LOCUS       sequence-416598-       12344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of Epiregulin, the plasmid encodes a 
            recombinant Epiregulin fused extracellularly to the mScarlet 
            fluorescent protein and intracellularly to the mNeonGreen 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pCSIIpuro-EREG-ScNeo
FEATURES             Location/Qualifiers
     source          1..12344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3973..3999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4000..4695
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4133..4151)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4401..4420
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5853..6402
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5997..6014)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6224..6243
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6405..7004
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6405..6424)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6901..6921
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7039..7627
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7092..7112)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7510..7521)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8146..8326
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8352..8369)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8358..8582
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8628..9056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8715..8734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8925..8946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9065..9085)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9070..9399
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9250..9385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9312..9331
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9447..9494
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9513..9887
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10017..10138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10054..10073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10108..10127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10187..10203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10187..10203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10200..10222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10211..10227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10235..10241,10242..10259,10260..10265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10280..10301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10418..10435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10589..11177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10669..10688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11348..12139,12140..12208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11971..11990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12209..12313)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ccgacgaatt ctccaccatg
     3781 accgcgggga ggaggatgga gatgctctgt gccggcaggg tccctgcgct gctgctctgc
     3841 ctgggtttcc atcttctaca ggcagtcctc agtacaactg tgattccatc atgtatccca
     3901 ggagagtcca gtgataactg cacagcttta gttcagacag aagacaatcc acgtgtggct
     3961 caagtgtcaa tatacccata cgatgttccg gattacgcta tggtgagcaa gggcgaggca
     4021 gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag
     4081 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4141 aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg
     4201 tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta taagcagtcc
     4261 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc
     4321 gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc
     4381 accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc
     4441 accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat ggccctgcgc
     4501 ctgaaggacg gaggccgcta cctggcggac ttcaagacca cctacaaggc caagaagccc
     4561 gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc ccacaacgag
     4621 gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac cggcggcatg
     4681 gacgagctgt acaagggtgg tggtagtggt ggtggttcag tgtcaataac aaagtgtagc
     4741 tctgacatga atggctattg tttgcatgga cagtgcatct atctggtgga catgagtcaa
     4801 aactactgca ggtgtgaagt gggttatact ggtgtccgat gtgaacactt ctttttaacc
     4861 gtccaccaac ctttaagcaa agaatatgtg gctttgaccg tgattcttat tattttgttt
     4921 cttatcacag tcgtcggttc cacatattat ttctgcagat ggtacagaaa tcgaaaaagt
     4981 aaagaaccaa agaaggaata tgagagagtt acctcagggg atccagagtt gccgcaagtc
     5041 ggcggattcg aaatggtaag caagggggag gaagataata tggcttccct tccagcaacg
     5101 cacgaattgc atatctttgg aagtattaat ggagttgatt tcgacatggt ggggcagggt
     5161 acgggaaacc caaacgatgg ctatgaagaa cttaacctca agtctaccaa aggcgatctc
     5221 caattctcac cctggatatt ggtcccacac atcggctacg ggttccatca atacctgcct
     5281 tatcctgacg ggatgagtcc ttttcaagct gctatggttg acggaagtgg ctaccaagtt
     5341 caccgcacaa tgcaatttga ggatggtgca tctcttacgg ttaattaccg atatacatat
     5401 gagggttcac acatcaaggg ggaagctcag gtcaagggca ctggatttcc cgcagatggc
     5461 ccagttatga ccaattctct gaccgcagcc gattggtgtc gaagcaagaa gacctaccct
     5521 aacgacaaga ctatcatctc aaccttcaaa tggtcctaca ccacaggtaa cggaaagcgg
     5581 tataggtcta cagctcggac aacatatacc tttgcgaagc ctatggccgc taactatttg
     5641 aaaaaccaac cgatgtatgt ttttcggaag accgaattga aacattcaaa aacggagttg
     5701 aatttcaaag agtggcagaa ggcattcacc gacgtaatgg gtatggatga gttgtataaa
     5761 taagcggcgg ccgcggctct agaggatccg ttaactaact taagctagca acggtttccc
     5821 tctagcggga tcaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata
     5881 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     5941 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     6001 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     6061 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     6121 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     6181 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6241 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6301 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     6361 gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatgacc gagtacaagc
     6421 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     6481 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     6541 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     6601 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6661 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6721 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6781 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6841 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6901 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6961 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactagag ttaacatcga
     7021 gggatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7081 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7141 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7201 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7261 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7321 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7381 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     7441 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7501 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7561 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7621 tccccgcatc gataccgtcg agacctagaa aaacatggag caatcacaag tagcaataca
     7681 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     7741 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7801 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7861 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     7921 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     7981 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     8041 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     8101 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     8161 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8221 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8281 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     8341 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8401 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     8461 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     8521 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     8581 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     8641 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8701 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8761 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8821 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8881 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8941 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9001 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     9061 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     9121 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     9241 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9301 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     9361 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     9421 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     9481 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     9541 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     9601 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     9661 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     9721 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     9781 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     9841 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     9901 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     9961 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    10021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    10081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10141 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    10201 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    10261 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    10321 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    10381 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    10441 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    10501 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10561 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10621 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10681 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10741 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10801 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10861 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10921 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10981 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    11041 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    11101 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    11161 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    11221 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    11281 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    11341 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    11401 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    11461 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    11521 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    11581 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    11641 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    11701 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11761 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11821 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    11881 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    11941 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    12001 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    12061 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    12121 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    12181 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    12241 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    12301 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
