LOCUS       sequence-416480-        3969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC38A5_STOP
FEATURES             Location/Qualifiers
     source          1..3969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2094..2193)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2210..2229)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2211..2229)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2234..2250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2234..2250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2352..2371)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2363..3172
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2430..2449)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3319..3907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3808..3827
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggagc tgcaggaccc caagatgaac ggcgccctgc ctagcgatgc
      721 agtgggatac aggcaggaga gagagggctt cctgccatct aggggaccag cacctggcag
      781 caagcctgtg cagttcatgg actttgaggg caagacctcc ttcggcatga gcgtgttcaa
      841 cctgagcaat gccatcatgg gatccggaat cctgggactg gcatatgcaa tggcacacac
      901 cggcgtgatc ttctttctgg ccctgctgct gtgcatcgcc ctgctgagct cctactccat
      961 ccacctgctg ctgacatgtg ccggcatcgc cggcatcagg gcatatgagc agctgggaca
     1021 gagggccttc ggccctgcag gcaaggtggt ggtggccacc gtgatctgcc tgcacaacgt
     1081 gggcgccatg tctagctacc tgtttatcat caagagcgag ctgccactgg tcatcggcac
     1141 attcctgtat atggaccccg agggcgattg gtttctgaag ggcaatctgc tgatcatcat
     1201 cgtgtccgtg ctgatcatcc tgcccctggc cctgatgaag cacctgggct acctgggcta
     1261 tacctccggc ctgtctctga catgcatgct gttctttctg gtgagcgtga tctacaagaa
     1321 gttccagctg ggctgtgcca tcggccacaa cgagacagcc atggagtctg aggccctggt
     1381 gggactgcct agccagggcc tgaattcctc ttgtgaggcc cagatgttta ccgtggatag
     1441 ccagatgtcc tacacagtgc ctatcatggc cttcgccttc gtgtgccacc cagaggtgct
     1501 gcccatctat accgagctgt gccggccatc caagcggaga atgcaggcag tggcaaacgt
     1561 gagcatcgga gccatgttct gcatgtacgg cctgaccgcc acattcggct acctgacctt
     1621 ctacagctcc gtgaaggccg agatgctgca catgtattct cagaaggacc cactgatcct
     1681 gtgcgtgcgg ctggccgtgc tgctggccgt gaccctgaca gtgcctgtgg tgctgtttcc
     1741 aatcaggcgc gccctgcagc agctgctgtt cccaggcaag gccttttcct ggccaaggca
     1801 cgtggcaatc gccctgatcc tgctggtgct ggtgaacgtg ctggtcatct gcgtgcccac
     1861 catcagagat atcttcggcg tgatcggctc tacaagcgcc ccttccctga tcttcatcct
     1921 gccatctatc ttttacctga ggatcgtgcc cagcgaggtg gagccttttc tgtcctggcc
     1981 aaagatccag gccctgtgct tcggcgtgct gggcgtgctg tttatggccg tgagcctggg
     2041 cttcatgttt gccaattggg ccaccggcca gtctcgcatg agcggccact aagacccagc
     2101 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     2161 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     2221 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     2281 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     2341 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     2401 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     2461 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     2521 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2581 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2641 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     2701 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2761 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2821 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2881 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2941 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     3001 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     3061 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     3121 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     3181 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     3241 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     3301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3421 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3961 tcacatgtt
//
