LOCUS       sequence-416511-       12488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PE2max that co-express dnMLH1 and p53DD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12488)
  AUTHORS   Park JC, Kim YJ, Hwang GH, Kang CY, Bae S, Cha HJ
  TITLE     Enhancing genome editing in hPSCs through dual inhibition of DNA 
            damage response and repair pathways.
  JOURNAL   Nat Commun. 2024 May 11;15(1):4002. doi: 10.1038/s41467-024-48111-9.
  PUBMED    38734692
REFERENCE   2  (bases 1 to 12488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-11"; volume: "15(1):4002. doi"; pages: " 
            10.1038/s41467-024-48111-9"
COMMENT     Sequence Label: pCMV-PE4stem
FEATURES             Location/Qualifiers
     source          1..12488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..58)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        230..609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        610..813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     764..784
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             923..943
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             944..5044
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5102..5122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7223..7243
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7253..7279
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7289..7345
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9602..9655
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9981..9998
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10021..10038)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10027..10251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10322..10338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10322..10338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10335..10357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10346..10362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10370..10376,10377..10394,10395..10400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10415..10436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10553..10570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10724..11312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10804..10823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11483..12274,12275..12343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12106..12125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12344..12448)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggagatcgat ctcccgatcc cctagggtct actctcagta caatctgctc tgatgccgca
       61 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      121 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      181 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      241 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      301 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      361 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      421 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      481 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      541 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      601 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      661 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      721 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      781 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      841 agatccgcgg ccgctaatac gactcactat agggagagcc gccaccatga aacggacagc
      901 cgacggaagc gagttcgagt caccaaagaa gaagcggaaa gtcgacaaga agtacagcat
      961 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
     1021 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
     1081 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
     1141 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
     1201 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
     1261 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
     1321 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
     1381 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
     1441 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
     1501 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
     1561 cgccaaggcc atcctgtctg ccagactgag caagagcaga aagctggaaa atctgatcgc
     1621 ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg
     1681 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
     1741 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
     1801 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
     1861 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
     1921 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
     1981 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc
     2041 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
     2101 ggaactgctc gtgaagctga agagagagga cctgctgcgg aagcagcgga ccttcgacaa
     2161 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
     2221 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
     2281 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
     2341 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc
     2401 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
     2461 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt
     2521 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc
     2581 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
     2641 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
     2701 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
     2761 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
     2821 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
     2881 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
     2941 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
     3001 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
     3061 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
     3121 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
     3181 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
     3241 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
     3301 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
     3361 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
     3421 actggacatc aaccggctgt ccgactacga tgtggacgct atcgtgcctc agagctttct
     3481 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag
     3541 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct
     3601 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg
     3661 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
     3721 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
     3781 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
     3841 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
     3901 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
     3961 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
     4021 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
     4081 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
     4141 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
     4201 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
     4261 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
     4321 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
     4381 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
     4441 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
     4501 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
     4561 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
     4621 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
     4681 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
     4741 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
     4801 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
     4861 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
     4921 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
     4981 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
     5041 tgactccggc ggaagctctg gtggcagcaa gcggaccgcc gacggctctg aattcgagag
     5101 ccctaagaag aaaagaaagg tgagcggagg ctctagcggc ggaagcaccc tgaacattga
     5161 agacgagtat agactgcatg aaacaagcaa ggaacccgac gtgtccctgg gctccacctg
     5221 gctgtccgac tttccccagg cctgggccga gacaggagga atgggcctgg ccgtgcggca
     5281 ggcacccctg atcatccctc tgaaggccac ctctacaccc gtgagcatca agcagtaccc
     5341 tatgtctcag gaggccagac tgggcatcaa gcctcacatc cagaggctgc tggaccaggg
     5401 catcctggtg ccatgccaga gcccctggaa cacaccactg ctgcccgtga agaagccagg
     5461 caccaatgac tatagacccg tgcaggatct gagagaggtg aacaagaggg tggaggatat
     5521 ccaccccacc gtgcccaacc cttacaatct gctgtccggc ctgccccctt ctcaccagtg
     5581 gtatacagtg ctggacctga aggatgcctt cttttgtctg agactgcacc ctaccagcca
     5641 gccactgttc gcctttgagt ggagggaccc tgagatgggc atctctggcc agctgacctg
     5701 gacacgcctg cctcagggct tcaagaatag cccaacactg tttaacgagg ccctgcaccg
     5761 cgacctggca gatttccgga tccagcaccc agatctgatc ctgctgcagt acgtggacga
     5821 tctgctgctg gccgccacca gcgagctgga ttgccagcag ggaacacgcg ccctgctgca
     5881 gaccctggga aacctgggat atagggcatc cgccaagaag gcccagatct gtcagaagca
     5941 ggtgaagtac ctgggctatc tgctgaagga gggccagaga tggctgacag aggccaggaa
     6001 ggagacagtg atgggccagc caacacccaa gaccccaaga cagctgaggg agttcctggg
     6061 caaagcagga ttttgcaggc tgttcatccc aggattcgca gagatggcag cacctctgta
     6121 cccactgacc aagccgggca ccctgtttaa ttggggccct gaccagcaga aggcctatca
     6181 ggagatcaag caggccctgc tgacagcacc agccctgggc ctgccagacc tgaccaagcc
     6241 tttcgagctg tttgtggatg agaagcaggg ctacgccaag ggcgtgctga cccagaagct
     6301 gggaccatgg agacggcccg tggcctatct gtccaagaag ctggacccag tggcagcagg
     6361 atggccacca tgcctgagga tggtggcagc aatcgccgtg ctgacaaagg atgccggcaa
     6421 gctgaccatg ggacagccac tggtcatcct ggcaccacac gcagtggagg ccctggtgaa
     6481 gcagcctcca gatcgctggc tgtctaacgc ccggatgaca cactaccagg ccctgctgct
     6541 ggacaccgat cgcgtgcagt ttggccctgt ggtggccctg aatccagcca ccctgctgcc
     6601 tctgccagag gagggcctgc agcacaactg tctggacatc ctggcagagg cacacggaac
     6661 aaggccagac ctgaccgatc agcccctgcc tgacgccgat cacacatggt ataccgatgg
     6721 aagctccctg ctgcaggagg gccagaggaa ggcaggagca gcagtgacca cagagacaga
     6781 agtgatctgg gccaaggccc tgccagcagg cacatccgcc cagcgggccg agctgatcgc
     6841 cctgacccag gccctgaaga tggccgaggg caagaagctg aacgtgtaca cagactccag
     6901 atatgccttc gccaccgcac acatccacgg agagatctac aggcgccggg gctggctgac
     6961 ctctgagggc aaggagatca agaacaagga tgagatcctg gccctgctga aggccctgtt
     7021 tctgcccaag cggctgagca tcatccactg tcctggacac cagaagggac actccgccga
     7081 ggcaaggggc aatcggatgg ccgaccaggc cgccagaaag gctgctatta ctgaaactcc
     7141 cgacacttcc actctgctga ttgaaaactc ctccccttct ggcggctcaa aaagaaccgc
     7201 cgacggcagc gaattcgagt ctcccaagaa gaagaggaaa gtcggctctg gccctgccgc
     7261 taagagagtg aagctggacg gaagcggagc tactaacttc agcctgctga agcaggctgg
     7321 agacgtggag gagaaccctg gacctagctt cgttgctgga gtcatccgga gactggacga
     7381 gacagtggtg aacagaattg ccgccggcga ggtgatccag agacctgcca atgcaataaa
     7441 ggagatgatc gagaactgtc tggacgccaa gtccacaagc attcaggtga tcgtgaagga
     7501 gggcggactg aagctgatcc agatccaaga caacggcaca ggcatcagaa aggaagatct
     7561 ggacatcgtg tgtgaacggt tcaccacatc taagctgcag tcttttgagg atctggcctc
     7621 tatcagtacc tacggcttca gaggcgaggc cctggccagc atcagccacg tggcccatgt
     7681 gaccatcacc accaaaaccg ccgacggcaa atgcgcttat cgcgctagct acagcgacgg
     7741 caagctgaaa gccccgccaa agccttgcgc cggcaaccag ggtacacaga taacagtgga
     7801 ggatctgttc tacaacatcg ccacccggag aaaggccctg aaaaatccca gcgaggagta
     7861 cggcaagatc ctggaagtcg tcggcagata ctccgtgcac aacgccggaa tcagctttag
     7921 cgtaaagaag cagggagaaa ccgtggccga tgtgcgcacc ctgccaaatg ccagcaccgt
     7981 ggataacatc agaagcattt tcggaaatgc cgtgtccaga gaactgatcg agatcggctg
     8041 cgaagataag accctggctt ttaagatgaa cggctacatc agcaacgcca attactctgt
     8101 gaagaagtgc atctttcttc tgttcatcaa ccacagactg gtggaaagca ccagcctgcg
     8161 gaaagccatc gagacagtgt acgccgccta cctgcctaag aacacccacc ccttcctgta
     8221 cctgagcctc gagatcagcc ctcagaacgt ggacgtcaat gtgcatccta caaagcacga
     8281 ggtgcacttc ctgcacgagg aaagcatcct ggaaagagtg cagcagcaca ttgagagcaa
     8341 gctgctgggc tctaacagca gcagaatgta cttcacacag accctgctgc ctggcctggc
     8401 cggcccctca ggcgaaatgg ttaagtccac aacctctctg acctcatcca gcaccagcgg
     8461 ttcttccgat aaggtgtacg cccaccagat ggtgcggacc gactctcggg agcagaagct
     8521 ggacgccttt ctgcaacctc tgagcaaacc tctgagctct cagcctcagg ccatcgtgac
     8581 cgaggacaag acagatatct cctccggccg tgccagacag caggacgaag aaatgctcga
     8641 gctgccagct cctgccgagg tggccgccaa gaaccagagc ctggagggag ataccacaaa
     8701 gggcaccagc gaaatgagcg agaagcgggg ccctacctcc agcaacccca gaaaacggca
     8761 ccgggaggac agcgacgtgg aaatggtgga ggacgacagc agaaaggaaa tgacagccgc
     8821 ttgtacccct agaagaagaa tcatcaacct gacctccgtg ctgagcctgc aggaggagat
     8881 caacgagcag ggccacgagg tgctgagaga gatgctgcac aatcacagct tcgtgggctg
     8941 cgtgaaccct caatgggccc tggctcagca tcaaacaaag ctgtacctgc tgaacaccac
     9001 caagctgagc gaagagctgt tctaccagat cctcatctac gacttcgcca acttcggcgt
     9061 gctacgcctg agcgagcccg cccctctgtt tgacctggcc atgctggctc tggatagccc
     9121 agaaagcggc tggacagaag aggacggacc taaagagggg ctggctgaat acatcgtgga
     9181 gttcctgaag aaaaaggccg agatgctggc cgactacttt tctctggaaa tcgacgagga
     9241 aggcaacctg atcggcctgc ctctgctgat cgataactac gtgcctcccc tggaaggcct
     9301 gcccatcttc atcctgagac tggctacaga ggtgaactgg gacgaggaaa aggaatgctt
     9361 cgagtctctg agcaaggagt gcgccatgtt ctatagcatc agaaaacagt acatctctga
     9421 agagagcact ctgtctggcc agcagagtga agtgcccgga agcatcccca acagctggaa
     9481 gtggaccgtg gaacacatcg tgtacaaggc cctgcggagc cacattctcc ctcctaagca
     9541 cttcaccgag gacggcaaca tcctgcagct ggccaacctg cccgaccttt ataaggtttt
     9601 cgaaggacgg ggctccctcc tgacctgtgg cgatgtggaa gagaaccccg gccccatggc
     9661 tagcatgact ggtggacagc aaatgggatc ccccccaggg agcactaagc gagcactgcc
     9721 caacaacacc agctcctctc cccagccaaa gaagaaacca ctggatggag aatatttcac
     9781 ccttcagatc cgtgggcgtg agcgcttcga gatgttccga gagctgaatg aggccttgga
     9841 actcaaggat gcccaggctg ggaaggagcc aggggggagc agggctcact ccagccacct
     9901 gaagtccaaa aagggtcagt ctacctcccg ccataaaaaa cacatgttca agacagaagg
     9961 gcctgactca gactgacggt catcatcacc atcaccattg agtttaaacc cgctgatcag
    10021 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    10081 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgagaaa attgcatcgc
    10141 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    10201 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    10261 cggaaagaac cagctggggc tcgataccgt cgacctctag ctagagcttg gcgtaatcat
    10321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10381 ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc acattaattg
    10441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11161 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11341 ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    12001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    12061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    12121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    12181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    12241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    12301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    12361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    12421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    12481 acggatcg
//
