LOCUS       sequence-416612-       12260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of Epigen, the plasmid encodes a recombinant 
            Epigen fused extracellularly to the mScarlet fluorescent protein and
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12260)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 12260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pCSIIpuro-EPGN-ScNeo
FEATURES             Location/Qualifiers
     source          1..12260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3946..4641
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4079..4097)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4347..4366
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5769..6318
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5913..5930)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6140..6159
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6321..6920
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6321..6340)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6817..6837
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6955..7543
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7008..7028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7426..7437)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8062..8242
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8268..8285)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8274..8498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8544..8972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8631..8650)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8841..8862
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8981..9001)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8986..9315
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9166..9301
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9228..9247
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9363..9410
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9429..9803
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9933..10054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9970..9989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10024..10043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10103..10119)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10103..10119)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10116..10138)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10127..10143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10151..10157,10158..10175,10176..10181))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10196..10217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10334..10351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10505..11093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10585..10604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11264..12055,12056..12124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11887..11906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12125..12229)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ccgaattctc caccatggct
     3781 ttgggagttc caatatcagt ctatctttta ttcaacgcaa tgacagcact gaccgaagag
     3841 gcagccgtga ctgtaacacc tccaatcaca gcccagcaag gtaactggac agttaacaaa
     3901 acagaagctg acaacataga aggacccata gccctcgagg gaggaatggt gagcaagggc
     3961 gaggcagtga tcaaggagtt catgcggttc aaggtgcaca tggagggctc catgaacggc
     4021 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     4081 aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct gtcccctcag
     4141 ttcatgtacg gctccagggc cttcaccaag caccccgccg acatccccga ctactataag
     4201 cagtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgcc
     4261 gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa ggtgaagctc
     4321 cgcggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat gggctgggaa
     4381 gcgtccaccg agcggttgta ccccgaggac ggcgtgctga agggcgacat taagatggcc
     4441 ctgcgcctga aggacggagg ccgctacctg gcggacttca agaccaccta caaggccaag
     4501 aagcccgtgc agatgcccgg cgcctacaac gtcgaccgca agttggacat cacctcccac
     4561 aacgaggact acaccgtggt ggaacagtac gaacgctccg agggccgcca ctccaccggc
     4621 ggcatggacg agctgtacaa gggaggctcg agcttgaagt tctcacacct ttgcctggaa
     4681 gatcataaca gttactgcat caacggtgct tgtgcattcc accatgagct agagaaagcc
     4741 atctgcaggt gttttactgg ttatactgga gaaaggtgtg agcacttgac tttaacttca
     4801 tatgctgtgg attcttatga aaaatacatt gcaattggga ttggtgttgg attactatta
     4861 agtggttttc ttgttatttt ttactgctat ataagaaaga ggtgtctaaa attgaaatcg
     4921 ccttacaatg tctgttctgg agaaagacga ccactgggcg gattcgaaat ggtaagcaag
     4981 ggggaggaag ataatatggc ttcccttcca gcaacgcacg aattgcatat ctttggaagt
     5041 attaatggag ttgatttcga catggtgggg cagggtacgg gaaacccaaa cgatggctat
     5101 gaagaactta acctcaagtc taccaaaggc gatctccaat tctcaccctg gatattggtc
     5161 ccacacatcg gctacgggtt ccatcaatac ctgccttatc ctgacgggat gagtcctttt
     5221 caagctgcta tggttgacgg aagtggctac caagttcacc gcacaatgca atttgaggat
     5281 ggtgcatctc ttacggttaa ttaccgatat acatatgagg gttcacacat caagggggaa
     5341 gctcaggtca agggcactgg atttcccgca gatggcccag ttatgaccaa ttctctgacc
     5401 gcagccgatt ggtgtcgaag caagaagacc taccctaacg acaagactat catctcaacc
     5461 ttcaaatggt cctacaccac aggtaacgga aagcggtata ggtctacagc tcggacaaca
     5521 tatacctttg cgaagcctat ggccgctaac tatttgaaaa accaaccgat gtatgttttt
     5581 cggaagaccg aattgaaaca ttcaaaaacg gagttgaatt tcaaagagtg gcagaaggca
     5641 ttcaccgacg taatgggtat ggatgagttg tataaataag cggcggccgc ggctctagag
     5701 gatccgttaa ctaacttaag ctagcaacgg tttccctcta gcgggatcaa ttccgccccc
     5761 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     5821 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     5881 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     5941 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     6001 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     6061 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     6121 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     6181 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     6241 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     6301 ttgaaaaaca cgataatacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     6361 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6421 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6481 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     6541 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     6601 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6661 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     6721 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6781 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6841 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6901 cccgcaagcc cggtgcctga ctagagttaa catcgaggga tcaagcttat cgataatcaa
     6961 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7021 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7081 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7141 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7201 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7261 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7321 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     7381 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7441 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7501 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgagac
     7561 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7621 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7681 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7741 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7801 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     7861 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     7921 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7981 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     8041 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8221 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     8281 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     8341 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     8401 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     8461 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     8521 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8581 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8641 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8701 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8761 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8821 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8881 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     8941 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     9001 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9061 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9121 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9181 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9241 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9301 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     9361 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     9421 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     9481 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     9541 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     9601 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     9661 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     9721 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     9781 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     9841 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     9901 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     9961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10021 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    10081 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10141 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10201 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10261 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10321 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    10381 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    10441 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10501 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10561 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10621 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10681 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10741 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10801 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    10861 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    10921 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    10981 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11041 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11101 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11161 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11221 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11281 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    11341 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    11401 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    11461 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11521 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    11581 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11641 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11701 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11761 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    11821 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    11881 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    11941 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    12001 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    12061 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12121 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    12181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    12241 gaaaagtgcc acctgacgtc
//
