LOCUS       sequence-416614-       11007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Shafraz O, Davis CMO, Sivasankar S
  TITLE     Light-activated BioID - an optically activated proximity labeling 
            system to study protein-protein interactions.
  JOURNAL   J Cell Sci. 2023 Oct 1;136(19):jcs261430. doi: 10.1242/jcs.261430. 
            Epub 2023 Oct 11.
  PUBMED    37756605
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2023-10-1"; volume: "136(19):jcs261430. doi"; pages: "
            10.1242/jcs.261430. Epub 2023 Oct 11"
COMMENT     Sequence Label: BSD-CryC
FEATURES             Location/Qualifiers
     source          1..11007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(7..1626,1627..1632,1633..1842)
                     /codon_start=1
                     /product="Arabidopsis thaliana cryptochrome 2 in which the 
                     nuclear localization signal was removed by mutating 
                     residues 541 and 542 to alanine"
                     /label=CRY2(Delta-NLS)
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKE
                     PGLCPSVSSNDQQVPSAVRYNGSAAVKPEEEEERDMKKSRGFDERELFSTAESSSSSSV
                     FFVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK"
     regulatory      2647..2656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2653..3363
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2798..2816)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3066..3085
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3396..3984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3449..3469)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3867..3878)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4056..4289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4367..4488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4404..4423)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4458..4477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4528..4663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4683..4702)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4684..4702)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4712..4729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4712..4728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4721..4743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4870..5325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4957..4976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5167..5188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5351..5455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5456..5524,5525..6316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6487..7075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6976..6995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7229..7246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7363..7384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7399..7404,7405..7422,7423..7429)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7437..7453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7442..7464
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7461..7477
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7461..7477
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7496..7516
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7498..7516
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7544..7770
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7771..7951
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7998..8123
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8616..8849
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9034..9078
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9253..9753
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9672..9690
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9765..10160
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10220..10337
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10454..10757
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10758..10961
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10912..10932
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    join(10984..11007,1)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgaaatga agatggacaa aaagactata gtttggttta gaagagacct aaggattgag
       61 gataatcctg cattagcagc agctgctcac gaaggatctg tttttcctgt cttcatttgg
      121 tgtcctgaag aagaaggaca gttttatcct ggaagagctt caagatggtg gatgaaacaa
      181 tcacttgctc acttatctca atccttgaag gctcttggat ctgacctcac tttaatcaaa
      241 acccacaaca cgatttcagc gatcttggat tgtatccgcg ttaccggtgc tacaaaagtc
      301 gtctttaacc acctctatga tcctgtttcg ttagttcggg accataccgt aaaggagaag
      361 ctggtggaac gtgggatctc tgtgcaaagc tacaatggag atctattgta tgaaccgtgg
      421 gagatatact gcgaaaaggg caaacctttt acgagtttca attcttactg gaagaaatgc
      481 ttagatatgt cgattgaatc cgttatgctt cctcctcctt ggcggttgat gccaataact
      541 gcagcggctg aagcgatttg ggcgtgttcg attgaagaac tagggctgga gaatgaggcc
      601 gagaaaccga gcaatgcgtt gttaactaga gcttggtctc caggatggag caatgctgat
      661 aagttactaa atgagttcat cgagaagcag ttgatagatt atgcaaagaa cagcaagaaa
      721 gttgttggga attctacttc actactttct ccgtatctcc atttcgggga aataagcgtc
      781 agacacgttt tccagtgtgc ccggatgaaa caaattatat gggcaagaga taagaacagt
      841 gaaggagaag aaagtgcaga tctttttctt aggggaatcg gtttaagaga gtattctcgg
      901 tatatatgtt tcaacttccc gtttactcac gagcaatcgt tgttgagtca tcttcggttt
      961 ttcccttggg atgctgatgt tgataagttc aaggcctgga gacaaggcag gaccggttat
     1021 ccgttggtgg atgccggaat gagagagctt tgggctaccg gatggatgca taacagaata
     1081 agagtgattg tttcaagctt tgctgtgaag tttcttctcc ttccatggaa atggggaatg
     1141 aagtatttct gggatacact tttggatgct gatttggaat gtgacatcct tggctggcag
     1201 tatatctctg ggagtatccc cgatggccac gagcttgatc gcttggacaa tcccgcgtta
     1261 caaggcgcca aatatgaccc agaaggtgag tacataaggc aatggcttcc cgagcttgcg
     1321 agattgccaa ctgaatggat ccatcatcca tgggacgctc ctttaaccgt actcaaagct
     1381 tctggtgtgg aactcggaac aaactatgcg aaacccattg tagacatcga cacagctcgt
     1441 gagctactag ctaaagctat ttcaagaacc cgtgaagcac agatcatgat cggagcagca
     1501 cctgatgaga ttgtagcaga tagcttcgag gccttagggg ctaataccat taaagaacct
     1561 ggtctttgcc catctgtgtc ttctaatgac caacaagtac cttcggctgt tcgttacaac
     1621 gggtcagcgg cagtgaaacc tgaggaagaa gaagagagag acatgaagaa atctagggga
     1681 ttcgatgaaa gggagttgtt ttcgactgct gaatcttctt cttcttcgag tgtgtttttc
     1741 gtttcgcagt cttgctcgtt ggcatcagaa gggaagaatc tggaaggtat tcaagattca
     1801 tctgatcaga ttactacaag tttgggaaaa aatggttgca aagcccgggg taagggctcg
     1861 ggctcgacct cgggctcggg cggacagctg gacggcggga gcgtggcagt cctgcctgtg
     1921 gtcgactcca ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct
     1981 tgcattgcag aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct
     2041 tttggagcta acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc
     2101 ggcctgggcc cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac
     2161 aaggtgcgag tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc
     2221 ctggtggagc tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt
     2281 aacgtggcta tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag
     2341 gaagcaggga ttaacctgga caggaatact ctggccgcta cgctgatccg agagctgcgg
     2401 gcagccctgg aactgttcga gcaggaaggc ctggctccat atctgccacg gtgggagaag
     2461 ctggataact tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg
     2521 attagccggg ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa
     2581 ccctggatgg gcggagaaat cagtctgcgg tctgccgaaa agaagggctc gggcgatcca
     2641 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     2701 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     2761 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2821 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     2881 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     2941 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3001 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3061 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3121 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     3181 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     3241 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     3301 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3361 taggctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat tacaaaattt
     3421 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3481 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3541 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3601 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3661 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3721 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3781 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3841 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3901 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3961 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     4021 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     4081 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     4141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4261 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     4321 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     4381 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4441 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     4501 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4561 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4621 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     4681 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     4741 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4801 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4861 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4921 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4981 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5041 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5101 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5161 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5221 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5281 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     5341 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5401 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5461 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5521 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5581 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5641 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5701 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5761 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5821 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5881 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5941 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6001 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6061 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6121 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6181 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6241 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6301 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6361 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6421 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6481 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6541 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6601 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6661 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6721 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6781 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6841 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6901 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6961 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7021 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7081 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7141 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7201 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7261 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     7321 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7381 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7441 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     7501 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7561 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7621 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7681 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7741 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     7801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7921 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7981 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8041 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8101 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8161 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8221 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8281 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8341 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8401 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8461 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8521 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8581 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8641 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8701 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8761 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8821 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8881 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8941 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     9001 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9061 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9121 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9181 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9241 ccgaggggac ccttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     9301 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     9361 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     9421 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     9481 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     9541 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     9601 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     9661 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     9721 ctcgttgacc gaatcaccga cctctctccc cagggggtac caccatggcc aagcctttgt
     9781 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     9841 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     9901 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     9961 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
    10021 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
    10081 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
    10141 ctggttatgt gtgggagggc ctgcagctgc agtagtaaga attctagatc ttgagacaaa
    10201 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    10261 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    10321 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
    10381 tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt catagcccat
    10441 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
    10501 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
    10561 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
    10621 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
    10681 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
    10741 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
    10801 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
    10861 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
    10921 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actgtcggga
    10981 tcaacaagtt tgtacaaaaa agcaggc
//
