagtgagcgg ccgctcgacg ggcagagcgc acatcgccca cagtccccga
    12181 gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa
    12241 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
    12301 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
    12361 ggaccggtac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtc
//
