LOCUS       sequence-416406-        4734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A17_STOP
FEATURES             Location/Qualifiers
     source          1..4734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2859..2958)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2975..2994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2976..2994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2999..3015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2999..3015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3117..3136)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3128..3937
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3195..3214)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4084..4672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4573..4592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgccca agaacagcaa ggtgacccag cgggagcaca gctccgagca
      721 cgtgacagag tccgtggcag acctgctggc cctggaggag cctgtggatt acaagcagtc
      781 cgtgctgaac gtggcaggag aggcaggagg caagcagaag gcagtggagg aggagctgga
      841 cgcagaggat aggcctgcct ggaattctaa gctgcagtac atcctggcac agatcggctt
      901 cagcgtggga ctgggcaaca tctggcgctt tccatatctg tgccagaaga atggcggcgg
      961 cgcctacctg gtgccttatc tggtgctgct gatcatcatc ggcatcccac tgttctttct
     1021 ggagctggca gtgggacaga ggatcaggag aggaagcatc ggcgtgtggc actacatctg
     1081 tccacgcctg ggcggcatcg gcttctctag ctgcatcgtg tgcctgtttg tgggcctgta
     1141 ctataacgtg atcatcggct ggtctatctt ctacttcttt aagagctttc agtatcctct
     1201 gccatggagc gagtgcccag tggtgcggaa tggatccgtg gcagtggtgg aggcagagtg
     1261 tgagaagtcc tctgccacca catacttctg gtatagagag gccctggaca tcagcgattc
     1321 catctctgag agcggcggcc tgaactggaa gatgaccctg tgcctgctgg tggcctggag
     1381 catcgtgggc atggccgtgg tgaagggcat ccagagctcc ggcaaagtga tgtacttctc
     1441 tagcctgttt ccctatgtgg tgctggcctg tttcctggtg cggggactgc tgctgagagg
     1501 agccgtggac ggcatcctgc acatgtttac ccccaagctg gacaagatgc tggatcctca
     1561 agtgtggagg gaggccgcca cacaggtgtt ctttgcactg ggactgggat tcggaggcgt
     1621 gatcgccttt tcctcttaca acaagcagga caacaattgc cacttcgatg ccgccctggt
     1681 gtcctttatc aatttcttta cctctgtgct ggccacactg gtggtgttcg ccgtgctggg
     1741 ctttaaggcc aacatcatga atgagaagtg cgtggtggag aacgccgaga agatcctggg
     1801 ctatctgaac accaatgtgc tgtcccgcga cctgatcccc cctcacgtga acttctctca
     1861 cctgaccaca aaggattaca tggagatgta taatgtgatc atgaccgtga aggaggacca
     1921 gttttctgcc ctgggactgg atccatgcct gctggaggac gagctggata agagcgtgca
     1981 gggcacaggc ctggccttca tcgcctttac cgaggccatg acacacttcc cagcctcccc
     2041 cttttggtct gtgatgttct ttctgatgct gatcaatctg ggcctgggct ccatgatcgg
     2101 caccatggcc ggcatcacca caccaatcat cgacaccttc aaggtgccca aggagatgtt
     2161 tacagtgggc tgctgcgtgt tcgcctttct ggtgggactg ctgttcgtgc agcggtccgg
     2221 caactacttc gtgacaatgt ttgacgatta ttctgccacc ctgcctctga cactgatcgt
     2281 gatcctggag aatatcgccg tggcctggat ctacggcacc aagaagttca tgcaggagct
     2341 gacagagatg ctgggcttta ggccctatcg cttctacttt tatatgtgga agttcgtgag
     2401 ccctctgtgc atggccgtgc tgaccacagc ctccatcatc cagctgggag tgaccccacc
     2461 aggatacagc gcctggatca aggaggaggc agcagagcgg tacctgtatt ttcctaactg
     2521 ggccatggcc ctgctgatca ccctgatcgt ggtggccaca ctgcccatcc ctgtggtgtt
     2581 cgtgctgaga cactttcacc tgctgtctga tggcagcaat acactgtccg tgtcttacaa
     2641 gaagggccgg atgatgaagg acatctccaa cctggaggag aatgatgaga ccagattcat
     2701 cctgagcaag gtgccatccg aggccccatc tcccatgcct acacacagga gctatctggg
     2761 acctggaagc acctccccac tggagacatc tggcaaccca aatggccgct acggaagcgg
     2821 atatctgctg gcaagcaccc ccgagtccga gctgtaagac ccagctttct tgtacaaagt
     2881 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2941 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     3001 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     3061 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     3121 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     3181 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     3241 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     3301 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     3361 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     3421 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     3481 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     3541 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3601 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     3661 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     3721 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3781 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3841 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3901 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3961 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     4021 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
