LOCUS       sequence-416439-        1589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Designed for cytoplasmic E.coli overexpression of glucanase 
            (beta-1,3-glycosidase) for gentle yeast spheroblasting that unlike 
            Zymolyase prevents proteolysis of intracellular components. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Kuznetsov VI, Haws SA, Fox CA, Denu JM
  TITLE     General method for rapid purification of native chromatin fragments.
  JOURNAL   J Biol Chem. 2018 Aug 3;293(31):12271-12282. doi: 
            10.1074/jbc.RA118.002984. Epub 2018 May 24.
  PUBMED    29794135
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.002984"; journalName: "J Biol Chem"; date: 
            "2018-08-3- 3"; volume: "293"; issue: "31"; pages: "12271-12282"
COMMENT     Sequence Label: pT22-GluCNZ-6His
FEATURES             Location/Qualifiers
     source          1..1589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1569..1586
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aactttaaga aggagatata catatggtcc ccgcgaccat cccgctgacg atcaccaacg
       61 actcgggcag ggggccgatc tacctgtacg tcctcggcga gcgcgacggc gtcgccggct
      121 gggcggacgc gggcggcacg ttccacccgt ggcccggcgg ggtcgggccc gtgcccgtcc
      181 cggcacccga cgcgtccatc gcgggccccg gccccggcca gtccgtgacg atccgactcc
      241 cgaagctgtc cgggcgcgtc tactactcgt acggccagaa gatgacgttc cagatcgtgc
      301 tcgacgggcg gctcgtccag cccgccgtcc agaacgactc cgaccccaac cggaacatcc
      361 tcttcaactg gaccgagtac acgctcaacg acggcggcct gtggatcaac agcacgcagg
      421 tggaccactg gtccgcgccg taccaggtgg gcgtccagcg cgccgacggg caggtcctca
      481 gcacgggcat gctcaagccg aacggctacg aggcgttcta cacggccctc gagggcgcgg
      541 ggtggggcgg gctcgtgcag cgcgcgcccg acgggagccg cctgcgcgcg ctcaacccgt
      601 cgcacgggat cgacgtcggg aagatctcgt cggcctcgat cgactcctac gtcaccgagg
      661 tgtggaactc gtaccgcacg cgcgacatgg tcgtcacgcc gttctcccac gagcccggca
      721 cgcagttccg cggccgggtc gacggcgact ggttccgctt caggagcggg tccgggcagg
      781 aggtcgccgc gttcaagaag cccgacgcgt cgagcgtgta cgggtgccac aaggacctcc
      841 aggcgcccaa cgaccacgtc gtcgggccga tcgcccgcac cctgtgcgcc gcgctcgtcc
      901 gcaccacggc gctgaccaac ccgaaccagc cggacgcgaa cagcgccggc ttctaccagg
      961 acgcgcgcac caacgtctac gcgaagctcg cgcaccagca gatggcgaac ggcaaggcgt
     1021 acgcgttcgc gttcgacgac gtcggcgcgc acgagtcgct cgtccacgac ggcaaccccc
     1081 aggccgcgta catcaagctc gacccgttca ccggcacggc cacgcccctc gggaacggcg
     1141 gcagcaccga gcagccgggc acccccggcg gtctgcccgc cgggacgggc gcgctccgca
     1201 tcgggagcac gctgtgcctc gacgtcccgt gggccgaccc gaccgacacc aaccaggtcc
     1261 agctcgccac ctgcagcggc aacgcggcgc agcagtggac gcgcggcacc gacgggacgg
     1321 tgcgcgccct cggcaagtgt ctcgacgtcg cgcgcagcgg gacggccgac ggcaccgccg
     1381 tgtggatcta cacgtgcaac gggacgggcg cgcagaagtg gacgtacgac tccgcgacca
     1441 aggccctgcg caacccgcag tccggcaagt gcctcgacgc ccagggcggc gcaccgctgc
     1501 gtgacggcca gaaggtccag ctctggacct gcaaccagac cgaggcccag cgctggacgc
     1561 tcctcgagca ccaccaccac caccactga
//
