LOCUS       sequence-416547-       10656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express human METTL3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Leoni C, Bataclan M, Ito-Kureha T, Heissmeyer V, Monticelli S
  TITLE     The mRNA methyltransferase Mettl3 modulates cytokine mRNA stability 
            and limits functional responses in mast cells.
  JOURNAL   Nat Commun. 2023 Jun 29;14(1):3862. doi: 10.1038/s41467-023-39614-y.
  PUBMED    37386028
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-29"; volume: "14(1):3862. doi"; pages: " 
            10.1038/s41467-023-39614-y"
COMMENT     Sequence Label: plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen
FEATURES             Location/Qualifiers
     source          1..10656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3556..3579
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5342..5914
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5365..5916
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5509..5526)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5916..6611
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    6625..7213
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6678..6698)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7096..7107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7420..8053
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8181..8197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8181..8197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8194..8216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8205..8221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8229..8235,8236..8253,8254..8259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8274..8295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8412..8429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8583..9171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8663..8682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9342..10133,10134..10202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9965..9984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10203..10307)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10355..10489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10392..10411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10446..10465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 ctcgagacta gtatggatta caaggatgac gatgacaagt cggacacgtg gagctctatc
     3601 caggcccaca agaagcagct ggactctctg cgggagaggc tgcagcggag gcggaagcag
     3661 gactcggggc acttggatct acggaatcca gaggcagcat tgtctccaac cttccgtagt
     3721 gacagcccag tgcctactgc acccacctct ggtggcccta agcccagcac agcttcagca
     3781 gttcctgaat tagctacaga tcctgagtta gagaagaagt tgctacacca cctctctgat
     3841 ctggccttaa cattgcccac tgatgctgtg tccatctgtc ttgccatctc cacgccagat
     3901 gctcctgcca ctcaagatgg ggtagaaagc ctcctgcaga agtttgcagc tcaggagttg
     3961 attgaggtaa agcgaggtct cctacaagat gatgcacatc ctactcttgt aacctatgct
     4021 gaccattcca agctctctgc catgatgggt gctgtggcag aaaagaaggg ccctggggag
     4081 gtagcaggga ctgtcacagg gcagaagcgg cgtgcagaac aggactcgac tacagtagct
     4141 gcctttgcca gttcgttagt ctctggtctg aactcttcag catcggaacc agcaaaggag
     4201 ccagccaaga aatcaaggaa acatgctgcc tcagatgttg atctggagat agagagcctt
     4261 ctgaaccaac agtccactaa ggaacaacag agcaagaagg tcagtcagga gatcctagag
     4321 ctattaaata ctacaacagc caaggaacaa tccattgttg aaaaatttcg ctctcgaggt
     4381 cgggcccaag tgcaagaatt ctgtgactat ggaaccaagg aggagtgcat gaaagccagt
     4441 gatgctgatc gaccctgtcg caagctgcac ttcagacgaa ttatcaataa acacactgat
     4501 gagtctttag gtgactgctc tttccttaat acatgtttcc acatggatac ctgcaagtat
     4561 gttcactatg aaattgatgc ttgcatggat tctgaggccc ctggcagcaa agaccacacg
     4621 ccaagccagg agcttgctct tacacagagt gtcggaggtg attccagtgc agaccgactc
     4681 ttcccacctc agtggatctg ttgtgatatc cgctacctgg acgtcagtat cttgggcaag
     4741 tttgcagttg tgatggctga cccaccctgg gatattcaca tggaactgcc ctatgggacc
     4801 ctgacagatg atgagatgcg caggctcaac atacccgtac tacaggatga tggctttctc
     4861 ttcctctggg tcacaggcag ggccatggag ttggggagag aatgtctaaa tctctggggg
     4921 tatgaacggg tagatgaaat tatttgggtg aagacaaatc aactgcaacg catcattcgg
     4981 acaggccgta caggtcactg gttgaaccat gggaaggaac actgcttggt tggtgtcaaa
     5041 ggaaatcccc aaggcttcaa ccagggtctg gattgtgatg tgatcgtagc tgaggttcgt
     5101 tccaccagtc ataaaccaga tgaaatctat ggcatgattg aaagactatc tcctggcact
     5161 cgcaagattg agttatttgg acgaccacac aatgtgcaac ccaactggat cacccttgga
     5221 aaccaactgg atgggatcca cctactagac ccagatgtgg ttgcacggtt caagcaaagg
     5281 tacccagatg gtatcatctc taaacctaag aatttatagt ctagagcggc cgcggatccc
     5341 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     5401 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     5461 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     5521 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     5581 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     5641 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5701 acgttgtgag ttggatagtt gtggaaagag tcaaatggct cacctcaagc gtattcaaca
     5761 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5821 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
     5881 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc ccagtccaag cacggcctga
     5941 ccaaggagat gaccatgaag taccgcatgg agggctgcgt ggacggccac aagttcgtga
     6001 tcaccggcga gggcatcggc taccccttca agggcaagca ggccatcaac ctgtgcgtgg
     6061 tggagggcgg ccccttgccc ttcgccgagg acatcttgtc cgccgccttc atgtacggca
     6121 accgcgtgtt caccgagtac ccccaggaca tcgtcgacta cttcaagaac tcctgccccg
     6181 ccggctacac ctgggaccgc tccttcctgt tcgaggacgg cgccgtgtgc atctgcaacg
     6241 ccgacatcac cgtgagcgtg gaggagaact gcatgtacca cgagtccaag ttctacggcg
     6301 tgaacttccc cgccgacggc cccgtgatga agaagatgac cgacaactgg gagccctcct
     6361 gcgagaagat catccccgtg cccaagcagg gcatcttgaa gggcgacgtg agcatgtacc
     6421 tgctgctgaa ggacggtggc cgcttgcgct gccagttcga caccgtgtac aaggccaagt
     6481 ccgtgccccg caagatgccc gactggcact tcatccagca caagctgacc cgcgaggacc
     6541 gcagcgacgc caagaaccag aagtggcacc tgaccgagca cgccatcgcc tccggctccg
     6601 ccttgccctg aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     6661 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6721 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6781 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     6841 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     6901 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     6961 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     7021 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7081 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7141 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7201 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     7261 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7321 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7381 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     7441 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7501 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     7561 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     7621 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     7681 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     7741 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     7801 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     7861 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7921 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7981 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8041 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8101 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     8161 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8221 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8281 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8341 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8761 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9001 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9301 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9361 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9421 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9481 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9541 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9601 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9661 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9721 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9781 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9841 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9901 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9961 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10021 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10081 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10141 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10201 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10261 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10321 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    10381 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10441 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10501 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    10561 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    10621 ttgtatttgt taaatatgta ctacaaactt agtagt
//
