LOCUS       sequence-416287-       15331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock promoter - activated hyperdCas12a-based transcriptional 
            activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15331)
  AUTHORS   Liu P, Foiret J, Situ Y, Zhang N, Kare AJ, Wu B, Raie MN, Ferrara 
            KW, Qi LS
  TITLE     Sonogenetic control of multiplexed genome regulation and base 
            editing.
  JOURNAL   Nat Commun. 2023 Oct 18;14(1):6575. doi: 10.1038/s41467-023-42249-8.
  PUBMED    37852951
REFERENCE   2  (bases 1 to 15331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-18"; volume: "14(1):6575. doi"; pages: " 
            10.1038/s41467-023-42249-8"
COMMENT     Sequence Label: pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro
FEATURES             Location/Qualifiers
     source          1..15331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    91..216
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    713..946
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1131..1175
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1460..1577
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3000..6683
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             6690..6716
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6696..6722
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             6735..6755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6762..6782
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6819..6968
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6975..6995
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7128..7484
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     promoter        7720..7931
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             7942..8541
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7942..7961)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8438..8458
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8605..9193
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8658..8678)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9076..9087)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9196..9212)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9197..9213)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9303..9536
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9561..9579)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9562..9579)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9869..9888)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10102..10121)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10221..10243
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10281..10299)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10367..10471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10472..10540,10541..11332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10690..10709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11503..12091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11992..12011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12245..12262
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12332..12352)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12337..12666
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12517..12652
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13800..13865
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13995..14015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14440..14574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14477..14496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14531..14550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(14742..15331,1..44)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
       61 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      121 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      181 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      241 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
      301 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
      361 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
      421 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
      481 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
      541 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      601 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
      661 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
      721 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
      781 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
      841 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
      901 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
      961 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1021 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1081 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1141 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1201 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1261 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1321 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1381 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa
     1441 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     1501 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     1561 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata
     1621 agcttgatat cgaattcgat ctgaatggaa tgttctggat tgaagaaagt gggaaatggc
     1681 ctcaattcac aaagtcacaa cctgataaaa accagtgtga ctttactgcc cagtgaaccc
     1741 atctcgtcct ccagccttta ggaggtaggt tggactggag cctgcagtag tttactctcc
     1801 acctgagtcc tggtctccag ctgggaaccc acttaggcca tagagaaaaa cgcacactgt
     1861 gcctctccac cgggcctctg gagacgaggc tcctcgggga tacaaacagt ggggagaaca
     1921 tgagggacat cccgaccgta ctctgcgtcc tcctttccca ggtgttgcgt tctgtcttgg
     1981 gctgagtggc gaggtctctc ccgagtccca gggccacagt gcaatgtcac atctcctttg
     2041 tggaaagtga ctggtaaagg agagagaaca aaactggagg aacgtaaagt cttcagccac
     2101 ctggtttaat ttattcaaga gtgattaatc ctagatgaga aaaagaattg aaatggatcg
     2161 gaaaaaaaat gaaagtgcat tggccgggaa tcgaacccgg gcctcccgcg tggcaggcga
     2221 gaattctacc actgaaccac caatgctact gtcagctaaa gacctgcagt attgtctctt
     2281 aaagctcact atctctggcc attcactaag gaaccaggca ccgtcttaaa tcgcggtttg
     2341 gaaaatattt tgttcaagat aaaactgttt taagatatac gtgtatatat cttatatatc
     2401 tgtattcgca tggtaacata tcttcggcct ccctgagccg ctgggctctc agcggccctc
     2461 caaggcagcc cgcaggcccc tgtgtgcctc agggatccga cctcccacag ccccggggag
     2521 accttgcctc taaagttgct gcttttgcag cctctgccac aaccgcgcgt cctcagagcc
     2581 agcccggagg agctagaacc ttccccgcat ttctttcagc agcctgagtc agaggcgggc
     2641 tggcctggcg tagccgccca gcctcgcggc tcatgccccg atctgcccga accttctccc
     2701 ggggtcagcg ccgcgccgcg ccacccggct gagtcagccc gggcgggcga gaggctctca
     2761 actgggcggg aaggtgcggg aaggtgcgga aaggttcgcg aaagttcgcg gcggcggggg
     2821 tcgggtgagg cgcaaaagga taaaaagccc gtggaagcgg agctgagcag atccgagccg
     2881 ggctggctgc agagaaaccg cagggagagc ctcactgctg agcgcccctc gacggcggag
     2941 cggcagcagc ctccgtggcc tccagcatcc gacaagaagc ttcagccgtt aacgccacca
     3001 tgagcaagct ggagaagttt acaaactgct actccctgtc taagaccctg aggttcaagg
     3061 ccatccctgt gggcaagacc caggagaaca tcgacaataa gcggctgctg gtggaggacg
     3121 agaagagagc cgaggattat aagggcgtga agaagctgct ggatcgctac tatctgtctt
     3181 ttatcaacga cgtgctgcac agcatcaagc tgaagaatct gaacaattac atcagcctgt
     3241 tccggaagaa aaccagaacc gagaaggaga ataaggagct ggagaacctg gagatcaatc
     3301 tgcggaagga gatcgccaag gccttcaagg gcaacgaggg ctacaagtcc ctgtttaaga
     3361 aggatatcat cgagacaatc ctgccagagt tcctggacga taaggacgag atcgccctgg
     3421 tgaacagctt caatggcttt accacagcct tcaccggctt ctttcgtaac agagagaata
     3481 tgttttccga ggaggccaag agcacatcca tcgccttcag gtgtatcaac gagaatctga
     3541 cccgctacat ctctaatatg gacatcttcg agaaggtgga cgccatcttt gataagcacg
     3601 aggtgcagga gatcaaggag aagatcctga acagcgacta tgatgtggag gatttctttg
     3661 agggcgagtt ctttaacttt gtgctgacac aggagggcat ccgcgtgtat aacgccatca
     3721 tcggcggctt cgtgaccgag agcggcgaga agatcaaggg cctgaacgag tacatcaacc
     3781 tgtataatca gaaaaccaag cagaagctgc ctaagtttaa gccactgtat aagcaggtgc
     3841 tgagcgatcg ggagtctctg agcttctacg gcaggggcta tacatccgat gaggaggtgc
     3901 tggaggtgtt tagaaacacc ctgaacaaga acagcgagat cttcagctcc atcaagaagc
     3961 tggagaagct gttcaagaat tttgacgagt actctagcgc cggcatcttt gtgaagaacg
     4021 gccccgccat cagcacaatc tccaagcgta tcttcggcga gtggaacgtg atccgggaca
     4081 agtggaatgc cgagtatgac gatatccacc tgaagaagaa ggccgtggtg accgagaagt
     4141 acgaggacga tcggagaaag tccttcaaga agatcggctc cttttctctg gagcagctgc
     4201 aggagtacgc cgacgccgat ctgtctgtgg tggagaagct gaaggagatc atcatccaga
     4261 aggtggatga gatctacaag gtgtatggct cctctgagaa gctgttcgac gccgattttg
     4321 tgctggagaa gagcctgaag aagaacgacg ccgtggtggc catcatgaag gacctgctgg
     4381 attctgtgaa gagcttcgag aattacatca aggccttctt tggcgagggc aaggagacaa
     4441 acagggacga gtccttctat ggcgattttg tgctggccta cgacatcctg ctgaaggtgg
     4501 accacatcta cgatgccatc cgcaattatg tgacccagaa gccctactct aaggataagt
     4561 tcaagctgta ttttcagaac cctcagttca tgggcggctg ggacaaggat aaggagacag
     4621 actatcgggc caccatcctg agatacggct ccaagtacta tctggccatc atggataaga
     4681 agtacgccaa gtgcctgcag aagatcgaca aggacgatgt gaacggcaat tacgagaaga
     4741 tcaactataa gctgctgccc ggccctaata agatgctgcc aaaggtgttc ttttctaaga
     4801 agtggatggc ctactataac cccagcgagg acatccagaa gatctacaag aatggcacat
     4861 tcaagaaggg cgatatgttt aacctgaatg actgtcacaa gctgatcgac ttctttaagg
     4921 atagcatctc ccggtatcca aagtggtcca atgcctacga tttcaacttt tctgagacag
     4981 agaagtataa ggacatcgcc ggcttttaca gagaggtgga ggagcagggc tataaggtga
     5041 gcttcgagtc tgccagcaag aaggaggtgg ataagctggt ggaggagggc aagctgtata
     5101 tgttccagat ctataacaag gacttttccg ataagtctca cggcacaccc aatctgcaca
     5161 ccatgtactt caagctgctg tttgacgaga acaatcacgg acagatcagg ctgagcggag
     5221 gagcagagct gttcatgagg cgcgcctccc tgaagaagga ggagctggtg gtgcacccag
     5281 ccaactcccc tatcgccaac aagaatccag ataatcccaa gaaaaccaca accctgtcct
     5341 acgacgtgta taaggataag aggttttctg aggaccagta cgagctgcac atcccaatcg
     5401 ccatcaataa gtgccccaag aacatcttca agatcaatac agaggtgcgc gtgctgctga
     5461 agcacgacga taacccctat gtgatcggca tcgccagggg cgagcgcaat ctgctgtata
     5521 tcgtggtggt ggacggcaag ggcaacatcg tggagcagta ttccctgaac gagatcatca
     5581 acaacttcaa cggcatcagg atcaagacag attaccactc tctgctggac aagaaggaga
     5641 aggagaggtt cgaggcccgc cagaactgga cctccatcga gaatatcaag gagctgaagg
     5701 ccggctatat ctctcaggtg gtgcacaaga tctgcgagct ggtggagaag tacgatgccg
     5761 tgatcgccct ggaggacctg aactctggct ttaagaatag ccgcgtgaag gtggagaagc
     5821 aggtgtatca gaagttcgag aagatgctga tcgataagct gaactacatg gtggacaaga
     5881 agtctaatcc ttgtgcaaca ggcggcgccc tgaagggcta tcagatcacc aataagttcg
     5941 agagctttaa gtccatgtct acccagaacg gcttcatctt ttacatccct gcctggctga
     6001 catccaagat cgatccatct accggctttg tgaacctgct gaaaaccaag tataccagca
     6061 tcgccgattc caagaagttc atcagctcct ttgacaggat catgtacgtg cccgaggagg
     6121 atctgttcga gtttgccctg gactataaga acttctctcg cacagacgcc gattacatca
     6181 agaagtggaa gctgtactcc tacggcaacc ggatcagaat cttccggaat cctaagaaga
     6241 acaacgtgtt cgactgggag gaggtgtgcc tgaccagcgc ctataaggag ctgttcaaca
     6301 agtacggcat caattatcag cagggcgata tcagagccct gctgtgcgag cagtccgaca
     6361 aggccttcta ctctagcttt atggccctga tgagcctgat gctgcagatg cggaacagca
     6421 tcacaggccg caccgacgtg gattttctga tcagccctgt gaagaactcc gacggcatct
     6481 tctacgatag ccggaactat gaggcccagg agaatgccat cctgccaaag aacgccgacg
     6541 ccaatggcgc ctataacatc gccagaaagg tgctgtgggc catcggccag ttcaagaagg
     6601 ccgaggacga gaagctggat aaggtgaaga tcgccatctc taacaaggag tggctggagt
     6661 acgcccagac cagcgtgaag cacggatcct atccctatga cgtgcccgat tatgccagcc
     6721 tgggcagcgg ctcccccaag aaaaaacgca aggtggaaga tcctaagaaa aagcggaaag
     6781 tggacggcat tggtagtggg agcaacggca gcagcctcga tgctttagac gattttgact
     6841 tagatatgct tggttcagac gcgttagacg acttcgacct agacatgtta ggctcagatg
     6901 cattggacga cttcgattta gatatgttgg gctccgatgc cctagatgac tttgatctag
     6961 atatgctagg tagtcccaaa aagaagagga aagtgggatc cgtccttccg caagcgcctg
     7021 ccccagcgcc tgcaccggca atggtgtccg ccctcgcaca ggcccctgcg cccgtccccg
     7081 tgctcgcgcc tggaccgccc caggcggtcg ctccaccggc tccgaagccg acgcaggccg
     7141 gagagggaac actctccgaa gcacttcttc aactccagtt tgatgacgag gatcttggag
     7201 cactccttgg aaactcgaca gaccctgcgg tgtttaccga cctcgcgtca gtagataact
     7261 ccgaatttca gcagcttttg aaccagggta tcccggtcgc gccacataca acggagccca
     7321 tgttgatgga ataccccgaa gcaatcacga gacttgtgac gggagcgcag cggcctcccg
     7381 atcccgcacc cgcacctttg ggggcacctg gcctccctaa cggacttttg agcggcgacg
     7441 aggatttctc ctccatcgcc gatatggatt tctcagcctt gctgtcaggc tctggcagcg
     7501 gcagcgacct ttcccatccg cccccaaggg gccatctgga tgagctgaca accacacttg
     7561 agtccatgac cgaggatctg aacctggact cacccctgac cccggaattg aacgagattc
     7621 tggatacctt cctgaacgac gagtgcctct tgcatgccat gcatatcagc acaggactgt
     7681 ccatcttcga cacatctctg ttttgagcgg ccgctcgacg ggcagagcgc acatcgccca
     7741 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
     7801 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     7861 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     7921 ccagaacaca ggaccggtac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7981 gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     8041 cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     8101 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     8161 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8221 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8281 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ttctccagac
     8341 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8401 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     8461 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8521 acccgcaagc ccggtgccta aacgcgtggc cgcgactcta gagtcgacct gcaggcatgc
     8581 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     8641 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8701 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8761 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8821 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8881 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8941 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     9001 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9061 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9121 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9181 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     9241 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     9301 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     9361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     9541 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     9601 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     9661 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     9721 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     9781 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     9841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     9901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     9961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
    10021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
    10081 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
    10141 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
    10201 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
    10261 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    10321 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10381 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10441 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10501 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10561 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10621 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10681 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    10741 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    10801 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    10861 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    10921 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    10981 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    11041 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    11101 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    11161 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    11221 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    11281 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    11341 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    11401 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    11461 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    11521 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    11581 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    11641 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    11701 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    11761 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    11821 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    11881 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    11941 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    12001 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    12061 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    12121 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    12181 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    12241 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    12301 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
    12361 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    12421 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    12481 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    12541 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    12601 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    12661 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
    12721 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
    12781 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
    12841 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
    12901 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
    12961 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
    13021 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
    13081 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
    13141 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    13201 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
    13261 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    13321 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
    13381 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
    13441 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
    13501 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
    13561 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
    13621 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
    13681 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
    13741 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
    13801 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
    13861 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
    13921 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
    13981 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
    14041 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
    14101 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
    14161 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
    14221 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
    14281 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
    14341 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    14401 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    14461 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    14521 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    14581 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    14641 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    14701 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
    14761 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
    14821 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
    14881 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
    14941 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
    15001 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
    15061 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
    15121 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
    15181 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    15241 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    15301 gtgcccgtct gttgtgtgac tctggtaact a
//
