LOCUS       sequence-416303-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human TCF12 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Henriques-Oliveira L, Altman AR, Kurochkin I, Ascic E, Halitzki E, 
            Matei A, Pertiga-Cabral D, Ulmert I, Holst S, Nair MS, Cunha PP, 
            Park S, Vergani S, Kharas MG, Yuan J, Lahl K, Rosa FF, Pires CF, 
            Pereira C-F
  TITLE     Anchored screening identifies transcription factor blueprints 
            underlying dendritic cell diversity and subset-specific anti-tumor 
            immunity
  JOURNAL   Immunity. 58, 1-20. October 2025.
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            58, 1-20. October 2025."
COMMENT     Sequence Label: pFUW-TetO-TCF12
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2445..2463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2487..2505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2529..2547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2571..2589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2613..2631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2664..2702
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2699..2723
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4949..4965)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4950..4966)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5471..5651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5677..5694)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5683..5907
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5953..6381
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6040..6059)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6250..6271
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6390..6410)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6395..6724
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6575..6710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6637..6656
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6772..6819
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6838..7212
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7342..7463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7379..7398)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7433..7452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7512..7528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7512..7528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7525..7547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7536..7552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7560..7566,7567..7584,7585..7590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7605..7626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7743..7760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7914..8502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7994..8013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8673..9464,9465..9533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9296..9315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9534..9638)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9713..9732)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2701 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2761 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccggttct agagctagct
     2821 tcgaaatgaa tccccagcaa caacgcatgg ccgctatagg gaccgacaag gagctgagcg
     2881 acctactgga cttcagtgcg atgttttccc cacctgttaa tagtgggaaa actagaccaa
     2941 ctacactggg aagcagtcag ttcagtggat caggtattga tgaaagagga ggtacaacat
     3001 cttggggaac aagtggtcaa ccaagtcctt cctatgattc atctagaggt tttacagaca
     3061 gccctcatta cagtgatcac ttgaatgaca gtcgattagg agcccatgaa ggcttgtccc
     3121 caacaccttt catgaactca aatctgatgg gaaaaacatc agagagaggc tcattttccc
     3181 tgtacagcag agatactgga ttaccaggct gtcaatctag tctcctgaga caagatctgg
     3241 ggcttgggag cccagcacag ctatcttctt caggaaaacc tgggacagca tactattcat
     3301 tctctgctac aagttccagg aggagaccac tccatgactc tgcagcgctt gatcccttgc
     3361 aagcaaaaaa agtcagaaag gtgcctcctg gtttgccttc ttctgtatat gcaccatccc
     3421 caaattcaga tgatttcaac cgtgaatctc ctagttatcc atctcctaag ccaccaacca
     3481 gtatgttcgc tagcactttc tttatgcaag atgggaccca caattcttct gacctttgga
     3541 gttcatcaaa tgggatgagc cagcctggtt ttggtggaat tctggggacc tccacttccc
     3601 acatgtctca atccagtagt tatggcaacc ttcattcaca tgaccgcttg agttatcctc
     3661 cacactcagt ttcaccaaca gacataaaca cgagtcttcc accaatgtcc agctttcatc
     3721 gcggcagtac cagcagttca ccttacgttg ctgcctcaca cactcctccc atcaatggat
     3781 cagacagcat tctaggaacc agagggaatg ctgctggaag ctcacagaca ggtgatgcac
     3841 ttggaaaggc tttggcatct atttattctc ctgaccatac cagcagtagt tttccgtcaa
     3901 atccatcaac accagttgga tcaccttcac ctctcacagg taccagtcag tggccaagac
     3961 ctggagggca agcaccttca tccccaagct atgaaaactc actccactcc ctgaaaaatc
     4021 gagttgagca gcaacttcac gagcatttgc aagatgcaat gtccttctta aaggatgtct
     4081 gtgagcagtc tcgaatggag gatcgtttag acagactgga tgatgcaatc catgtgctgc
     4141 ggaaccatgc tgtgggacct tccaccagtt tgcctgctgg tcacagtgat atacatagtt
     4201 tattgggacc atcccataat gcaccaattg gaagcctcaa ttcaaactat ggaggatcaa
     4261 gccttgttgc aagcagtcga tcagcttcaa tggttggaac tcatcgggaa gactctgtca
     4321 gtctcaatgg caatcattca gtcctgtcta gtacagtcac tacttcaagc acagacctga
     4381 accataaaac acaagaaaat tatagaggtg gcttgcaaag tcagtctgga actgttgtta
     4441 caacagaaat caagactgaa aacaaagaaa aggatgaaaa ccttcatgaa cctccttcat
     4501 cagatgacat gaagtcagat gatgaatcct cccaaaaaga tatcaaggtt tcatctagag
     4561 gcagaacaag cagtactaat gaagatgagg atttgaaccc tgaacagaag atagaaaggg
     4621 agaaggagag gcggatggct aacaatgcca gagaacgctt acgcgtgcgg gatattaatg
     4681 aagcattcaa agagcttggc cgaatgtgtc agcttcactt gaagagtgaa aaaccccaaa
     4741 caaaactcct tattcttcat caagccgtgg cagtcatcct tagtctagaa cagcaagtca
     4801 gagagaggaa ccttaacccc aaagcagcct gccttaagag aagggaagaa gaaaaagttt
     4861 ctgccgtatc ggcagagccg ccaaccacac tgccaggaac ccatcctggg cttagtgaaa
     4921 ctaccaaccc tatgggtcat atgtaaatcg ataccgtcga cctcgagacc tagaaaaaca
     4981 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     5041 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     5101 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5161 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     5221 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     5281 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     5341 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     5401 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     5461 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5521 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5581 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5641 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     5701 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5761 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5821 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5881 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     5941 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6001 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6061 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6121 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6181 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6241 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6301 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6361 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6481 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6541 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6601 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6661 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6721 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     6781 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     6841 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     6901 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     6961 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     7021 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     7081 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     7141 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     7201 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7261 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     7321 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     7381 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7441 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7501 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7561 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7621 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7681 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8341 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9001 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9061 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9121 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9181 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9241 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9301 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9361 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9421 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9481 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9541 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9601 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9661 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     9721 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     9781 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     9841 agaatctgct tagggttagg cgttttgcgc tgcttcg
//
