LOCUS       sequence-416358-        4953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses luciferase under control of Ptgs2 promoter in transfected 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Khader N, Shchuka VM, Dorogin A, Shynlova O, Mitchell JA
  TITLE     SOX4 exerts contrasting regulatory effects on labor-associated gene 
            promoters in myometrial cells.
  JOURNAL   PLoS One. 2024 Apr 18;19(4):e0297847. doi: 
            10.1371/journal.pone.0297847. eCollection 2024.
  PUBMED    38635533
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-04-18"; volume: "19(4):e0297847. doi"; pages: " 
            10.1371/journal.pone.0297847. eCollection 2024"
COMMENT     Sequence Label: pGL4.23_Ptgs2
FEATURES             Location/Qualifiers
     source          1..4953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             811..2463
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2507..2628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2518..2537)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2572..2591
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2876..2893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3047..3635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3127..3146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3835..4626,4627..4695))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4800..4848
                     /note="synthetic polyadenylation signal"
     misc_feature    4862..4953
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4902..4921
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tagcattccg
       61 atgaagtgga gctcagcatc tgtccctggg aaaggcgagt gccaggggct cctgtgactg
      121 cagaacccgg agggtagttc catgaaagac ttcaacctaa ttccaccagt acagatgtgg
      181 accctgacag aggacactca tttcattaaa aatagaagaa attaaattaa ccggtagctg
      241 tgtgcgtgct ctgagcagcg agcacgtcag actgcgcccc agtggggaga ggtgagggga
      301 ttcccttagt taggacctta gatcccggga ggggaagctg tgacactctt gagcttttag
      361 gcccccactg gatgcgcgac tgggaggaaa ccggagaccc caaagagcgc cagactaggc
      421 gcagactcag cgaaccacag ggcgcctgga gggatggaga gggcggtgca gctctcttgg
      481 caccacctgg ggcagccaag ggcagcttcc cggcttcctt cgtctctcat ttgcgtgggt
      541 aaaagcctgc cgctgcggtt cttgcgcaac tcactgaagc agagaggggg aaaagttggt
      601 gggggttggg gaaagcctaa gcggaaagac agagtcacca ctacgtcacg tggagtccgc
      661 tttacagact taaaagcaag gttctcccca ttagcggcca gttgtcaaac tgcgagctaa
      721 gagcttcagg agtcagtcag gactctgctc acgaaggaac tcagcactgc atcctgccag
      781 ctccaccgcc accactactg ccacctcccc atggaagatg ccaaaaacat taagaagggc
      841 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
      901 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
      961 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
     1021 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
     1081 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
     1141 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
     1201 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
     1261 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
     1321 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
     1381 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
     1441 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
     1501 atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc
     1561 accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag
     1621 gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca
     1681 ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac
     1741 gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc
     1801 ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg
     1861 atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag
     1921 gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg
     1981 tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct
     2041 ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag
     2101 cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc
     2161 ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc
     2221 ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt
     2281 aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag
     2341 aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg
     2401 gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg
     2461 taataattct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     2521 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2581 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2641 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2701 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
     2761 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     2821 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
     2881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3781 tatatgagta aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca
     3841 gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc
     3901 ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc
     3961 agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc
     4021 agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat
     4081 gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt
     4141 ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc
     4201 tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag
     4261 ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt
     4321 aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac
     4381 cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg
     4441 cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat
     4501 cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc
     4561 gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc
     4621 ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa
     4681 atgttggatg ctcatactcg tcctttttca atattattga agcatttatc agggttacta
     4741 gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca
     4801 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac
     4861 taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc
     4921 ccagtgcaag tgcaggtgcc agaacatttc tct
//
