LOCUS       sequence-416318-       10066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein quality control substrate and associated control YIp 
            ADE2-LEU2 pTDH3::ade1-G54V-GFP::tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Flagg MP, Lam B, Lam DK, Le TM, Kao A, Slaiwa YI, Hampton RY
  TITLE     Exploring the ""misfolding problem"" by systematic discovery and 
            analysis of functional-but-degraded proteins.
  JOURNAL   Mol Biol Cell. 2023 Dec 1;34(13):ar125. doi: 
            10.1091/mbc.E23-06-0248. Epub 2023 Sep 20.
  PUBMED    37729018
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2023-12-1"; volume: "34(13):ar125. doi"; pages: " 
            10.1091/mbc.E23-06-0248. Epub 2023 Sep 20"
COMMENT     Sequence Label: pRH3178
FEATURES             Location/Qualifiers
     source          1..10066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..669
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     562..585
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(701..717)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(702..718)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             1665..2375
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1692..1720)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2315..2335
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2399..2586
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2454..2478)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2590..2609)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2591..2609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2619..2636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2619..2635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2628..2650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2776..3231
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2863..2882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3073..3094
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3436..5151)
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     promoter        complement(5152..5527)
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     primer_bind     complement(5718..5737)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5837..5859
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5897..5915)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(6201..7295)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7296..7703)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        7871..7975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7976..8044,8045..8836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8194..8213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9007..9595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9496..9515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9749..9766
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9883..9904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9919..9924,9925..9942,9943..9949)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9957..9973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9962..9984
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9981..9997
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9981..9997
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10016..10036
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10018..10036
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccgctgct tggcggtcga gtttatcatt atcaatactg ccatttcaaa gaatacgtaa
       61 ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc ttttaattct
      121 gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat aacatcgtag
      181 gtgtctgggt gaacagttta ttcctggcat ccactaaata taatggagcc cgctttttaa
      241 gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt caccaaccat
      301 cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa acaggcaaaa
      361 aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg acacaaggca
      421 attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt ctgctctctc
      481 tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta ttcccctact
      541 tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat ctatttctta
      601 aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac accaagaact
      661 tagtttcgac ggatctgcag gaattcgata tcaagcttat cgataccgtc gacctcgaga
      721 tgtcaattac gaagactgaa ctggacggta tattgccatt ggtggccaga ggtaaagtta
      781 gagacatata tgaggtagac gctggtacgt tgctgtttgt tgctacggat cgtatctctg
      841 catatgacgt tattatggaa aacagcattc ctgaaaaggt gatcctattg accaaactgt
      901 cagagttctg gttcaagttc ctgtccaacg atgttcgtaa tcatttggtc gacatcgccc
      961 caggtaagac tattttcgat tatctacctg caaaattgag cgaaccaaag tacaaaacgc
     1021 aactagaaga ccgctctcta ttggttcaca aacataaact aattccattg gaagtaattg
     1081 tcagaggcta catcaccgga tctgcttgga aagagtacgt aaaaacaggt actgtgcatg
     1141 gtttgaaaca acctcaagga cttaaagaat ctcaagagtt cccagaacca atcttcaccc
     1201 catcgaccaa ggctgaacaa ggtgaacatg acgaaaacat ctctcctgcc caggccgctg
     1261 agctggtggg tgaagatttg tcacgtagag tggcagaact ggctgtaaaa ctgtactcca
     1321 agtgcaaaga ttatgctaag gagaagggca tcatcatcgc agacactaaa ttcgaattcg
     1381 gtattgacga aaagaccaat gaaattattc tagtggacga ggtgctaacg ccagactcct
     1441 ctagattctg gaacggtgcc tcttataagg taggagaatc ccaagattct tacgataagc
     1501 aatttttaag agactggctt actgctaata agttgaacgg tgttaacggc gtcaaaatgc
     1561 cccaagacat tgtcgacagg acaagggcca aatatataga ggcttatgaa acattgacag
     1621 ggtctaaatg gtctcacggt cgacggatcc ccgggttaat taacagtaaa ggagaagaac
     1681 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     1741 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     1801 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg
     1861 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
     1921 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     1981 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     2041 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     2101 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     2161 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     2221 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     2281 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     2341 ctgggattac acatggcatg gatgaactat acaaataggg cgcgccactt ctaaataagc
     2401 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
     2461 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
     2521 cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc
     2581 taccggcagc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     2641 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2701 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2761 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2821 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2881 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2941 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3001 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3061 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3121 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3181 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     3241 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagatctta tgtatgaaat
     3301 tcttaaaaaa ggacacctgt aagcgttgat ttctatgtat gaagtccaca tttgatgtaa
     3361 tcataacaaa gcctaaaaaa taggtatatc attttataat tatttgctgt acaagtatat
     3421 caataaactt atatattact tgttttctag ataagcttcg taaccgacag tttctaactt
     3481 ttgtgctttg acaagaactt cttcttcttg ctttaataaa aactgttcca ttttcgttgt
     3541 ataacttgaa tcataagcgc caagcagtct gacagccaac agcgcagcgt tcgtactatt
     3601 attaatagcg acggtagcta ctggaacacc tctaggcatt tgcacaattg aatgtaaaga
     3661 atctactcca tctagacaag aaccttttac gggcacaccg atgacaggaa gtggtgtcat
     3721 tgcagccacc atacctggca agtgagcagc cccaccagct ccagcgataa ttgttttaat
     3781 tccacgcttg cttgcggaaa tagcatatgc tgacatccta tgtggagttc tatgagcaga
     3841 gactattgtc acttcaaatg gaacgccaaa atcttttaaa accgcacatg cggcagacat
     3901 taccggcaag tcagagtctg atcccatgat gattccaacc aatggtttga ccattgcttc
     3961 caagtccaac ttttgagcga cagagatttt gattggaata tcagttctac ctgtaatgta
     4021 gttcagcctt tgttcacatt ccgccatact ggaggcaata atatttatgt gacctacttt
     4081 tctgttaggt ctagactctt ttccatataa gtacactgag gaacctggag tcgccaatgc
     4141 tctttcgcaa gtttctagct ctttatcttt tgtatgtttg tctccaagaa catttagcat
     4201 aatggcgttc gttgtaatgg tggagaaaga tgtgaaattc tttggcattg gcaaatccaa
     4261 tattgatctc aaatgagctt caaattgaga agtgacgcaa gcatcaatgg tataatgtcc
     4321 agagttgtga ggccttgggg caatttcgtt aataagcaat tcccctgttt ctaaatagaa
     4381 catttccaca ccaaatatac cacaaccggg aaaagatttg attgcatttt ctgccaacaa
     4441 cttcgcctta agttgaacgg agtccggaac tctagcaggc gcataacata agtcacaaat
     4501 attgtccttg tggatagtct ctacaattgg gtaagaaaac actaaaccgt taacagacct
     4561 cacaatcatg actgctaatt ctttagtaaa tggtgcccat ttttcggcgt acaaaggacg
     4621 atccttcagt acttccaaag cttccggaat catttcctta ttctttacaa cgaagttacc
     4681 tcttccatcg tatgccaaag tcctcgactt caagacgaat ggaaaaccca aatctcttcc
     4741 aacattcaat agggacgtct cactggcttg ttccacagga acactttggg taactgctat
     4801 accatttttg attaaatgct ctttttgaat atatttgtct tgtatcaatc tgattgtttc
     4861 tggagaaggg taaattttta atttgggatg ttttacttga agattcttta gtgtaggaac
     4921 atcaacatgc tcaatctcaa tcgttagcac atcacatttt tcagctagtt tttcgatatc
     4981 aagaggattg gaaaaggagc cattaacgtg gtcattggag ttgcttattt gtttggcagg
     5041 agaattttca gcatctagta ttaccgtctt aatgttgagc ctgtttgctg cctcaacaat
     5101 catacgtccc aattgtcccc ctcctaatat accaactgtt ctagaatcca tacttgattg
     5161 ttttgtccga ttttcttgtt tttcttgatt gttatagtag gatgtactta gaagagagat
     5221 ccaacgattt tacgcaccaa tttatacatg aaatgctcca taatattgtc catttagttc
     5281 ttaataaaag gtcagcaaga gtcaatcact tagtattacc cggttcgtag ccatgcaaca
     5341 agagtcattt gtcagcatag ctgtaataat caatcatgac gtaagaaatg tatcataatt
     5401 aaaagttgtt aaagatgtca gtgttatgtt ggtgttacaa aattctcggc ttctcactaa
     5461 tatttaatat ctcttaaatt ttatctgtct ttgattcttt taagaaaagt tatgtattat
     5521 tcaagaatta attcagatct attcttttga tttataaggg attttgccga tttcggccta
     5581 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5641 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     5701 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5761 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5821 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5881 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgatcttt
     5941 ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt tttccttatc
     6001 acgttgagcc attagtatca atttgcttac ctgtattcct ttacatcctc ctttttctcc
     6061 ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga acatattcca
     6121 ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat
     6181 cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca
     6241 ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgcatccaaa
     6301 acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa tgacaatttc
     6361 aacatcattg cagcagacaa gatagtggcg atagggttga ccttattctt tggcaaatct
     6421 ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag
     6481 gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc ttcatcggag
     6541 atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt tgggttctta
     6601 actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt agggaattcg
     6661 ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa aacattagct
     6721 ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa agcggccatt
     6781 cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc gacaccatca
     6841 ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct gacaacaacg
     6901 aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag agagtcggat
     6961 gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat ttttagtaaa
     7021 ccttgttcag gtctaacact accggtaccc catttaggac cacccacagc acctaacaaa
     7081 acggcatcag ccttcttgga ggcttccagc gcctcatctg gaagtggaac acctgtagca
     7141 tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt ggaacgaaca
     7201 tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc aacgtggtca
     7261 cctggcaaaa cgacgatctt cttaggggca gacattagaa tggtatatcc ttgaaatata
     7321 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa
     7381 ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat
     7441 gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc cggcgctctc
     7501 acctttcctt tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag gcgacctctg
     7561 aaattaacaa aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc ccctgtgtgt
     7621 tctcgttatg ttgaggaaaa aaataatggt tgctaagaga ttcgaactct tgcatcttac
     7681 gatacctgag tattcccaca gttaactgcg gtcaagatat ttcttgaatc aggcgcctta
     7741 gaccgctcgg ccaaacaacc aattacttgt tgagaaatag agtataatta tcctataaat
     7801 ataacgtttt tgaacacaca tgaacaagga agtacaggag acgtcaggtg gcacttttcg
     7861 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7921 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7981 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8041 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8101 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8161 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8221 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8281 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8341 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8401 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8461 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8521 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8581 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8641 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8701 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8761 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8821 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8881 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8941 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9001 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9061 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9661 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9721 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9781 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9841 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9901 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9961 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
    10021 taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggc
//
