LOCUS       sequence-416639-        1886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PLBD2-6xHis S392A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Nyame K, Hims A, Aburous A, Laqtom NN, Dong W, Medoh UN, Heiby JC, 
            Xiong J, Ori A, Abu-Remaileh M
  TITLE     Glycerophosphodiesters inhibit lysosomal phospholipid catabolism in 
            Batten disease.
  JOURNAL   Mol Cell. 2024 Apr 4;84(7):1354-1364.e9. doi: 
            10.1016/j.molcel.2024.02.006. Epub 2024 Mar 5.
  PUBMED    38447580
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.02.006"; journalName: "Mol Cell"; date: 
            "2024-04-4- 4"; volume: "84"; issue: "7"; pages: "1354-1364.e9"
COMMENT     Sequence Label: pRK5-PLBD2-6xHis S392A
FEATURES             Location/Qualifiers
     source          1..1886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1826..1843
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcccaggtcc aactgcacct cggttctatc gattgaattc accatggtag gccagatgta
       61 ctgctatcca ggatctcatt tggctagagc tcttacaagg gctcttgcat tggctttagt
      121 cttagcattg ctcgtaggac cctttctctc cggtcttgca ggagcaatac ctgctccagg
      181 cggaaggtgg gcacgagatg ggcaggtccc tccagcctcc cgcagccgct cggtgctcct
      241 ggacgtctcg gcgggccagc tgcttatggt ggacggacgc caccctgacg ccgtggcctg
      301 ggccaacctc accaacgcca tccgcgagac tgggtgggcc ttcctggagc tgggcacaag
      361 tggccaatac aatgacagct tgcaggccta tgcagccggt gtggtggagg ctgctgtgtc
      421 ggaggagctc atctacatgc actggatgaa cacggtggtg aattactgcg gccccttcga
      481 gtatgaagtc ggctactgcg agaggctgaa gagcttcctg gaggccaacc tagagtggat
      541 gcaggaagag atggagtcaa acccagactc accttactgg caccaggtgc ggctgaccct
      601 cctgcagctg aaaggcctgg aggacagcta cgaaggccgt gtgagcttcc cagctgggaa
      661 gttcaccatc aaacccttgg ggttcctcct gctgcagctc tctggggacc tggaagacct
      721 ggagctggcc ctgaacaaga ccaagatcaa accttctctg ggctctggct cctgttctgc
      781 cctcatcaag ctgctccctg gccagagtga cctcctggtt gcccacaaca cctggaacaa
      841 ctaccagcac atgctgcgtg tcatcaagaa gtactggctc cagttccggg aaggcccctg
      901 gggggactac ccgctggttc ccggcaacaa gctggtcttc tcctcctacc ccggcaccat
      961 cttctcctgc gacgacttct acatcctggg cagtgggctg gtgacactgg agaccaccat
     1021 tggcaacaag aacccagccc tgtggaagta tgtgcggccc aggggctgtg tgctggagtg
     1081 ggtacgcaac atcgtggcca accgcctggc ctcggatggg gccacctggg cagacatctt
     1141 caagaggttc aacagcggca cgtataacaa ccagtggatg atcgtggact acaaggcgtt
     1201 catcccgggt gggcccgccc ccgggtcccg ggtgcttacc atcctggagc agatccccgg
     1261 catggtggtg gtggctgaca agacctcgga gctctaccag aagacctact gggccagcta
     1321 caacataccg tccttcgaga ctgtgttcaa tgccagtggg ctgcaggccc tagtggccca
     1381 gtatggggac tggttttctt atgacgggag cccccgggcc cagatcttcc ggcggaacca
     1441 gtcactggta caagacatgg actccatggt caggctgatg aggtacaatg acttcctcca
     1501 tgaccctctg tcactgtgca aagcctgcaa cccccagccc aatggggaga atgctatctc
     1561 cgcccgctcc gacctcaacc cggccaatgg ctcctacccc ttccaggccc tgcgtcagcg
     1621 ctcccatggg ggtatcgatg tgaaggtgac cagcatgtca ctggccagga tcctgagcct
     1681 gctggcggcc agcggtccca cgtgggacca ggtgcccccg ttccagtgga gcacctcgcc
     1741 cttcagcggc ctgctgcaca tgggccagcc agacctctgg aagttcgcgc ctgtcaaggt
     1801 ttcatgggac ggaggtggag gtagtcatca tcaccatcac cactaggcgg ccgctaagta
     1861 agtaaggatc cccagcttgg ccgcca
//
