LOCUS       sequence-416551-        7186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express waaV and waaL under the control of PBAD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Allard N, Collette A, Paquette J, Rodrigue S, Cote JP
  TITLE     Systematic investigation of recipient cell genetic requirements 
            reveals important surface receptors for conjugative transfer of 
            IncI2 plasmids.
  JOURNAL   Commun Biol. 2023 Nov 16;6(1):1172. doi: 10.1038/s42003-023-05534-2.
  PUBMED    37973843
REFERENCE   2  (bases 1 to 7186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-11-16"; volume: "6(1):1172. doi"; pages: " 
            10.1038/s42003-023-05534-2"
COMMENT     Sequence Label: pWaaVL
FEATURES             Location/Qualifiers
     source          1..7186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..40)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      193..279
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      371..398
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        417..508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(509..577,578..1369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(727..746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1411..1866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1498..1517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1708..1729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2694..2711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2828..3373)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2908..2927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3449..3468
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(3648..4526)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4553..4837
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4760..4779
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
       61 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
      121 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
      181 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      241 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      301 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      361 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
      421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      721 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1021 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     1081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1381 agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg
     1441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     1501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     1561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     1621 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     1681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     1741 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     1801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     1861 caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa accaccgcta ccagcggtgg
     2041 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2101 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2161 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtcagg catttgagaa
     2221 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
     2281 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg
     2341 ccattcatcc gcttattatc acttattcag gcgtagcaac caggcgttta agggcaccaa
     2401 taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
     2461 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     2521 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggctagcgg agtgtatact
     2581 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     2641 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     2701 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     2761 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     2821 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     2881 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     2941 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     3001 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     3061 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     3121 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     3181 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     3241 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     3301 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     3361 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctagg atcatgagcc
     3421 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     3481 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt
     3541 tgacagctta tcatcgatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa
     3601 accctatgct actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg
     3661 acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg
     3721 gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg
     3781 acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg
     3841 tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc
     3901 gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg
     3961 tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac
     4021 tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc
     4081 tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa
     4141 tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag
     4201 ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg
     4261 cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca
     4321 cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg
     4381 agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg
     4441 ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc
     4501 aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc
     4561 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta
     4621 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg
     4681 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg
     4741 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc
     4801 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagcgaa
     4861 ttcgagctct aggaggcaaa aatgagcaat gattaccctt tagtatccat aataataccg
     4921 acgtataatt catctgatta cattactgaa actctaacga aattagaaaa acaaacttac
     4981 ccaaattttg aaattgttat tgttaatgat ggttctaaag ataacacatc aagtgttttg
     5041 agagagtatg ggttaaccca ctctcgatta attattatca ataaagaaaa tggcggtgtt
     5101 tcgtctgcca ggaatacagg tatccgcaag gcgcaaggac agtttatatg ttttatggat
     5161 gatgatgatg agatagatcc taactatctg ctgaagatgt attccagaca acatgagacg
     5221 ggaggggatg cgatttattg tgggctttat ggccatcata taaaaaatgg tgttacttac
     5281 tcacctataa atacagattt taatgaaggg tctttacttt tcgatttttt ttataaaaag
     5341 gtcagattcc atataggatg cttgtttata agaaaacaac tcctggaaga taataatctt
     5401 ttttttgatg aagatttacg actaggagaa gatctggatt ttatctatcg actgctaatt
     5461 acatgcgata tgtatgcggt tccatattat atgtataagc ataactatag agaaaattcc
     5521 ttaatgaact catgtagaac catcactcat tatcgacatg agtcatttgc gcacgaaaga
     5581 atctactctt ctgtgatgca gttatacaaa ggtaaccgga aagaagaaat tcatacatta
     5641 ttgagtaaaa atagaactta tcataaaact cgttatttat ggaatgttct acttaatggc
     5701 gattttgaat tattaaatca attagttgaa agcaatgaaa aagaattgaa agattgtaat
     5761 cttcttggca agagagataa gaggcgagca aagatattag catcaaaaaa ctatattctc
     5821 tggaggatgg taagactggt aaatagaaaa aagaataaac gttaggtctt gcattaaata
     5881 acccatcctc gtagcatagg ttgaaattat atgtcgtttt gttggaatga aattaactct
     5941 ggtgtcaagt ctttaattct catattatgt atgttgtctt taatgacttt gtctttgtgg
     6001 gatgatgttg caacaaagtt tcttcatgca gctggaatta tatctgcatt atattttcta
     6061 gcgacaccaa aaaaaacaat aactaataat cctactttgt taattttcat ctcattatgt
     6121 cttttgggta tcgttaatat catctggtat tcacattata aagtttcagg ctcggtttat
     6181 accaatgcat atcgtggccc aatggaaact ggaaaaattg ccttgtgtag tgcttttatt
     6241 ttcttagttc tttttgctaa agatgagatg agaaccaaaa taaaatttgg gaaactaatt
     6301 ctgttcgcat ccttggcaac gcagttactt ttttttgcgc atgccatgtg gcaacatttc
     6361 tatttaaacg tcgaccgtgt tgcattatca gcttcccacg ctacaacagc aggctacatc
     6421 atccttttcc cttctttact ggcatcaatt ctcattttaa aatccgactt tagacataaa
     6481 acaacattat atacaattaa cttcatgctt agcttatgtg ctgttatagt aactgaaacg
     6541 cgtgcagcca tattagtgtt tccattcttt gcgttactat taatcgtaat ggatagttat
     6601 attaataagc gaattaatta taagttatat tgttttattg cgattgcatt attagcaggt
     6661 gtattttctt ttaaagatac attacttacg agaatgaatg acttaaatag ggatttagtt
     6721 aattattcgc atgataacac cagaacttca gtcggtgccc gtctggcaat gtatgaagtt
     6781 ggtttaaaaa catattctcc aataggacaa tcactggaaa aacgtgcgga aaaaatacat
     6841 gaactagaag agaaagagcc tagattaagt ggtgctttac cctttgtaga ttctcatttg
     6901 cataacgatc tcatagatac gttatcaacg cgtggtattc caggagtagc attaacaatt
     6961 ttagcctttt cagcaatatt catatatgcc ttaagaactg ctaaagaacc ttatatttta
     7021 atcttgcttt tttcactact ggtagtaggc ctaagtgatg taatactctt ttctaaaccg
     7081 gttccgactg ctgtgtttgt caccataata ttgctttgtg cttattttaa agtacaatcg
     7141 gaccaatgtt tattagataa gtaagcatgc aagcttggct gttttg
//
