LOCUS       sequence-416581-       12416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of HB-EGF, the plasmid encodes a recombinant 
            HB-EGF fused extracellularly to the mScarlet fluorescent protein and
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pCSIIpuro-HBEGF-ScNeo
FEATURES             Location/Qualifiers
     source          1..12416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4030..4725
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4163..4181)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4431..4450
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5925..6474
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6069..6086)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6296..6315
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6477..7076
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6477..6496)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6973..6993
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7111..7699
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7164..7184)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7582..7593)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8218..8398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8424..8441)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8430..8654
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8700..9128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8787..8806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8997..9018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9137..9157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9142..9471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9322..9457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9384..9403
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9519..9566
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9585..9959
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10089..10210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10126..10145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10180..10199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10259..10275)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10259..10275)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10272..10294)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10283..10299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10307..10313,10314..10331,10332..10337))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10352..10373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10490..10507)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10661..11249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10741..10760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11420..12211,12212..12280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12043..12062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12281..12385)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ccgaattcac catgaagctg
     3781 ctgccgtcgg tggtgctgaa gctctttctg gctgcagttc tctcggcact ggtgactggc
     3841 gagagcctgg agcggcttcg gagagggcta gctgctggaa ccagcaaccc ggaccctccc
     3901 actgtatcca cggaccagct gctaccccta ggaggcggcc gggaccggaa agtccgtgac
     3961 ttgcaagagg cagatctgga ccttttgaga gtcactttat cctccaagcc acaagcactc
     4021 gagggaggaa tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg
     4081 cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     4141 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     4201 tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcac caagcacccc
     4261 gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
     4321 atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct ggaggacggc
     4381 accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg
     4441 cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg
     4501 ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gaggccgcta cctggcggac
     4561 ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta caacgtcgac
     4621 cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca gtacgaacgc
     4681 tccgagggcc gccactccac cggcggcatg gacgagctgt acaagggagg ctcgagcgcc
     4741 acaccaaaca aggaggagca cgggaaaaga aagaagaaag gcaaggggct agggaagaag
     4801 agggacccat gtcttcggaa atacaaggac ttctgcatcc atggagaatg caaatatgtg
     4861 aaggagctcc gggctccctc ctgcatctgc cacccgggtt accatggaga gaggtgtcat
     4921 gggctgagcc tcccagtgga aaatcgctta tatacctatg accacacaac catcctggcc
     4981 gtggtggctg tggtgctgtc atctgtctgt ctgctggtca tcgtggggct tctcatgttt
     5041 aggtaccata ggagaggagg ttatgatgtg gaaaatgaag agaaagtgaa gttgggcatg
     5101 actaattccc acggcggatt cgaaatggta agcaaggggg aggaagataa tatggcttcc
     5161 cttccagcaa cgcacgaatt gcatatcttt ggaagtatta atggagttga tttcgacatg
     5221 gtggggcagg gtacgggaaa cccaaacgat ggctatgaag aacttaacct caagtctacc
     5281 aaaggcgatc tccaattctc accctggata ttggtcccac acatcggcta cgggttccat
     5341 caatacctgc cttatcctga cgggatgagt ccttttcaag ctgctatggt tgacggaagt
     5401 ggctaccaag ttcaccgcac aatgcaattt gaggatggtg catctcttac ggttaattac
     5461 cgatatacat atgagggttc acacatcaag ggggaagctc aggtcaaggg cactggattt
     5521 cccgcagatg gcccagttat gaccaattct ctgaccgcag ccgattggtg tcgaagcaag
     5581 aagacctacc ctaacgacaa gactatcatc tcaaccttca aatggtccta caccacaggt
     5641 aacggaaagc ggtataggtc tacagctcgg acaacatata cctttgcgaa gcctatggcc
     5701 gctaactatt tgaaaaacca accgatgtat gtttttcgga agaccgaatt gaaacattca
     5761 aaaacggagt tgaatttcaa agagtggcag aaggcattca ccgacgtaat gggtatggat
     5821 gagttgtata aataagcggc ggccgcggct ctagaggatc cgttaactaa cttaagctag
     5881 caacggtttc cctctagcgg gatcaattcc gccccccccc cctaacgtta ctggccgaag
     5941 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6001 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6061 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6121 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6181 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     6241 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6301 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6361 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6421 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatga
     6481 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6541 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6601 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6661 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6721 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6781 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6841 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6901 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6961 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     7021 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgactag
     7081 agttaacatc gagggatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     7141 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7201 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7261 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7321 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7381 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7441 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7501 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7561 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7621 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7681 ctttgggccg cctccccgca tcgataccgt cgagacctag aaaaacatgg agcaatcaca
     7741 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     7801 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     7861 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7921 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     7981 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     8041 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     8101 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     8161 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     8221 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8281 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8341 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     8401 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     8461 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     8521 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     8581 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8641 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     8701 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8761 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8821 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8881 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8941 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     9001 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9061 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9121 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     9181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     9241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     9301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     9421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     9481 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     9541 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     9601 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     9661 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     9721 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     9781 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     9841 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     9901 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     9961 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
    10021 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    10081 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10141 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10201 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    10261 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    10321 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    10381 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    10441 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10501 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10561 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10621 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10681 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10741 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10801 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10861 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10921 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10981 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    11041 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    11101 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    11161 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    11221 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    11281 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    11341 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    11401 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    11461 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    11521 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    11581 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11641 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11701 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    11761 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11821 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11881 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11941 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    12001 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    12061 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    12121 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    12181 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    12241 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    12301 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    12361 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
