LOCUS       sequence-416405-        9538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag GK
FEATURES             Location/Qualifiers
     source          1..9538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    805..1004
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1205..1621
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1551..1573
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1589..1605
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1639..1648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1645..1686
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1711..1734
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1741..1765
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3338..3362)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3421..3882
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3477..3494)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3704..3723
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3887..4681
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3941..3960)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4551..4570
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4868..5337
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5373..5477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5478..5546,5547..6338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5696..5715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8104..8692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8593..8612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8846..8863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8967..9394)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9077..9098)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9289..9308
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgcg
      601 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
      661 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
      721 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
      781 accaccgacc caccaccggg aggcaagctg gccagcaact tatctgtgtc tgtccgattg
      841 tctagtgtct atgactgatt ttatgcgcct gcgtcggtac tagttagcta actagctctg
      901 tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag
      961 acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg
     1021 gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc
     1081 gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca
     1141 gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1201 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1261 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1321 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1381 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1441 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1501 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1561 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1621 cggccggatc cagaattcgc caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
     1681 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
     1741 acaagtttgt acaaaaaagt tggcatggca gcctcaaaga aggcagtttt ggggccattg
     1801 gtgggggcgg tggaccaggg caccagttcg acgcgctttt tggttttcaa ttcaaaaaca
     1861 gctgaactac ttagtcatca tcaagtagaa ataaaacaag agttcccaag agaaggatgg
     1921 gtggaacagg accctaagga aattctacat tctgtctatg agtgtataga gaaaacatgt
     1981 gagaaacttg gacagctcaa tattgatatt tccaacataa aagctattgg tgtcagcaac
     2041 cagagggaaa ccactgtagt ctgggacaag ataactggag agcctctcta caatgctgtg
     2101 gtgtggcttg atctaagaac ccagtctacc gttgagagtc ttagtaaaag aattccagga
     2161 aataataact ttgtcaagtc caagacaggc cttccactta gcacttactt cagtgcagtg
     2221 aaacttcgtt ggctccttga caatgtgaga aaagttcaaa aggccgttga agaaaaacga
     2281 gctctttttg ggactattga ttcatggctt atttggagtt tgacaggagg agtcaatgga
     2341 ggtgtccact gtacagatgt aacaaatgca agtaggacta tgcttttcaa cattcattct
     2401 ttggaatggg ataaacaact ctgcgaattt tttggaattc caatggaaat tcttccaaat
     2461 gtccggagtt cttctgagat ctatggccta atgaaagctg gggccttgga aggtgtgcca
     2521 atatctgggt gtttagggga ccagtctgct gcattggtgg gacaaatgtg cttccagatt
     2581 ggacaagcca aaaatacgta tggaacagga tgtttcttac tatgtaatac aggccataag
     2641 tgtgtatttt ctgatcatgg ccttctcacc acagtggctt acaaacttgg cagagacaaa
     2701 ccagtatatt atgctttgga aggttctgta gctatagctg gtgctgttat tcgctggcta
     2761 agagacaatc ttggaattat aaagacctca gaagaaattg aaaaacttgc taaagaagta
     2821 ggtacttctt atggctgcta cttcgtccca gcattttcgg ggttatatgc accttattgg
     2881 gagcccagcg caagagggat aatctgtgga ctcactcagt tcaccaataa atgccatatt
     2941 gcttttgctg cattagaagc tgtttgtttc caaactcgag agattttgga tgccatgaat
     3001 cgagactgtg gaattccact cagtcatttg caggtagatg gaggaatgac cagcaacaaa
     3061 attcttatgc agctacaagc agacattctg tatataccag tagtgaagcc ctcaatgccc
     3121 gaaaccactg cactgggtgc ggctatggcg gcaggggctg cagaaggagt cggcgtatgg
     3181 agtctcgaac ccgaggattt gtctgccgtc acgatggagc ggtttgaacc tcagattaat
     3241 gcggaggaaa gtgaaattcg ttattctaca tggaagaaag ctgtgatgaa gtcaatgggt
     3301 tgggttacaa ctcaatctcc agaaagtggt attccattgc caactttctt gtacaaagtg
     3361 gtttgatcta gtcgaccaat tccggttatt ttccaccata ttgccgtctt ttggcaatgt
     3421 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     3481 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     3541 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     3601 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     3661 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     3721 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     3781 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     3841 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccatga ttgaacaaga
     3901 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     3961 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4021 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     4081 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4141 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4201 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4261 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4321 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4381 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     4441 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4501 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4561 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4621 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4681 agcgggactc tggggttcgc atcgataaaa taaaagattt tatttagtct ccagaaaaag
     4741 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     4801 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
     4861 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     4921 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
     4981 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
     5041 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     5101 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     5161 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
     5221 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     5281 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacag
     5341 gccagtgggg ccgcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5401 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5461 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5521 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5581 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5641 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5701 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5761 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5821 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5881 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5941 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6001 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6061 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6121 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6181 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6241 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6301 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6361 atatacttta gattgatttg cggccgcgaa aaggacaagc agcgaaaatt cacgccccct
     6421 tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc
     6481 tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac
     6541 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc
     6601 tacccagata taaattagga tagcatatac tacccagata tagattagga tagcatatgc
     6661 tacccagata tagattagga tagcctatgc tacccagata tagattagga tagcatatgc
     6721 tatccagata tttgggtagt atatgctacc catggcaaca ttagcccacc gtgctctcag
     6781 cgacctcgtg aatatgagga ccaacaaccc tgtgcttggc gctcaggcgc aagtgtgtgt
     6841 aatttgtcct ccagatcgca gcaatcgcgc ccctatcttg gcccgcccac ctacttatgc
     6901 aggtattccc cggggtgcca ttagtggttt tgtgggcaag tggtttgacc gcagtggtta
     6961 gcggggttac aatcagccaa gttattacac ccttatttta cagtccaaaa ccgcagggcg
     7021 gcgtgtgggg gctgacgcgt gcccccactc cacaatttca aaaaaaagag tggccacttg
     7081 tctttgttta tgggccccat tggcgtggag ccccgtttaa ttttcggggg tgttagagac
     7141 aaccagtgga gtccgctgct gtcggcgtcc actctctttc cccttgttac aaatagagtg
     7201 taacaacatg gttcacctgt cttggtccct gcctgggaca catcttaata accccagtat
     7261 catattgcac taggattatg tgttgcccat agccataaat tcgtgtgaga tggacatcca
     7321 gtctttacgg cttgtcccca ccccatggat ttctattgtt aaagatattc agaatgtttc
     7381 attcctacac tagtatttat tgcccaaggg gtttgtgagg gttatattgg tgtcatagca
     7441 caatgccacc actgaacccc ccgtccaaat tttattctgg gggcgtcacc tgaaaccttg
     7501 ttttcgagca cctcacatac accttactgt tcacaactca gcagttattc tattagctaa
     7561 acgaaggaga atgaagaagc aggcgaagat tcaggagagt tcactgcccg ctccttgatc
     7621 ttcagccact gcccttgtga ctaaaatggt tcactaccct cgtggaatcc tgaccccatg
     7681 taaataaaac cgtgacagct catggggtgg gagatatcgc tgttccttag gaccctttta
     7741 ctaaccctaa ttcgatagca tatgcttccc gttgggtaac atatgctatt gaattagggt
     7801 tagtctggat agtatatact actacccggg aagcatatgc tacccgttta gggttaacaa
     7861 gggggcctta taaacactat tgctaatgcc ctcttgaggg tccgcttatc ggtagctaca
     7921 caggcccctc tgattgacgt tggtgtagcc tcccgtagtc ttcctgggcc cctgggaggt
     7981 acatgtcggc cgcaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8041 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8101 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8161 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8221 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8281 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8341 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8401 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8461 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8521 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8581 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8641 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8701 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8761 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8821 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8881 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcaact atggttggcg
     8941 cgttgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     9001 ttttaaccaa tagaccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat
     9061 agagttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     9121 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa
     9181 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     9241 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     9301 gaaaggagcg ggcgctaagg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     9361 acccgccgcg cttaatgcgc cgctacaggg cgcgccgatc ttcaatattg gccattagcc
     9421 atattattca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
     9481 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgtt
//
