LOCUS       sequence-416498-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase vector containing 3'UTR for mouse Il13 with mutated DRACH
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Leoni C, Bataclan M, Ito-Kureha T, Heissmeyer V, Monticelli S
  TITLE     The mRNA methyltransferase Mettl3 modulates cytokine mRNA stability 
            and limits functional responses in mast cells.
  JOURNAL   Nat Commun. 2023 Jun 29;14(1):3862. doi: 10.1038/s41467-023-39614-y.
  PUBMED    37386028
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-29"; volume: "14(1):3862. doi"; pages: " 
            10.1038/s41467-023-39614-y"
COMMENT     Sequence Label: pmirGLO-3'UTR mouse Il13 mutDRACH
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    115..236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(126..145)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     180..199
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        481..838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        481..672
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(504..524)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      689..824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      883..892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             889..1821
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1873..2664
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1924..1943)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2534..2553
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2728..2776
                     /note="synthetic polyadenylation signal"
     promoter        2932..3036
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3037..3105,3106..3897)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3255..3274)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(4135..4154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4763..5046
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        5103..5602
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5138..5161)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5535..5554
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      5643..5652
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5649..7301
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    8117..8135
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    8161..8179
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    8185..8203
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    8244..8262
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    8268..8286
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
       61 cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat
      121 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      181 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      241 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa
      301 gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca
      361 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
      421 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
      481 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      841 gcttgattct tctgacacaa cagtctcgaa ccaaaggctg gagccaccat ggcttccaag
      901 gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc
      961 aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag
     1021 aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg
     1081 cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc
     1141 ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg
     1201 ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt
     1261 ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag
     1321 agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc
     1381 ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc
     1441 atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag
     1501 ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct
     1561 ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt
     1621 cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac
     1681 gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc
     1741 cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag
     1801 cgcgtgctga agaacgagca gaccggtggt gggagcggag gtggcggatc aggtggcgga
     1861 ggctccggag ggattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1921 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1981 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     2041 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     2101 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2161 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     2221 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     2281 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     2341 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     2401 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     2461 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     2521 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     2581 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     2641 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     2701 cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
     2761 gttggttttt tgtgtgaatc gatagcgata aggatcctct ttgcgcttgc gttttccctt
     2821 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct
     2881 ttctacgtaa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2941 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3001 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3061 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3121 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3181 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3241 actttcaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3301 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3361 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3421 gataacactg cggccaactt acttctgaca actatcggag gaccgaagga gctaaccgct
     3481 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3541 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3601 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3661 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3721 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3781 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3841 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaattc
     3901 gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt
     3961 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4021 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4081 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4141 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4201 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4261 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4321 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4381 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4441 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     4501 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4561 gatccggcaa acaaaaaaac caccgctacc agcggtggtt tttttgtttg caagcagcag
     4621 attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac
     4681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4741 ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct
     4801 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
     4861 gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag
     4921 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
     4981 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc
     5041 catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatcta
     5101 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     5161 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     5221 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     5281 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     5341 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     5401 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     5461 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     5521 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     5581 ttcctcatct ccgggccttt cgacctgcag cccaagcttg gcaatccggt actgttggta
     5641 aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg
     5701 aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg
     5761 gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg
     5821 agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga
     5881 tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca
     5941 tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca
     6001 tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca
     6061 acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact
     6121 accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg
     6181 agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca
     6241 gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc
     6301 gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc
     6361 tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct
     6421 gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc
     6481 aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga
     6541 gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc
     6601 cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc
     6661 agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca
     6721 agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca
     6781 ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca
     6841 tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc
     6901 tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc
     6961 tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc
     7021 tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg
     7081 gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga
     7141 tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt
     7201 tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga
     7261 ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta attctagttg tttaaacgag
     7321 ctcgctagcg aattctgagg agagcgcttc cctgggcatc tcagctgtgg taccattttc
     7381 ctttctcaca tcaagctttg ctggggagag gcagggagga gggttgagga ggaagggaga
     7441 tgcctcagct ttggcctcag cctgcactgc ctgcctagtg ctcagggtct cagcctggca
     7501 acacccccac cccaccccca cccccgccgc cccatcccat ccctacagaa aactgcagca
     7561 agaccgtgag tccagcctgt ggcctggtcc acacagggca actgaggcag gcagcagctt
     7621 gagcacattt cttcttgatc ttatttatta tggttgtgtg ttatttaaat gagtctgtca
     7681 gtatcccggt ggggacatgg tttgctgcct atgccctggg ggctccagca ttgaagcagt
     7741 gggctctggg gtccctggca atattactgt atacataact ctgctacctc actgtagcct
     7801 ccaggtctca ccccaggcag gagatgggag gggaggccag agcaacactc ctgtctgcca
     7861 cggcagcaac cagccctcag ccatgaaata acttattgtt ttgttcttat atttaaagta
     7921 ttaaatagct tagcaaagag ttaataatat atggaagaat ggcctgttac actcaaggtg
     7981 atgtgtagtg aatgggggga gggtggtggg tttgtcactg aacaaacttt tcattgactg
     8041 tcaaactaga aaccggaaat aaagatggtg acagattcta gattccctaa gtccaactac
     8101 taaactgggg attcctgggc cctgaagaag ggcccctcga ctaagtccaa ctactaaact
     8161 gggccctgaa gaagggccca tatagggccc tgaagaaggg ccctatcgag gatattatct
     8221 cgactaagtc caactactaa actgggccct gaagaagggc ccatataggg ccctgaagaa
     8281 gggccctatc gaggatatta tctcgagtca gtcgacctgc agg
//
