LOCUS       sequence-416475-       10397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEST vector with NatR marker for gene expression in S. cerevisiae. 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, 
            Gagnon-Arsenault I, Landry CR
  TITLE     Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs 
            following a whole-genome duplication event.
  JOURNAL   Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
  PUBMED    37979156
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246."
COMMENT     Sequence Label: pDN0603 (pDEST, NatR)
FEATURES             Location/Qualifiers
     source          1..10397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    709..833
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1007..1109
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1110..1790
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1176..1195)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2110..2415
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2294..2313
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2456..2580)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2845..3032
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3184..3203)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3185..3203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3210..3227)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3210..3226)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3219..3241)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3367..3822
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3454..3473)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3664..3685
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3961..4304
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4305..4877
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      4885..5072
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5224..5243)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5343..5365
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5403..5421)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5462..5965
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6002..6106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6107..6175,6176..6967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6325..6344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7138..7726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7627..7646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7880..7897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8014..8035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8050..8055,8056..8073,8074..8080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8088..8104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8093..8115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8112..8128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8112..8128
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8149..8167
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      9333..9580
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(9349..9367)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gttgtttccg ggtgtacaat atggacttcc tcttttctgg caaccaaacc catacatcgg
       61 gattcctata ataccttcgt tggtctccct aacatgtagg tggcggaggg gagatataca
      121 atagaacaga taccagacaa gacataatgg gctaaacaag actacaccaa ttacactgcc
      181 tcattgatgg tggtacataa cgaactaata ctgtagccct agacttgata gccatcatca
      241 tatcgaagtt tcactaccct ttttccattt gccatctatt gaagtaataa taggcgcatg
      301 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca
      361 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac
      421 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc
      481 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact
      541 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt
      601 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg
      661 cacaatattt caagctatac caagcataca aagggtgggt cgaatcaaac aagtttgtac
      721 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      781 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
      841 cgggtgatgc tgccaactta gcggccgcta agttggcagc atcacccgac gcactttgcg
      901 ccgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg
      961 ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa
     1021 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagtcat
     1081 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     1141 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     1201 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     1261 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     1321 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     1381 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     1441 acgatttccg gcagtttcta catatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     1501 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     1561 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1621 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     1681 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     1741 actgcgatga gtggcaggcg gggcgtaatc tagaggatcc ggcttactaa aagccagata
     1801 acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac
     1861 ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc
     1921 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa
     1981 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag
     2041 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg
     2101 gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
     2161 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc
     2221 agtgcacgtc tgctgtcaga taaagtcccc cgtgaacttt acccggtggt gcatatcggg
     2281 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
     2341 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
     2401 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag
     2461 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
     2521 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
     2581 aggaggagga agtactagtt taattaagag ctcttaagta agtaatggca aacagctatt
     2641 atgggtatta tgggttaggg ataacagggt aataagctct aagtaagtaa cggccgccac
     2701 cgcggtggag ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc
     2761 ggcttgtcta ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga
     2821 tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
     2881 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     2941 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat
     3001 gaggtcgctc ttattgacca cacctctacc ggcatgccga gcaaatgcct gcaaatcgct
     3061 ccccatttca cccaattgta gatatgctaa ctccagcaat gagttgatga atctcggtgt
     3121 gtattttatg tcctcagagg acaacacctg ttgtaatcgt tcttccacac ggatcccaat
     3181 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     3241 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3301 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3361 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3421 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3481 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3541 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3601 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3661 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3721 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3781 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     3841 ccttacgcat ctgtgcggta tttcacaccg cgtacgctgc aggtcgacgg atccccgggt
     3901 taattaaggc gcgccagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc
     3961 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
     4021 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
     4081 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
     4141 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
     4201 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
     4261 ttgctaggat acagttctca catcacatcc gaacataaac aaccatgggt accactcttg
     4321 acgacacggc ttaccggtac cgcaccagtg tcccggggga cgccgaggcc atcgaggcac
     4381 tggatgggtc cttcaccacc gacaccgtct tccgcgtcac cgccaccggg gacggcttca
     4441 ccctgcggga ggtgccggtg gacccgcccc tgaccaaggt gttccccgac gacgaatcgg
     4501 acgacgaatc ggacgacggg gaggacggcg acccggactc ccggacgttc gtcgcgtacg
     4561 gggacgacgg cgacctggcg ggcttcgtgg tcgtctcgta ctccggctgg aaccgccggc
     4621 tgaccgtcga ggacatcgag gtcgccccgg agcaccgggg gcacggggtc gggcgcgcgt
     4681 tgatggggct cgcgacggag ttcgcccgcg agcggggcgc cgggcacctc tggctggagg
     4741 tcaccaacgt caacgcaccg gcgatccacg cgtaccggcg gatggggttc accctctgcg
     4801 gcctggacac cgccctgtac gacggcaccg cctcggacgg cgagcaggcg ctctacatga
     4861 gcatgccctg cccctaatct cgaggcgaat ttcttatgat ttatgatttt tattattaaa
     4921 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     4981 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat
     5041 gaggtcgctc ttattgacca cacctctacc ggcatgccga gcaaatgcct gcaaatcgct
     5101 ccccatttca cccaattgta gatatgctaa ctccagcaat gagttgatga atctcggtgt
     5161 gtattttatg tcctcagagg acaacacctg ttgtaatcga gctcgaattc atcgatctct
     5221 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5281 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5341 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5401 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5461 ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa taatttggga
     5521 atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa gtaaataaag
     5581 aaggtagaag agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac
     5641 aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca
     5701 ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca
     5761 gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag
     5821 tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc
     5881 tttaatttct ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat
     5941 tataattatt tttatagcac gtgatgaaaa ggacccaggt ggcacttttc ggggaaatgt
     6001 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7801 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7861 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7921 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7981 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8041 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8101 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgaaa ttaaccctca
     8161 ctaaagggaa caaaagctgg gatccccaga ttctatttgc agttgccaat cctgtatctc
     8221 ttgtgcggct ccatcaaaag tcacgccacg tagcttacat agagataact tagcatcggt
     8281 gactcttcca tgtgctaaca accattttgg agattcatcg aaaaacacag caaaaacaag
     8341 agtactgtaa ccaatgtaac atctgtacac cagggaccca cacattacca aaatcaaaat
     8401 tatttttcta atgcctgtta tttttcctat ttttcctctg gcgcgtgaat agcccgcaga
     8461 gacgcaaaca attttcctcg cagtttttcg cttgtttaat gcgtattttc ccagataggt
     8521 tcaaaccttt catctgtatc ccgtatattt aagatggcgt ttgctttctc cgttgatttt
     8581 tttccttctt agtgattttt ttgcattaaa tcccagaaca atcatccaac taatcaagaa
     8641 tgccttccag attcactaag actagaaagc acagaggtca cgtctcagcc ggtaaaggtc
     8701 gtatcggtaa gcacagaaag caccccggtg gtagaggtat ggccggtggt caacatcacc
     8761 acagaattaa catggataaa taccatccag gttatttcgg taaggttggt atgagatact
     8821 tccacaagca acaagctcat ttctggaagc cagtcttgaa cttggacaaa ttgtggacat
     8881 tgatcccaga agacaagaga gaccaatact tgaaatctgc ttctaaggaa actgctccag
     8941 ttattgacac tttggcagcc ggttacggta agatcttggg taagggtaga attccaaatg
     9001 ttccagttat cgtcaaagct agattcgtct ccaagttggc tgaagaaaaa atcagagctg
     9061 ctggtggtgt tgttgaattg atcgcttaag cgcatcaaca aaaactctat gtattttcca
     9121 ataaattata tatcttcagt ttaatctaat tcaacatcta cttctgtatt atttctatca
     9181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagtacca agatggcctt
     9241 tggtgggttg aagaaggaaa aagacagaaa cgacttaatt acctacttga aaaaagcctg
     9301 tgagtaaaca ggcccctttt cctttgtcga tatcatgtaa ttagttatgt cacgcttaca
     9361 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     9421 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     9481 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
     9541 gcttgagaag gttttgggac gctcgaaggc tttaatttgc aagctcggga tccgggcccc
     9601 ccctcgagat ccgggatcga agaaatgatg gtaaatgaaa taggaaatca aggagcatga
     9661 aggcaaaaga caaatataag ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt
     9721 atttggcttt gcggcgccga aaaaacgagt ttacgcaatt gcacaatcat gctgactctg
     9781 tggcggaccc gcgctcttgc cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg
     9841 gagttttttg cgcctgcatt ttccaaggtt taccctgcgc taaggggcga gattggagaa
     9901 gcaataagaa tgccggttgg ggttgcgatg atgacgacca cgacaactgg tgtcattatt
     9961 taagttgccg aaagaacctg agtgcatttg caacatgagt atactagaag aatgagccaa
    10021 gacttgcgag acgcgagttt gccggtggtg cgaacaatag agcgaccatg accttgaagg
    10081 tgagacgcgc ataaccgcta gagtactttg aagaggaaac agcaataggg ttgctaccag
    10141 tataaataga caggtacata caacactgga aatggttgtc tgtttgagta cgctttcaat
    10201 tcatttgggt gtgcacttta ttatgttaca atatggaagg gaactttaca cttctcctat
    10261 gcacatatat taatgtaact ataacggtcc taaggtagcg aaattaaagt ccaatgctag
    10321 tagagaaggg gggtaacacc cctccgcgct cttttccgat ttttttctaa accgtggaat
    10381 atttcggata tcctttt
//
