LOCUS       sequence-416474-       10704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEST vector to tag gene of interest with DHFR F[1,2] on the 
            N-terminus to perform DHFR-PCA in S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, 
            Gagnon-Arsenault I, Landry CR
  TITLE     Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs 
            following a whole-genome duplication event.
  JOURNAL   Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
  PUBMED    37979156
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246."
COMMENT     Sequence Label: pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
FEATURES             Location/Qualifiers
     source          1..10704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(30..48)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(69..85)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(69..85)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(82..104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    93..109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(117..123,124..141,142..147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    162..183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(300..317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(471..1059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(551..570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1230..2021,2022..2090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1853..1872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2091..2195)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2232..2735
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2776..2794
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2832..2854)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2954..2973
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      complement(3125..3312)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(3320..3892)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(3893..4236)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     rep_origin      complement(4375..4830)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4512..4533)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4724..4743
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4956..4978
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4970..4987
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4971..4987
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4994..5013
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4994..5012
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(5165..5352)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     protein_bind    5611..5735
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5776..6081)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5878..5897)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(6401..7081)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     6996..7015
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7082..7184)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(7331..7455)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(7808..8512)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      complement(9322..9569)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     9535..9553
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 atagaatctg gggatcccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
       61 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      121 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      181 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      901 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2161 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2221 gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta atataaatat
     2281 ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga
     2341 agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg
     2401 ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa
     2461 tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt
     2521 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
     2581 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
     2641 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
     2701 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat tattatcatg
     2761 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     2821 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     2881 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     2941 tggcttaact atgcggcatc agagcagatt gtactgagag atcgatgaat tcgagctcga
     3001 ttacaacagg tgttgtcctc tgaggacata aaatacacac cgagattcat caactcattg
     3061 ctggagttag catatctaca attgggtgaa atggggagcg atttgcaggc atttgctcgg
     3121 catgccggta gaggtgtggt caataagagc gacctcatgc tatacctgag aaagcaacct
     3181 gacctacagg aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact
     3241 ttaaaatttg tatacactta ttttttttat aacttattta ataataaaaa tcataaatca
     3301 taagaaattc gcctcgagat taggggcagg gcatgctcat gtagagcgcc tgctcgccgt
     3361 ccgaggcggt gccgtcgtac agggcggtgt ccaggccgca gagggtgaac cccatccgcc
     3421 ggtacgcgtg gatcgccggt gcgttgacgt tggtgacctc cagccagagg tgcccggcgc
     3481 cccgctcgcg ggcgaactcc gtcgcgagcc ccatcaacgc gcgcccgacc ccgtgccccc
     3541 ggtgctccgg ggcgacctcg atgtcctcga cggtcagccg gcggttccag ccggagtacg
     3601 agacgaccac gaagcccgcc aggtcgccgt cgtccccgta cgcgacgaac gtccgggagt
     3661 ccgggtcgcc gtcctccccg tcgtccgatt cgtcgtccga ttcgtcgtcg gggaacacct
     3721 tggtcagggg cgggtccacc ggcacctccc gcagggtgaa gccgtccccg gtggcggtga
     3781 cgcggaagac ggtgtcggtg gtgaaggacc catccagtgc ctcgatggcc tcggcgtccc
     3841 ccgggacact ggtgcggtac cggtaagccg tgtcgtcaag agtggtaccc atggttgttt
     3901 atgttcggat gtgatgtgag aactgtatcc tagcaagatt ttaaaaggaa gtatatgaaa
     3961 gaagaacctc agtggcaaat cctaaccttt tatatttctc tacaggggcg cggcgtgggg
     4021 acaattcaac gcgtctgtga ggggagcgtt tccctgctcg caggtctgca gcgaggagcc
     4081 gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg gatgcaaatg attatacatg
     4141 gggatgtatg ggctaaatgt acgggcgaca gtcacatcat gcccctgagc tgcgcacgtc
     4201 aagactgtca aggagggtat tctgggcctc catgtcgctg gccgggtgac ccggcgggga
     4261 cgaggcaagc taaacagatc tggcgcgcct taattaaccc ggggatccgt cgacctgcag
     4321 cgtacgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     4381 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     4441 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     4501 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     4561 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     4621 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4681 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4741 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4801 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     4861 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     4921 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     4981 ggccagtgaa ttgtaatacg actcactata gggcgaattg ggatccgtgt ggaagaacga
     5041 ttacaacagg tgttgtcctc tgaggacata aaatacacac cgagattcat caactcattg
     5101 ctggagttag catatctaca attgggtgaa atggggagcg atttgcaggc atttgctcgg
     5161 catgccggta gaggtgtggt caataagagc gacctcatgc tatacctgag aaagcaacct
     5221 gacctacagg aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact
     5281 ttaaaatttg tatacactta ttttttttat aacttattta ataataaaaa tcataaatca
     5341 taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacgattt gacccttttc
     5401 catcttttcg taaatttctg gcaaggtaga caagccgaca accttgattg gagacttgac
     5461 caaacctctg gcgaagaagt ccaaagctcc accgcggtgg cggccgttac ttacttagag
     5521 cttattaccc tgttatccct aacccataat acccataata gctgtttgcc attacttact
     5581 taaagctctt acttacttag cggccatcaa accactttgt acaagaaagc tgaacgagaa
     5641 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     5701 taatactgta aaacacaaca tatccagtca ctatggtcga cctgcagact ggctgtgtat
     5761 aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
     5821 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat
     5881 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
     5941 ggggacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg
     6001 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
     6061 ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa gagccgttca
     6121 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg
     6181 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat
     6241 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
     6301 catagcatac ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg
     6361 caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc ggatcctcta
     6421 gattacgccc cgcctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     6481 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     6541 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     6601 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     6661 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     6721 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     6781 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     6841 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     6901 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     6961 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     7021 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     7081 ttttagcttc cttagctcct gaaaatctcg atgactcaaa aaatacgccc ggtagtgatc
     7141 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     7201 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     7261 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtagggtgat gctgccaact
     7321 tagcggccgc catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     7381 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     7441 tttgtacaaa cttgtactag tacttcctcc tccggtaccc aattccggtt gttcaataag
     7501 tcttaaggca tcatccaaac ttttggcaag aaaatgagct cctcgtggtg gttctttgag
     7561 ttctctactg agaactatat taattctgtc ctttaaaggt cgattcttct caggaatgga
     7621 gaaccaggtt ttcctaccca taatcaccag attctgttta ccttccactg aagaggttgt
     7681 ggtcattctt tggaagtact tggactcgtt cctgagcgga ggccagggat agtctccgtt
     7741 cttgccaatc cccatatttt gggacacggc gacgatgcag ttcaatggtc gaaccatcca
     7801 agcttggagt tgattgtatg cttggtatag cttgaaatat tgtgcagaaa aagaaacaag
     7861 gaagaaaggg aacgagaaca atgacgagga aacaaaagat taataattgc aggtctattt
     7921 atacttgata gcaagacagc aaactttttt ttatttcaaa ttcaagtaac tggaaggaag
     7981 gccgtatacc gttgctcatt agagagtagt gtgcgtgaat gaaggaagga aaaagtttcg
     8041 tgtgcttcga gatacccctc atcagctctg gaacaacgac atctgttggt gctgtctttg
     8101 tcgttaattt tttcctttag tgtcttccat catttttttg tcattgcgga tatggtgaga
     8161 caacaacggg ggagagagaa aagaaaaaaa aagaaaagaa gttgcatgcg cctattatta
     8221 cttcaataga tggcaaatgg aaaaagggta gtgaaacttc gatatgatga tggctatcaa
     8281 gtctagggct acagtattag ttcgttatgt accaccatca atgaggcagt gtaattggtg
     8341 tagtcttgtt tagcccatta tgtcttgtct ggtatctgtt ctattgtata tctcccctcc
     8401 gccacctaca tgttagggag accaacgaag gtattatagg aatcccgatg tatgggtttg
     8461 gttgccagaa aagaggaagt ccatattgta cacccggaaa caacaaaagg atatccgaaa
     8521 tattccacgg tttagaaaaa aatcggaaaa gagcgcggag gggtgttacc ccccttctct
     8581 actagcattg gactttaatt tcgctacctt aggaccgtta tagttacatt aatatatgtg
     8641 cataggagaa gtgtaaagtt cccttccata ttgtaacata ataaagtgca cacccaaatg
     8701 aattgaaagc gtactcaaac agacaaccat ttccagtgtt gtatgtacct gtctatttat
     8761 actggtagca accctattgc tgtttcctct tcaaagtact ctagcggtta tgcgcgtctc
     8821 accttcaagg tcatggtcgc tctattgttc gcaccaccgg caaactcgcg tctcgcaagt
     8881 cttggctcat tcttctagta tactcatgtt gcaaatgcac tcaggttctt tcggcaactt
     8941 aaataatgac accagttgtc gtggtcgtca tcatcgcaac cccaaccggc attcttattg
     9001 cttctccaat ctcgcccctt agcgcagggt aaaccttgga aaatgcaggc gcaaaaaact
     9061 ccgccgggca cagcctcacg cccagcgtta tcgccgggcc ggcaagagcg cgggtccgcc
     9121 acagagtcag catgattgtg caattgcgta aactcgtttt ttcggcgccg caaagccaaa
     9181 tacatcatat caacactttt cactttattt ttcgttcgac ccttatattt gtcttttgcc
     9241 ttcatgctcc ttgatttcct atttcattta ccatcatttc ttcgatcccg gatctcgagg
     9301 gggggcccgg atcccgagct tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag
     9361 caaggttttc agtataatgt tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat
     9421 ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta
     9481 gacttcaggt tgtctaactc cttccttttc ggttagagcg gatgtggggg gagggcgtga
     9541 atgtaagcgt gacataacta attacatgat atcgacaaag gaaaaggggc ctgtttactc
     9601 acaggctttt ttcaagtagg taattaagtc gtttctgtct ttttccttct tcaacccacc
     9661 aaaggccatc ttggtacttt tttttttttt tttttttttt tttttttttt tttttttttt
     9721 tgatagaaat aatacagaag tagatgttga attagattaa actgaagata tataatttat
     9781 tggaaaatac atagagtttt tgttgatgcg cttaagcgat caattcaaca acaccaccag
     9841 cagctctgat tttttcttca gccaacttgg agacgaatct agctttgacg ataactggaa
     9901 catttggaat tctaccctta cccaagatct taccgtaacc ggctgccaaa gtgtcaataa
     9961 ctggagcagt ttccttagaa gcagatttca agtattggtc tctcttgtct tctgggatca
    10021 atgtccacaa tttgtccaag ttcaagactg gcttccagaa atgagcttgt tgcttgtgga
    10081 agtatctcat accaacctta ccgaaataac ctggatggta tttatccatg ttaattctgt
    10141 ggtgatgttg accaccggcc atacctctac caccggggtg ctttctgtgc ttaccgatac
    10201 gacctttacc ggctgagacg tgacctctgt gctttctagt cttagtgaat ctggaaggca
    10261 ttcttgatta gttggatgat tgttctggga tttaatgcaa aaaaatcact aagaaggaaa
    10321 aaaatcaacg gagaaagcaa acgccatctt aaatatacgg gatacagatg aaaggtttga
    10381 acctatctgg gaaaatacgc attaaacaag cgaaaaactg cgaggaaaat tgtttgcgtc
    10441 tctgcgggct attcacgcgc cagaggaaaa ataggaaaaa taacaggcat tagaaaaata
    10501 attttgattt tggtaatgtg tgggtccctg gtgtacagat gttacattgg ttacagtact
    10561 cttgtttttg ctgtgttttt cgatgaatct ccaaaatggt tgttagcaca tggaagagtc
    10621 accgatgcta agttatctct atgtaagcta cgtggcgtga cttttgatgg agccgcacaa
    10681 gagatacagg attggcaact gcaa
//
