LOCUS       sequence-416476-        4002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC36A2_STOP
FEATURES             Location/Qualifiers
     source          1..4002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2127..2226)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2243..2262)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2244..2262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2267..2283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2267..2283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2385..2404)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2396..3205
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2463..2482)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3352..3940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3841..3860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgagcg tgaccaagtc cacagaggga ccacagggag cagtggccat
      721 caagctggac ctgatgtctc cccctgagag cgccaagaag ctggagaata aggactccac
      781 cttcctggat gagagcccct ccgagtctgc cggcctgaag aagaccaagg gcatcacagt
      841 gtttcaggcc ctgatccacc tggtgaaggg caatatggga acaggaatcc tgggactgcc
      901 tctggcagtg aagaacgcag gcatcctgat gggcccactg tctctgctgg tcatgggctt
      961 catcgcctgc cactgtatgc acatcctggt gaagtgcgcc cagaggttct gtaagcgcct
     1021 gaataagcct tttatggact acggcgatac cgtgatgcac ggcctggagg ccaacccaaa
     1081 tgcctggctg cagaaccacg cacactgggg ccggcacatc gtgagcttct ttctgatcat
     1141 cacacagctg ggcttctgct gcgtgtacat cgtgtttctg gccgacaacc tgaagcaggt
     1201 ggtggaggcc gtgaattcca ccacaaacaa ttgctactct aacgagaccg tgatcctgac
     1261 cccaacaatg gattctaggc tgtatatgct gagcttcctg ccctttctgg tgctgctggt
     1321 gctgatccgg aatctgagaa tcctgaccat cttctccatg ctggccaaca tctccatgct
     1381 ggtgtctctg gtcatcatca tccagtacat cacacaggag atccctgacc caagcaggct
     1441 gccactggtg gcatcctgga agacctatcc cctgttcttt ggcacagcca tcttcagctt
     1501 tgagtccatc ggcgtggtgc tgcccctgga gaacaagatg aagaatgccc ggcacttccc
     1561 tgccatcctg agcctgggca tgtccatcgt gacctctctg tacatcggca tggccgccct
     1621 gggctatctg agatttggcg acgatatcaa ggccagcatc tccctgaacc tgcccaattg
     1681 ctggctgtac cagagcgtga agctgctgta tatcgccggc atcctgtgca catacgccct
     1741 gcagttctat gtgcccgccg agatcatcat cccttttgcc atctctcggg tgagcaccag
     1801 atgggcactg ccactggacc tgtctatcag gctggtcatg gtgtgcctga catgtctgct
     1861 ggccatcctg atccctcgcc tggacctggt catcagcctg gtgggatctg tgagcggaac
     1921 cgccctggcc ctgatcatcc caccactgct ggaggtgacc acattctaca gcgagggcat
     1981 gtcccctctg accatcttta aggatgccct gatctccatc ctgggcttcg tgggctttgt
     2041 ggtgggcaca tatcaggccc tggacgagct gctgaagtcc gaggattctc acccattctc
     2101 caactctacc acatttgtga gataagaccc agctttcttg tacaaagttg gcattataag
     2161 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2221 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2281 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2341 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2401 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2461 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2521 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2581 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2641 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     2701 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2761 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2821 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2881 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2941 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     3001 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     3061 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3121 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     3181 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     3241 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     3301 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3361 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3421 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3481 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3541 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3601 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3661 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3721 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3781 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3841 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3901 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3961 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
