LOCUS       sequence-416286-       13578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock promoter - activated hyperdCas12a-based transcriptional 
            activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13578)
  AUTHORS   Liu P, Foiret J, Situ Y, Zhang N, Kare AJ, Wu B, Raie MN, Ferrara 
            KW, Qi LS
  TITLE     Sonogenetic control of multiplexed genome regulation and base 
            editing.
  JOURNAL   Nat Commun. 2023 Oct 18;14(1):6575. doi: 10.1038/s41467-023-42249-8.
  PUBMED    37852951
REFERENCE   2  (bases 1 to 13578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-18"; volume: "14(1):6575. doi"; pages: " 
            10.1038/s41467-023-42249-8"
COMMENT     Sequence Label: 
            pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry
FEATURES             Location/Qualifiers
     source          1..13578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    217..334
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        393..641
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     393..413
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     564..583
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    664..682
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             2061..5744
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5751..5777
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5757..5783
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5796..5816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5823..5843
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5880..6029
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6036..6056
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6189..6545
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     enhancer        6874..7177
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7178..7381
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             7424..8131
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7566..7584)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7834..7853
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(8151..8184)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8240..8828
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8293..8313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8711..8722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8831..8847)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8832..8848)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9357..9537
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9599..10187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9679..9698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10358..11149,11150..11218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10981..11000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11219..11323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11398..11417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11589..11968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11970..12168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             12186..12366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12413..12538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13035..13268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13453..13497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
       61 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
      121 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
      181 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
      241 ggggtacaga gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      301 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      361 tccagtttgg ttagtaccgg gcccgctcta gagagggcct atttcccatg attccttcat
      421 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
      481 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
      541 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
      601 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgaatttcta ctaagtgtag
      661 atctccctat cagtgataga gaacgttttt ttctcgagga tctgaatgga atgttctgga
      721 ttgaagaaag tgggaaatgg cctcaattca caaagtcaca acctgataaa aaccagtgtg
      781 actttactgc ccagtgaacc catctcgtcc tccagccttt aggaggtagg ttggactgga
      841 gcctgcagta gtttactctc cacctgagtc ctggtctcca gctgggaacc cacttaggcc
      901 atagagaaaa acgcacactg tgcctctcca ccgggcctct ggagacgagg ctcctcgggg
      961 atacaaacag tggggagaac atgagggaca tcccgaccgt actctgcgtc ctcctttccc
     1021 aggtgttgcg ttctgtcttg ggctgagtgg cgaggtctct cccgagtccc agggccacag
     1081 tgcaatgtca catctccttt gtggaaagtg actggtaaag gagagagaac aaaactggag
     1141 gaacgtaaag tcttcagcca cctggtttaa tttattcaag agtgattaat cctagatgag
     1201 aaaaagaatt gaaatggatc ggaaaaaaaa tgaaagtgca ttggccggga atcgaacccg
     1261 ggcctcccgc gtggcaggcg agaattctac cactgaacca ccaatgctac tgtcagctaa
     1321 agacctgcag tattgtctct taaagctcac tatctctggc cattcactaa ggaaccaggc
     1381 accgtcttaa atcgcggttt ggaaaatatt ttgttcaaga taaaactgtt ttaagatata
     1441 cgtgtatata tcttatatat ctgtattcgc atggtaacat atcttcggcc tccctgagcc
     1501 gctgggctct cagcggccct ccaaggcagc ccgcaggccc ctgtgtgcct cagggatccg
     1561 acctcccaca gccccgggga gaccttgcct ctaaagttgc tgcttttgca gcctctgcca
     1621 caaccgcgcg tcctcagagc cagcccggag gagctagaac cttccccgca tttctttcag
     1681 cagcctgagt cagaggcggg ctggcctggc gtagccgccc agcctcgcgg ctcatgcccc
     1741 gatctgcccg aaccttctcc cggggtcagc gccgcgccgc gccacccggc tgagtcagcc
     1801 cgggcgggcg agaggctctc aactgggcgg gaaggtgcgg gaaggtgcgg aaaggttcgc
     1861 gaaagttcgc ggcggcgggg gtcgggtgag gcgcaaaagg ataaaaagcc cgtggaagcg
     1921 gagctgagca gatccgagcc gggctggctg cagagaaacc gcagggagag cctcactgct
     1981 gagcgcccct cgacggcgga gcggcagcag cctccgtggc ctccagcatc cgacaagaag
     2041 cttcagccgt taacgccacc atgagcaagc tggagaagtt tacaaactgc tactccctgt
     2101 ctaagaccct gaggttcaag gccatccctg tgggcaagac ccaggagaac atcgacaata
     2161 agcggctgct ggtggaggac gagaagagag ccgaggatta taagggcgtg aagaagctgc
     2221 tggatcgcta ctatctgtct tttatcaacg acgtgctgca cagcatcaag ctgaagaatc
     2281 tgaacaatta catcagcctg ttccggaaga aaaccagaac cgagaaggag aataaggagc
     2341 tggagaacct ggagatcaat ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg
     2401 gctacaagtc cctgtttaag aaggatatca tcgagacaat cctgccagag ttcctggacg
     2461 ataaggacga gatcgccctg gtgaacagct tcaatggctt taccacagcc ttcaccggct
     2521 tctttcgtaa cagagagaat atgttttccg aggaggccaa gagcacatcc atcgccttca
     2581 ggtgtatcaa cgagaatctg acccgctaca tctctaatat ggacatcttc gagaaggtgg
     2641 acgccatctt tgataagcac gaggtgcagg agatcaagga gaagatcctg aacagcgact
     2701 atgatgtgga ggatttcttt gagggcgagt tctttaactt tgtgctgaca caggagggca
     2761 tccgcgtgta taacgccatc atcggcggct tcgtgaccga gagcggcgag aagatcaagg
     2821 gcctgaacga gtacatcaac ctgtataatc agaaaaccaa gcagaagctg cctaagttta
     2881 agccactgta taagcaggtg ctgagcgatc gggagtctct gagcttctac ggcaggggct
     2941 atacatccga tgaggaggtg ctggaggtgt ttagaaacac cctgaacaag aacagcgaga
     3001 tcttcagctc catcaagaag ctggagaagc tgttcaagaa ttttgacgag tactctagcg
     3061 ccggcatctt tgtgaagaac ggccccgcca tcagcacaat ctccaagcgt atcttcggcg
     3121 agtggaacgt gatccgggac aagtggaatg ccgagtatga cgatatccac ctgaagaaga
     3181 aggccgtggt gaccgagaag tacgaggacg atcggagaaa gtccttcaag aagatcggct
     3241 ccttttctct ggagcagctg caggagtacg ccgacgccga tctgtctgtg gtggagaagc
     3301 tgaaggagat catcatccag aaggtggatg agatctacaa ggtgtatggc tcctctgaga
     3361 agctgttcga cgccgatttt gtgctggaga agagcctgaa gaagaacgac gccgtggtgg
     3421 ccatcatgaa ggacctgctg gattctgtga agagcttcga gaattacatc aaggccttct
     3481 ttggcgaggg caaggagaca aacagggacg agtccttcta tggcgatttt gtgctggcct
     3541 acgacatcct gctgaaggtg gaccacatct acgatgccat ccgcaattat gtgacccaga
     3601 agccctactc taaggataag ttcaagctgt attttcagaa ccctcagttc atgggcggct
     3661 gggacaagga taaggagaca gactatcggg ccaccatcct gagatacggc tccaagtact
     3721 atctggccat catggataag aagtacgcca agtgcctgca gaagatcgac aaggacgatg
     3781 tgaacggcaa ttacgagaag atcaactata agctgctgcc cggccctaat aagatgctgc
     3841 caaaggtgtt cttttctaag aagtggatgg cctactataa ccccagcgag gacatccaga
     3901 agatctacaa gaatggcaca ttcaagaagg gcgatatgtt taacctgaat gactgtcaca
     3961 agctgatcga cttctttaag gatagcatct cccggtatcc aaagtggtcc aatgcctacg
     4021 atttcaactt ttctgagaca gagaagtata aggacatcgc cggcttttac agagaggtgg
     4081 aggagcaggg ctataaggtg agcttcgagt ctgccagcaa gaaggaggtg gataagctgg
     4141 tggaggaggg caagctgtat atgttccaga tctataacaa ggacttttcc gataagtctc
     4201 acggcacacc caatctgcac accatgtact tcaagctgct gtttgacgag aacaatcacg
     4261 gacagatcag gctgagcgga ggagcagagc tgttcatgag gcgcgcctcc ctgaagaagg
     4321 aggagctggt ggtgcaccca gccaactccc ctatcgccaa caagaatcca gataatccca
     4381 agaaaaccac aaccctgtcc tacgacgtgt ataaggataa gaggttttct gaggaccagt
     4441 acgagctgca catcccaatc gccatcaata agtgccccaa gaacatcttc aagatcaata
     4501 cagaggtgcg cgtgctgctg aagcacgacg ataaccccta tgtgatcggc atcgccaggg
     4561 gcgagcgcaa tctgctgtat atcgtggtgg tggacggcaa gggcaacatc gtggagcagt
     4621 attccctgaa cgagatcatc aacaacttca acggcatcag gatcaagaca gattaccact
     4681 ctctgctgga caagaaggag aaggagaggt tcgaggcccg ccagaactgg acctccatcg
     4741 agaatatcaa ggagctgaag gccggctata tctctcaggt ggtgcacaag atctgcgagc
     4801 tggtggagaa gtacgatgcc gtgatcgccc tggaggacct gaactctggc tttaagaata
     4861 gccgcgtgaa ggtggagaag caggtgtatc agaagttcga gaagatgctg atcgataagc
     4921 tgaactacat ggtggacaag aagtctaatc cttgtgcaac aggcggcgcc ctgaagggct
     4981 atcagatcac caataagttc gagagcttta agtccatgtc tacccagaac ggcttcatct
     5041 tttacatccc tgcctggctg acatccaaga tcgatccatc taccggcttt gtgaacctgc
     5101 tgaaaaccaa gtataccagc atcgccgatt ccaagaagtt catcagctcc tttgacagga
     5161 tcatgtacgt gcccgaggag gatctgttcg agtttgccct ggactataag aacttctctc
     5221 gcacagacgc cgattacatc aagaagtgga agctgtactc ctacggcaac cggatcagaa
     5281 tcttccggaa tcctaagaag aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg
     5341 cctataagga gctgttcaac aagtacggca tcaattatca gcagggcgat atcagagccc
     5401 tgctgtgcga gcagtccgac aaggccttct actctagctt tatggccctg atgagcctga
     5461 tgctgcagat gcggaacagc atcacaggcc gcaccgacgt ggattttctg atcagccctg
     5521 tgaagaactc cgacggcatc ttctacgata gccggaacta tgaggcccag gagaatgcca
     5581 tcctgccaaa gaacgccgac gccaatggcg cctataacat cgccagaaag gtgctgtggg
     5641 ccatcggcca gttcaagaag gccgaggacg agaagctgga taaggtgaag atcgccatct
     5701 ctaacaagga gtggctggag tacgcccaga ccagcgtgaa gcacggatcc tatccctatg
     5761 acgtgcccga ttatgccagc ctgggcagcg gctcccccaa gaaaaaacgc aaggtggaag
     5821 atcctaagaa aaagcggaaa gtggacggca ttggtagtgg gagcaacggc agcagcctcg
     5881 atgctttaga cgattttgac ttagatatgc ttggttcaga cgcgttagac gacttcgacc
     5941 tagacatgtt aggctcagat gcattggacg acttcgattt agatatgttg ggctccgatg
     6001 ccctagatga ctttgatcta gatatgctag gtagtcccaa aaagaagagg aaagtgggat
     6061 ccgtccttcc gcaagcgcct gccccagcgc ctgcaccggc aatggtgtcc gccctcgcac
     6121 aggcccctgc gcccgtcccc gtgctcgcgc ctggaccgcc ccaggcggtc gctccaccgg
     6181 ctccgaagcc gacgcaggcc ggagagggaa cactctccga agcacttctt caactccagt
     6241 ttgatgacga ggatcttgga gcactccttg gaaactcgac agaccctgcg gtgtttaccg
     6301 acctcgcgtc agtagataac tccgaatttc agcagctttt gaaccagggt atcccggtcg
     6361 cgccacatac aacggagccc atgttgatgg aataccccga agcaatcacg agacttgtga
     6421 cgggagcgca gcggcctccc gatcccgcac ccgcaccttt gggggcacct ggcctcccta
     6481 acggactttt gagcggcgac gaggatttct cctccatcgc cgatatggat ttctcagcct
     6541 tgctgtcagg ctctggcagc ggcagcgacc tttcccatcc gcccccaagg ggccatctgg
     6601 atgagctgac aaccacactt gagtccatga ccgaggatct gaacctggac tcacccctga
     6661 ccccggaatt gaacgagatt ctggatacct tcctgaacga cgagtgcctc ttgcatgcca
     6721 tgcatatcag cacaggactg tccatcttcg acacatctct gttttgagga tccattaggc
     6781 ggccgcgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     6841 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     6901 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     6961 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     7021 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     7081 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     7141 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     7201 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     7261 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     7321 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     7381 tggtttagtg aaccgtcaga tccgctagcc caccatgccc ggggtgagca agggcgagga
     7441 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     7501 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     7561 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     7621 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     7681 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     7741 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     7801 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     7861 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     7921 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     7981 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     8041 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     8101 caccggcggc atggacgagc tgtacaagta agaattcgtc gagggaccta ataacttcgt
     8161 atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt
     8221 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8281 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8341 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8401 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8461 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8521 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8581 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     8641 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8701 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8761 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8821 ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa
     8881 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     8941 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     9001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9061 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     9121 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     9181 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     9241 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     9301 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     9361 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9421 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9481 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     9541 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9601 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9661 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9721 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9781 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9841 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9901 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9961 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10021 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10081 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10141 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10201 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10261 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10321 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10381 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10441 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10501 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10561 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10621 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10681 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10741 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10801 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10861 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10921 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10981 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11041 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11101 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11161 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11221 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11281 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11341 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
    11401 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
    11461 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
    11521 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
    11581 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
    11641 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
    11701 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
    11761 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
    11821 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
    11881 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
    11941 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
    12001 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
    12061 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
    12121 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct
    12181 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    12241 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    12301 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    12361 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
    12421 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
    12481 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
    12541 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
    12601 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
    12661 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
    12721 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
    12781 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
    12841 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
    12901 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    12961 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    13021 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    13081 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    13141 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    13201 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    13261 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    13321 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    13381 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    13441 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    13501 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    13561 ggcttggtag gtttaaga
//
