LOCUS       sequence-416502-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid to express mouse Mettl3 Lambda peptide C-ter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Leoni C, Bataclan M, Ito-Kureha T, Heissmeyer V, Monticelli S
  TITLE     The mRNA methyltransferase Mettl3 modulates cytokine mRNA stability 
            and limits functional responses in mast cells.
  JOURNAL   Nat Commun. 2023 Jun 29;14(1):3862. doi: 10.1038/s41467-023-39614-y.
  PUBMED    37386028
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-29"; volume: "14(1):3862. doi"; pages: " 
            10.1038/s41467-023-39614-y"
COMMENT     Sequence Label: pSCALPS-FLAG-HA-mmuMettl3-LN
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..84)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        69..398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     311..330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        410..789
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        791..989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     944..964
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1007..1187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1234..1359
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1856..2089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2273..2317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2615..2732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2869..3276
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3323..3388
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      3395..3404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3404..3427
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3440..3466
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3440..3466
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5748..6347
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5748..5767)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6244..6264
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6357..6945
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6410..6430)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6828..6839)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7463..7643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7714..8302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7794..7813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8473..9264,9265..9333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9096..9115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9334..9438)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9513..9532)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
       61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
      361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgcgttg acattgatta
      421 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      481 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      541 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      601 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      661 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      721 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      781 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      841 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      901 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      961 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     1021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1201 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1261 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1321 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1381 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1441 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1501 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1561 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1621 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1681 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     1741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaattaacaa
     2221 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2281 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2341 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2401 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2461 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2521 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2581 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2641 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2701 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2761 cagtttggtt aattaactgc agccccgata aaataaaaga ttttatttag tctccagaaa
     2821 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
     2881 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
     2941 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
     3001 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
     3061 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
     3121 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
     3181 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
     3241 ggcgcgccag tcctccgaca gactgagtcg cccgggggtc tagaagcgct ggatccacta
     3301 gtccagtgtg gtggaattca ccatggacgc acaaacacga cgacgtgagc gtcgcgctga
     3361 gaaacaagct caatggaaag ctgcaaacgc ggccgccacc atggactaca aggacgacga
     3421 tgacaagctc gatggaggat acccctacga cgtgcccgac tacgccggag aatcgatggg
     3481 agctaggatg tcggacacgt ggagctctat ccaggcccat aagaaacagc tggactcgct
     3541 tcgcgagaga ttgcagcggc gacggaagca ggactctggg cacttggatt taaggaaccc
     3601 agaagcggca ctgtccccaa ccttccgtag tgatagtccc gtgcctactg cccctacctc
     3661 tagcggccct aagcccagca caacatctgt ggcccctgaa ctagctacag accctgagtt
     3721 agagaagaag ttgctacacc acctctcaga tctggccttg accttgccca ctgatgctgt
     3781 ttccatccgt cttgccatct ctacgccaga tgcacctgcc actcaagatg gggtagaaag
     3841 ccttctccag aaatttgccg cccaggagtt gattgaggta aagcgaggtc tcctccaaga
     3901 tgatgcacat cctactcttg taacttacgc tgaccactcc aagctgtctg ccatgatggg
     3961 ggctgtggca gaaaagaaag gtcttggaga ggtagcaggg accatcgcag ggcagaaacg
     4021 gcgtgcagaa caggatttga ctacagtgac cacctttgcc agctctttag catctggtct
     4081 ggcctcttca gcatcagaac cagctaagga gccggctaag aagtcaagga agcacgctgc
     4141 ctccgatgtt gatctggaga tagaaagcct tttgaaccaa cagtcaacga aagaacagca
     4201 gagcaagaag gtcagtcagg agatcctaga gctattaaat accacaacag ccaaggaaca
     4261 gtccattgtt gaaaagtttc gctctcgagg tcgggcccag gtgcaagaat tttgtgatta
     4321 tgggaccaag gaagagtgca tgaaagccag tgacgctgac cggccttgtc gcaagctgca
     4381 cttcagacga attatcaata agcacactga tgaatcttta ggtgactgct ctttccttaa
     4441 cacatgtttc cacatggaca cctgcaaata tgttcactat gaaattgatg cttgtgttga
     4501 ttctgagagt cctggcagca aggagcatat gccaagccag gagcttgctc ttacacagag
     4561 tgttgggggt gactccagtg ctgatcgact ctttccacct cagtggatct gttgtgatat
     4621 ccgctacctg gacgtcagta tcttgggcaa atttgcagtt gtgatggctg acccaccttg
     4681 ggatattcac atggagctac cgtatgggac attaacagat gatgagatgc gcaggctcaa
     4741 tataccagtg ctacaggatg acggctttct tttcctctgg gtcacaggaa gggccatgga
     4801 attgggcaga gaatgtctga acctctgggg ttatgaacgg gtggatgaaa tcatctgggt
     4861 gaagactaat cagctgcagc gcatcattag gacgggccgg acgggtcact ggttaaacca
     4921 cgggaaggaa cactgcttgg ttggtgttaa gggaaatcct caaggattca accagggcct
     4981 ggactgcgat gtgattgtag ctgaggttcg ttccaccagt cataaaccag atgaaatata
     5041 tggcatgatt gagagactgt cccctggcac ccgcaagatt gagttatttg gacgaccaca
     5101 caatgtgcag cccaactgga ttactcttgg aaaccaactg gatgggatac acctactaga
     5161 cccagatgtg gttgccaggt ttaagcaaag gtatccggac ggcatcatct ctaaacctaa
     5221 gaatttatag acgcagttaa ctggccagaa ttctcgacct tgactggcgg cgcgaccttg
     5281 aggcctgcgt tcgcctcagt tgccccctct gtgcaatggg gagacgcgcc tcatcgcttg
     5341 acaacggccg aagagccgcc gcgcttccgt ctcccgcgtg cgcgcgccat gctgcccacc
     5401 cccgttccgc actgaccctc ccccgtgccc cgcgtcccgt actgccgccc cgccccgagt
     5461 cccatgccgc agccaccgcg acggagcccg caggcgggaa cctgcctccg cgcgttagcg
     5521 cgcacgcgcg cctcatgtgt cgtccccatc agcgccggct tccgtctata ggccagatgc
     5581 actgtcactc tggcgaagtc gcagacccga ttggccggga cggaggcgcg agaccgggtt
     5641 gcgggcgggg ccgaacgtgg tataaaaggg gcgggaggcc aggctcgtgc cgttttgcag
     5701 acgccaccgc cgaggaaaac cgtgtactat tagccacgcg tgccaccatg accgagtaca
     5761 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     5821 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     5881 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5941 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6001 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6061 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6121 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6181 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6241 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6301 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg atcgctaatc
     6361 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6421 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6481 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6541 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6601 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6661 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6721 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     6781 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6841 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6901 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcttaat cgcgtcgaga
     6961 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     7021 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     7081 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7141 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     7201 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     7261 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     7321 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     7381 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     7441 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7501 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7561 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7621 gtcagtgtgg aaaatctcta gcagggcccg tttcatgtga gcaaaaggcc agcaaaaggc
     7681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8221 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8281 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8341 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8401 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8461 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8521 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8581 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8641 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8701 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8761 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8821 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8881 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8941 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9001 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9061 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9121 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9181 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9241 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9301 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattggag
     9361 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9421 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     9481 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     9541 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     9601 taagctacaa caaggcaagg cttgaccgac aattgcatga ag
//
