LOCUS       sequence-416523-        9672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AncBE4max that co-expresses UGI and p53DD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Park JC, Kim YJ, Hwang GH, Kang CY, Bae S, Cha HJ
  TITLE     Enhancing genome editing in hPSCs through dual inhibition of DNA 
            damage response and repair pathways.
  JOURNAL   Nat Commun. 2024 May 11;15(1):4002. doi: 10.1038/s41467-024-48111-9.
  PUBMED    38734692
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-11"; volume: "15(1):4002. doi"; pages: " 
            10.1038/s41467-024-48111-9"
COMMENT     Sequence Label: pCMV-AncBE4stem
FEATURES             Location/Qualifiers
     source          1..9672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2121..2369
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             2400..2648
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             2691..2711
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2712..2765
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3084..3140
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3141..3389
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             3402..3422
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3431..3448
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3471..3488)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3477..3701
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3772..3788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3772..3788)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3785..3807)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3796..3812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3820..3826,3827..3844,3845..3850))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3865..3886
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4003..4020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4174..4762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4254..4273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4933..5724,5725..5793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5556..5575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5794..5898)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5977..5996)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6168..6547
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6548..6751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6702..6722
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6793..6812
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6793..6811
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6861..6881
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7662..7685,7686..7688,7689..9672,1..2090)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(7662..9672,1..2090)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa
       61 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc
      121 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg
      181 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg
      241 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga
      301 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg
      361 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt
      421 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc
      481 ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca
      541 tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct
      601 ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga
      661 ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg
      721 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg
      781 cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg
      841 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt
      901 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctaaacgccg
      961 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg
     1021 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg
     1081 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc
     1141 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga
     1201 tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag
     1261 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc
     1321 ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg
     1381 gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg
     1441 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa
     1501 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa
     1561 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga
     1621 gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat
     1681 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata
     1741 atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc
     1801 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt
     1861 ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc
     1921 tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg
     1981 accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca
     2041 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggtgac agcggcggga
     2101 gcggcgggag cggggggagc actaatctga gcgacatcat tgagaaggag actgggaaac
     2161 agctggtcat tcaggagtcc atcctgatgc tgcctgagga ggtggaggaa gtgatcggca
     2221 acaagccaga gtctgacatc ctggtgcaca ccgcctacga cgagtccaca gatgagaatg
     2281 tgatgctgct gacctctgac gcccccgagt ataagccttg ggccctggtc atccaggatt
     2341 ctaacggcga gaataagatc aagatgctga gcggaggatc cggaggatct ggaggcagca
     2401 ccaacctgtc tgacatcatc gagaaggaga caggcaagca gctggtcatc caggagagca
     2461 tcctgatgct gcccgaagaa gtcgaagaag tgatcggaaa caagcctgag agcgatatcc
     2521 tggtccatac cgcctacgac gagagtaccg acgaaaatgt gatgctgctg acatccgacg
     2581 ccccagagta taagccctgg gctctggtca tccaggattc caacggagag aacaaaatca
     2641 aaatgctgtc tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga
     2701 aaaggaaagt cgaaggacgg ggctccctcc tgacctgtgg cgatgtggaa gagaaccccg
     2761 gccccatggc tagcatgact ggtggacagc aaatgggatc ccccccaggg agcactaagc
     2821 gagcactgcc caacaacacc agctcctctc cccagccaaa gaagaaacca ctggatggag
     2881 aatatttcac ccttcagatc cgtgggcgtg agcgcttcga gatgttccga gagctgaatg
     2941 aggccttgga actcaaggat gcccaggctg ggaaggagcc aggggggagc agggctcact
     3001 ccagccacct gaagtccaaa aagggtcagt ctacctcccg ccataaaaaa cacatgttca
     3061 agacagaagg gcctgactca gacgctacta acttcagcct gctgaagcag gctggagacg
     3121 tggaggagaa ccctggacct actaatctgt cagatattat tgaaaaggag accggtaagc
     3181 aactggttat ccaggaatcc atcctcatgc tcccagagga ggtggaagaa gtcattggga
     3241 acaagccgga aagcgatata ctcgtgcaca ccgcctacga cgagagcacc gacgagaatg
     3301 tcatgcttct gactagcgac gcccctgaat acaagccttg ggctctggtc atacaggata
     3361 gcaacggtga gaacaagatt aagatgctct ctggtggttc tcccaagaag aagaggaaag
     3421 tctaaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     3481 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3541 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3601 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3661 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3721 cagctggggc tcgataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     3781 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3841 aaagtgtaaa gcctaggatg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3901 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3961 cgcgggaaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4021 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4081 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4141 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4201 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4261 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4321 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4381 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4441 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4501 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4561 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4621 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4681 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4741 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacactca
     4801 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4861 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4921 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4981 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5041 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5101 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5161 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5221 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5281 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5341 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5401 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5461 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5521 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5581 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5641 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5701 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5761 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5821 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5881 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     5941 agatcgatct cccgatcccc tagggtcgac tctcagtaca atctgctctg atgccgcata
     6001 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     6061 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     6121 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     6181 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     6241 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     6301 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     6361 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     6421 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     6481 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     6541 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     6601 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     6661 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     6721 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagag
     6781 atccgcggcc gctaatacga ctcactatag ggagagccgc caccatgaaa cggacagccg
     6841 acggaagcga gttcgagtca ccaaagaaga agcggaaagt cagcagtgaa accggaccag
     6901 tggcagtgga cccaaccctg aggagacgga ttgagcccca tgaatttgaa gtgttctttg
     6961 acccaaggga gctgaggaag gagacatgcc tgctgtacga gatcaagtgg ggcacaagcc
     7021 acaagatctg gcgccacagc tccaagaaca ccacaaagca cgtggaagtg aatttcatcg
     7081 agaagtttac ctccgagcgg cacttctgcc cctctaccag ctgttccatc acatggtttc
     7141 tgtcttggag cccttgcggc gagtgttcca aggccatcac cgagttcctg tctcagcacc
     7201 ctaacgtgac cctggtcatc tacgtggccc ggctgtatca ccacatggac cagcagaaca
     7261 ggcagggcct gcgcgatctg gtgaattctg gcgtgaccat ccagatcatg acagccccag
     7321 agtacgacta ttgctggcgg aacttcgtga attatccacc tggcaaggag gcacactggc
     7381 caagataccc acccctgtgg atgaagctgt atgcactgga gctgcacgca ggaatcctgg
     7441 gcctgcctcc atgtctgaat atcctgcgga gaaagcagcc ccagctgaca tttttcacca
     7501 ttgctctgca gtcttgtcac tatcagcggc tgcctcctca tattctgtgg gctacaggcc
     7561 tgaagtctgg aggatctagc ggaggatcct ctggcagcga gacaccagga acaagcgagt
     7621 cagcaacacc agagagcagt ggcggcagca gcggcggcag cgacaagaag tacagcatcg
     7681 gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
     7741 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
     7801 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
     7861 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
     7921 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
     7981 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     8041 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     8101 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     8161 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     8221 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     8281 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
     8341 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
     8401 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     8461 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     8521 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     8581 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     8641 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     8701 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     8761 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     8821 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     8881 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     8941 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     9001 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     9061 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     9121 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     9181 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     9241 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     9301 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     9361 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     9421 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     9481 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     9541 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     9601 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     9661 acggcatccg gg
//
