LOCUS       sequence-416622-       12215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and
            expression of the sgRNA from the kasOP* promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12215)
  AUTHORS   Maleckis M, Wibowo M, Gren T, Jarmusch SA, Sterndorff EB, Booth T, 
            Henriksen NNSE, Whitford CM, Jiang X, Jorgensen TS, Ding L, Weber T
  TITLE     Biosynthesis of the Azoxy Compound Azodyrecin from Streptomyces 
            mirabilis P8-A2.
  JOURNAL   ACS Chem Biol. 2024 Feb 10. doi: 10.1021/acschembio.3c00632.
  PUBMED    38340355
REFERENCE   2  (bases 1 to 12215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2024 Feb 10. doi: 10.1021/acschembio.3c00632."
COMMENT     Sequence Label: pCRISPR-cBEST-v2-kasOP*
FEATURES             Location/Qualifiers
     source          1..12215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            466..575
                     /note="incP origin of transfer"
     CDS             891..1691
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDSFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELLAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      1993..2580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2481..2500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2734..2751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2868..2889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2904..2909,2910..2927,2928..2934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2942..2958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2947..2969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2966..2982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2966..2982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        3087..3162
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(3332..3366)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             3534..4220
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(4269..4292,4293..4295,4296..8369)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8382..8633
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     terminator      complement(8814..8862)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             10577..12019
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgacccgt gccgggccgc agcggatcgc ggcagcagga gctccccgcc
      181 cgccacggct ggctggatgg tcgcctcacg cgcccgcagg ggtgacggcg tgggtgcggc
      241 ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga ccgcgaccac ccctcccttc
      301 ccgcctgccg tctttcaccg cccggccggt ccgcagacgc cgtacggcca ggccacggag
      361 gattacgggc agatggcgcc cacccggcac ccgggctggg gcaccctgga cagcacgaag
      421 gtccgcccca ctcaccgggg cgggccttcg cgttgagcta gagttcccgc cagcctcgca
      481 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
      541 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
      601 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
      661 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaat gcagctcacg
      721 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
      781 atgccggcct ttgaatgggt tcatgtgcag ctccataagc aaaaggggat gataagttta
      841 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
      901 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
      961 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     1021 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctgcg ctgggtccgg
     1081 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     1141 cgtcgcccgt tacaccggac cttggagttg tctctgactc attctggcgc ctgccaaatg
     1201 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     1261 tctctgatcc attgcccctg ccacctcact cgcctgcaag cccggtcgcc cgtgtccatg
     1321 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     1381 ttgccgagtt gttggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     1441 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     1501 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     1561 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     1621 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     1681 agtcgattgg ctgagctcat aagttcctat tccgaagttc ctattcgaaa tgaccgacca
     1741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1861 gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat
     1921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt cgtgcacaca gcccagcttg
     2341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2821 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     2881 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     2941 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaattgtaca
     3001 agctttgttc acattcgaac cgtctctgct ttgacaacat gctgtgcggt gttgtaaagt
     3061 cgtggccagc tagcgtcaga tccatggttt tagagctaga aatagcaagt taaaataagg
     3121 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttta cgtaggcgtc
     3181 agctacgatg ttccggggac tgctgatccg gtcagcaggt ggaagaggga ctggattcca
     3241 aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg ttgacgacga catggctcga
     3301 ttggcgcgac aagttgctgc gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     3361 tctgaacaaa tccagatgga gttctgaggt cattactgga ccggatcggg gatctgggct
     3421 gagggagccg acggcacgcg gcggctcacg gcgtggcacg cggaacgtcc gggcttgcac
     3481 ctcacgtcac gtgaggaggc agcgtggacg gcgtcagaga agggagcgga catatgtcct
     3541 ccgagaccgg ccccgtggcc gtggacccga ccctgcgtcg ccgcatcgag ccgcacgagt
     3601 tcgaggtctt cttcgacccg cgcgagctgc gcaaggagac ctgcctgctg tacgagatca
     3661 actggggcgg ccggcactcc atctggcgcc acacctcgca gaacaccaac aagcacgtcg
     3721 aggtcaactt catcgagaag ttcaccaccg agcggtactt ctgcccgaac acccgctgct
     3781 ccatcacctg gttcctgtcc tggtccccct gcggcgagtg ctcccgggcc atcaccgagt
     3841 tcctgtcccg ctacccgcac gtcaccctgt tcatctacat cgcccggctg taccaccacg
     3901 ccgacccgcg caaccggcag ggcctgcgcg acctgatctc ctccggcgtc accatccaga
     3961 tcatgaccga gcaggagtcc ggctactgct ggcggaactt cgtcaactac tccccgtcca
     4021 acgaggccca ctggccccgc tacccgcacc tgtgggtccg cctgtacgtc ctggagctgt
     4081 actgcatcat cctgggcctg ccgccctgcc tgaacatcct gcgtcgcaag cagccgcagc
     4141 tgaccttctt caccatcgcc ctccagtcct gccactacca gcgcctgccg ccccacatcc
     4201 tgtgggccac cggcctgaag tccggctccg agaccccggg cacctcggag tccgccaccc
     4261 cggagtccga caagaagtac tccatcggcc tcgcgatcgg caccaactcc gtgggctggg
     4321 cggtcatcac cgacgagtac aaggtcccct ccaagaagtt caaggtcctg ggcaacaccg
     4381 accggcactc gatcaagaag aacctgatcg gcgccctgct cttcgacagc ggcgagaccg
     4441 ccgaggcgac ccgcctgaag cggaccgcgc gtcgccgcta cacccggcgc aagaaccgca
     4501 tctgctacct gcaggaaatc ttctccaacg agatggccaa ggtggacgac tcgttcttcc
     4561 accgcctgga ggagagcttc ctggtggagg aggacaagaa gcacgagcgc cacccgatct
     4621 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctcc
     4681 gcaagaagct ggtggactcg accgacaagg cggacctgcg gctcatctac ctggccctcg
     4741 cgcacatgat caagttccgc ggccacttcc tcatcgaggg cgacctgaac ccggacaact
     4801 ccgacgtgga caagctcttc atccagctgg tgcagaccta caaccagctg ttcgaggaga
     4861 accccatcaa cgccagcggc gtggacgcca aggcgatcct ctccgcgcgc ctgagcaagt
     4921 cccggcgcct ggagaacctc atcgcccagc tgccgggcga gaagaagaac ggcctcttcg
     4981 gcaacctgat cgcgctgtcg ctcggcctga cccccaactt caagagcaac ttcgacctgg
     5041 ccgaggacgc gaagctccag ctgtccaagg acacctacga cgacgacctg gacaacctgc
     5101 tcgcccagat cggcgaccag tacgcggacc tcttcctggc cgcgaagaac ctctcggacg
     5161 ccatcctgct cagcgacatc ctgcgggtca acaccgagat caccaaggcc ccgctgtcgg
     5221 cgagcatgat caagcggtac gacgagcacc accaggacct gaccctgctc aaggccctcg
     5281 tgcgccagca gctgcccgag aagtacaagg aaatcttctt cgaccagtcc aagaacggct
     5341 acgccggcta catcgacggc ggcgcgtcgc aggaggagtt ctacaagttc atcaagccga
     5401 tcctggagaa gatggacggc accgaggagc tgctcgtcaa gctgaaccgc gaggacctgc
     5461 tccgcaagca gcggaccttc gacaacggct ccatcccgca ccagatccac ctgggcgagc
     5521 tccacgccat cctccggcgc caggaggact tctacccctt cctgaaggac aaccgcgaga
     5581 agatcgagaa gatcctgacc ttccgcatcc cgtactacgt cggccccctg gcccgcggca
     5641 actcccggtt cgcgtggatg acccggaagt cggaggagac catcaccccg tggaacttcg
     5701 aggaggtcgt ggacaagggc gcgtccgcgc agtcgttcat cgagcgcatg accaacttcg
     5761 acaagaacct cccgaacgag aaggtcctgc ccaagcactc cctgctctac gagtacttca
     5821 ccgtgtacaa cgagctgacc aaggtcaagt acgtgaccga gggcatgcgg aagccggcct
     5881 tcctgtcggg cgagcagaag aaggcgatcg tggacctgct cttcaagacc aaccgcaagg
     5941 tcaccgtgaa gcagctgaag gaggactact tcaagaagat cgagtgcttc gactccgtcg
     6001 agatcagcgg cgtggaggac cgcttcaacg cctccctggg cacctaccac gacctgctca
     6061 agatcatcaa ggacaaggac ttcctcgaca acgaggagaa cgaggacatc ctggaggaca
     6121 tcgtcctcac cctgaccctc ttcgaggacc gcgagatgat cgaggagcgg ctcaagacct
     6181 acgcccacct gttcgacgac aaggtgatga agcagctgaa gcgtcgccgc tacaccggct
     6241 ggggccgcct ctcccggaag ctgatcaacg gcatccggga caagcagagc ggcaagacca
     6301 tcctggactt cctcaagtcc gacggcttcg ccaaccgcaa cttcatgcag ctcatccacg
     6361 acgacagcct gaccttcaag gaggacatcc agaaggccca ggtctcgggc cagggcgaca
     6421 gcctccacga gcacatcgcc aacctggcgg gctccccggc gatcaagaag ggcatcctcc
     6481 agaccgtcaa ggtcgtggac gagctggtca aggtgatggg ccgccacaag cccgagaaca
     6541 tcgtgatcga gatggcccgg gagaaccaga ccacccagaa gggccagaag aactcgcgcg
     6601 agcggatgaa gcggatcgag gagggcatca aggagctcgg cagccagatc ctgaaggagc
     6661 acccggtcga gaacacccag ctgcagaacg agaagctgta cctctactac ctgcagaacg
     6721 gccgcgacat gtacgtggac caggagctcg acatcaaccg gctgtccgac tacgacgtgg
     6781 accacatcgt gccgcagtcc ttcctgaagg acgactcgat cgacaacaag gtcctgaccc
     6841 gctcggacaa gaaccggggc aagtccgaca acgtgccctc ggaggaggtc gtgaagaaga
     6901 tgaagaacta ctggcgccag ctgctcaacg ccaagctcat cacccagcgc aagttcgaca
     6961 acctgaccaa ggccgagcgg ggcggcctga gcgagctcga caaggcgggc ttcatcaagc
     7021 gccagctggt cgagacccgg cagatcacca agcacgtggc ccagatcctg gactcccgga
     7081 tgaacaccaa gtacgacgag aacgacaagc tgatccgcga ggtcaaggtg atcaccctca
     7141 agagcaagct ggtctccgac ttccgcaagg acttccagtt ctacaaggtc cgggagatca
     7201 acaactacca ccacgcccac gacgcgtacc tgaacgccgt cgtgggcacc gcgctgatca
     7261 agaagtaccc gaagctggag tccgagttcg tctacggcga ctacaaggtc tacgacgtgc
     7321 gcaagatgat cgccaagagc gagcaggaga tcggcaaggc caccgcgaag tacttcttct
     7381 actccaacat catgaacttc ttcaagaccg agatcaccct ggccaacggc gagatccgca
     7441 agcggcccct gatcgagacc aacggcgaga ccggcgagat cgtctgggac aagggccgcg
     7501 acttcgccac cgtccggaag gtgctgtcga tgccgcaggt caacatcgtg aagaagaccg
     7561 aggtgcagac cggcggcttc agcaaggagt ccatcctccc caagcgcaac agcgacaagc
     7621 tgatcgcccg gaagaaggac tgggacccga agaagtacgg cggcttcgac agccccaccg
     7681 tcgcctactc cgtgctggtc gtggcgaagg tcgagaaggg caagagcaag aagctgaagt
     7741 ccgtgaagga gctgctcggc atcaccatca tggagcgctc ctcgttcgag aagaacccga
     7801 tcgacttcct ggaggccaag ggctacaagg aggtcaagaa ggacctcatc atcaagctgc
     7861 ccaagtacag cctgttcgag ctggagaacg gccgcaagcg gatgctcgcc tccgcgggcg
     7921 agctgcagaa gggcaacgag ctggccctcc cgtcgaagta cgtcaacttc ctgtacctcg
     7981 cgtcccacta cgagaagctg aagggctcgc ccgaggacaa cgagcagaag cagctcttcg
     8041 tggagcagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttcagcaagc
     8101 gcgtcatcct ggccgacgcg aacctcgaca aggtgctgtc cgcctacaac aagcaccgcg
     8161 acaagccgat ccgggagcag gcggagaaca tcatccacct gttcaccctc accaacctgg
     8221 gcgcccccgc cgcgttcaag tacttcgaca ccaccatcga ccgcaagcgg tacacctcca
     8281 ccaaggaggt cctcgacgcg accctgatcc accagagcat caccggcctg tacgagaccc
     8341 gcatcgacct gtcccagctc ggcggcgact ccggcggctc caccaacctg tccgacatca
     8401 tcgagaagga gaccggcaag cagctggtca tccaggagtc catcctgatg ctgccggagg
     8461 aagtcgagga agtcatcggc aacaagcccg agtccgacat cctggtccac accgcctacg
     8521 acgagtccac cgacgagaac gtcatgctgc tgacctcgga cgcccccgag tacaagccct
     8581 gggccctggt catccaggac tccaacggcg agaacaagat caagatgctg tgatctagag
     8641 gatccccggg taccgagctc gaattcccca gatctaaagt tttgtcgtct ttccagacgt
     8701 tagtaaatga attttctgta tgaggttttg ctaaacaact ttcaacagtt tcagcggagt
     8761 gagaatagaa aggaacaact aaaggaattg cgaataataa ttttttcacg ttgaaaatct
     8821 ccaaaaaaaa aggctccaaa aggagccttt aattgtatcg gtttatcagc ttgctttcga
     8881 ggtgaatttc ttaaacagct tgataccgat agttgcgccg acaatgacaa caaccatcgc
     8941 ccacgcataa ccgatatatt cggtcgctga ggcttgcagg gagtcaaagg ccgcttttgc
     9001 gggatctcgt cgaaggcact agaggcctat atgcggggat cgaccgcgcg ggtcccggac
     9061 ggggaagagc ggggagcttt gccagagagc gacgacttcc ccttgcgttg gtgattgccg
     9121 gtcagggcag ccatccgcca tcgtcgcgta gggtgtcaca ccccaggaat cgcgtcactg
     9181 aacacagcag ccggtaggac gaccatgact gagttggaca ccatcgcaaa tccgtccgat
     9241 cccgcggtgc agcggatcat cgatgtcacc aagccgtcgc gatccaacat aaagacaacg
     9301 ttgatcgagg acgtcgagcc cctcatgcac agcatcgcgg ccggggtgga gttcatcgag
     9361 gtctacggca gcgacagcag tccttttcca tctgagttgc tggatctgtg cgggcggcag
     9421 aacataccgg tccgcctcat cgactcctcg atcgtcaacc agttgttcaa gggggagcgg
     9481 aaggccaaga cattcggcat cgcccgcgtc cctcgcccgg ccaggttcgg cgatatcgcg
     9541 agccggcgtg gggacgtcgt cgttctcgac ggggtgaaga tcgtcgggaa catcggcgcg
     9601 atagtacgca cgtcgctcgc gctcggagcg tcggggatca tcctggtcga cagtgacatc
     9661 accagcatcg cggaccggcg tctccaaagg gccagccgag gttacgtctt ctcccttccc
     9721 gtcgttctct ccggtcgcga ggaggccatc gccttcattc gggacagcgg tatgcagctg
     9781 atgacgctca aggcggatgg cgacatttcc gtgaaggaac tcggggacaa tccggatcgg
     9841 ctggccttgc tgttcggcag cgaaaagggt gggccttccg acctgttcga ggaggcgtct
     9901 tccgcctcgg tttccatccc catgatgagc cagaccgagt ctctcaacgt ttccgtttcc
     9961 ctcggaatcg cgctgcacga gaggatcgac aggaatctcg cggccaaccg ataagcgcct
    10021 ctgttcctcg gacgctcggt tgctcgacct cgattcgtca gtgatgatca acaggaccca
    10081 gaaatggcac gagcccggga ttggcctccc gggctcgccg cccccgaacc ggcgcagcat
    10141 gccgagtgag gacgtttgct ctgtgacgag cgtacgagag aacagcgcgt cgacaccacc
    10201 gcgccccgcc ggaccgaagt gccactgcgg caccccgatc gtccacatcc ccgggaagcg
    10261 ccccaaaatc tactgctcgg acgcctgccg aaagcgcgca aagcgtctca ttgcggacca
    10321 gtcacgcagc gtagcaagca cgactacagc gggtaatccg gacgcgggct ttagtagtaa
    10381 gggaaaatct ccgtccactg caggtcagag cgctgtaact ggaacgggtt ctaccccaaa
    10441 cgagggtgcc gcgacagccg ggcgggagga cccgcgcgac gagcggttcg cccggcggga
    10501 ccggcaccag acggtgtcgc tgaacgaggc gttcaagggc tgcgggaccc ggctcactgc
    10561 cgggacggcc gagctgatgt ggaagccggg cgaggcgacg tggggcaaca cgtgccgctg
    10621 caacaacgtc cacacctgcc cgtggtgcat gagccgcatc ctggccgtac ggggctcgaa
    10681 cgtgcagctc gccgccgacg gtctcgcgga cgccgggtac gggctgcacc ttggcacgaa
    10741 cacgttgcgc cacttcgagc gcatggcgtt cggcaccgtc cgcaagggaa tgcggcacgg
    10801 cctggtcgcg gtcttgcacg acggctggaa gggcgcgtac ggctcgtccg gccgccgctg
    10861 gcgcacgatg cgcgacgact tcgggatcat cggctacgag cgggccttcg aggacacctt
    10921 cggctggggc tccggctggc acctgcactg gcacacgctc tgggtcaccc gcgaggtgct
    10981 cggcccggac gcccaggccg cgttccgcga cgcactcgcc ggagcctggg ccgccggggt
    11041 cgaatccgcg ggcggctaca ccgtcagcga gacgtgcgac cggcccggtt gctcctgtga
    11101 gggcaagggc cacggcaccg acgtgcgccc actcaacggc gcggacgccg ctgacggcga
    11161 tgcgggcaag caggcccgct acctctacaa ggacggcgac aagaccaagg gcggcgtcgc
    11221 caagatcggc ctggagctgg ccggacagaa cttcaaggcc ggtcgcggcg acgaccgcat
    11281 gggaccgctc gacctgggcg acgcggcggc cgccgagctg cagcggcttc gtcggcccgg
    11341 cccgttcgtc gagaagtacc gcgagcgcga gttcggcgtc ttccaggtcc gcaagcacta
    11401 ccggtcgcag aacctgaacc ggctgatcaa ggaactcggc atccagcagg acgtccgcac
    11461 cgaggaggag atcaccgacg acaccgaggg cctggtcgcc atcgcggtca tcccggccta
    11521 catctggtac cgatacatcg cccgcgtcgc cggacgtcgc ctcgacctca tcaaggtcgc
    11581 cgagacgtac ggcctaccgg gcgtccggcg gctcgtcgag tcctggggcc tggtgtgggg
    11641 caaggacgtc ctggacccgc ccgccccgga agcgccggcc gcgccgggcg accttgacgc
    11701 tgaccagatg aggttcgagg tcatgtccga ggaggaagct gctttccgcg aggcgcgccg
    11761 gaaggccaac gaggcccgca cggaggagct ggccgcttcg ctcgaccgag tgcgtcagcc
    11821 gaagaaggag gcgatccgcc cgacgatcag ccttcggaag cgcctgaagc cgaagccggt
    11881 caccgtcgac gtcaagactc ccccgcccgg cgccgcgtcg cccgtgtgcc gccgctgcaa
    11941 gggcaagctc gccccggtac tccagccctg gggccagcac cccggagact gcctccgcgt
    12001 cgacacagcg gtcgcgtagg gccgctgagg gaccgccatg agaccgccac cccggacatc
    12061 cgggtggcgg tctcatggcg gtcccggcgc gaaagccgag ggtctgcctg ccgtgaggtg
    12121 gccggcggac ctgggttact gctggaatcc cgcgcccagt ccgagcgtcc gccggcgctt
    12181 ttcacctggc tccgggtgct cgtcgatgag cacca
//
