LOCUS       sequence-416357-        4772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses luciferase under control of Mmp11 promoter in transfected 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Khader N, Shchuka VM, Dorogin A, Shynlova O, Mitchell JA
  TITLE     SOX4 exerts contrasting regulatory effects on labor-associated gene 
            promoters in myometrial cells.
  JOURNAL   PLoS One. 2024 Apr 18;19(4):e0297847. doi: 
            10.1371/journal.pone.0297847. eCollection 2024.
  PUBMED    38635533
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-04-18"; volume: "19(4):e0297847. doi"; pages: " 
            10.1371/journal.pone.0297847. eCollection 2024"
COMMENT     Sequence Label: pGL4.23_Mmp11
FEATURES             Location/Qualifiers
     source          1..4772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      363..372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             630..2282
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2326..2447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2337..2356)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2391..2410
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2695..2712)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2866..3454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2946..2965)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3654..4445,4446..4514))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4619..4667
                     /note="synthetic polyadenylation signal"
     misc_feature    4681..4772
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4721..4740
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc ttgtgccaag
       61 tgtcagtaga ggtcagaaga aggcatcagt tcctagatgg ccaccctgtg ggtgctagga
      121 acgggagcca ggtttctctg caggagcaac aagtgctcct aaccactgat ccatctttcc
      181 agacccgtct ctgttttgtt ttaaggtgtg gggactggcc aacttccgcc atatgcctca
      241 gtttcccctg aggtcacatt tcaatagtcc gctccttgca agagctattg taccactttc
      301 ctgttagcta gggctgttta gattggggat ctgaccacct gccacaggct aaaacaagtc
      361 aagccaccat ggagagacct ggcgaaggat gagacttctg aagtggggct ggagagcaga
      421 attgagctct ccccggctct cactagttct aagggaccca gcccctccgg gcactcctcc
      481 ctaactcaga ccgctgctgc aggccggttg agtttagaac aaaaggcagg gggaggcggg
      541 gcggtggggg gtgcggtccc ggcgccggcg ggggcggggc gaatgctata aggggcggcg
      601 gcccggcctg gcccagcagg cccagcacca tggaagatgc caaaaacatt aagaagggcc
      661 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
      721 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
      781 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
      841 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
      901 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
      961 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
     1021 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
     1081 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
     1141 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
     1201 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     1261 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     1321 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     1381 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     1441 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     1501 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     1561 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     1621 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     1681 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     1741 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     1801 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     1861 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     1921 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     1981 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     2041 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     2101 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     2161 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     2221 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     2281 aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     2341 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2401 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2461 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     2521 acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc
     2581 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     2641 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     2701 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2761 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2821 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2881 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2941 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3001 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3061 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3121 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3181 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3241 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3301 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3361 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3421 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3481 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3541 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3601 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
     3661 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
     3721 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
     3781 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
     3841 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
     3901 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
     3961 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
     4021 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
     4081 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
     4141 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
     4201 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
     4261 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
     4321 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
     4381 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
     4441 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
     4501 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
     4561 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
     4621 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     4681 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     4741 cagtgcaagt gcaggtgcca gaacatttct ct
//
