LOCUS       sequence-416655-       12998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of Necl-5, the plasmid encodes a recombinant 
            Necl-5 fused extracellularly to the mScarlet fluorescent protein and
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 12998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pCSIIpuro-Necl5-ScNeo
FEATURES             Location/Qualifiers
     source          1..12998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1676..1909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2094..2138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2412..2529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2585..3747
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2816..3738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3695..3715
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3856..4551
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3989..4007)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4257..4276
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6507..7056
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6651..6668)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6878..6897
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7059..7658
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7059..7078)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7555..7575
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7693..8281
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7746..7766)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8164..8175)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8800..8980
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9006..9023)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9012..9236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9282..9710
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9369..9388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9579..9600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9719..9739)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9724..10053
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9904..10039
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9966..9985
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10101..10148
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10167..10541
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10671..10792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10708..10727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10762..10781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10841..10857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10841..10857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10854..10876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10865..10881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10889..10895,10896..10913,10914..10919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10934..10955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11072..11089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11243..11831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11323..11342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12002..12793,12794..12862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12625..12644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12863..12967)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gggccgctga tcttcagacc tggaggagga gatatgaggg
     1561 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1621 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1681 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1741 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1801 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1861 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1921 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1981 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2041 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2101 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2161 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2221 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2281 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2341 gtggagagag agacagagac agatccattc gattagtgaa cggatctaca aatggcagta
     2401 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2461 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2521 caaaattttc gggtttatta cagggacagc agaaattcac tttgaattaa ttcaagcttc
     2581 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2641 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2701 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2761 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2821 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2881 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
     2941 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     3001 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     3061 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     3121 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3181 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3241 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3301 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3361 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
     3421 ggagagcggg cgggtgagtc acccacacaa aggaaagggg cctttccgtc ctcagccgtc
     3481 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
     3541 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3601 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3661 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3721 ttttttcttc catttcaggt gtcgtgaaca cgctaccggt ccgaattcac catggctcaa
     3781 ctcgcccgag ccacccgctc cccgctgtca tggctgctgc tgctgttctg ctatgcactc
     3841 cggaaagcgg gtgggatggt gagcaagggc gaggcagtga tcaaggagtt catgcggttc
     3901 aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     3961 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     4021 cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc cttcaccaag
     4081 caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt caagtgggag
     4141 cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac ctccctggag
     4201 gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc tgacggcccc
     4261 gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta ccccgaggac
     4321 ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggagg ccgctacctg
     4381 gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg cgcctacaac
     4441 gtcgaccgca agttggacat cacctcccac aacgaggact acaccgtggt ggaacagtac
     4501 gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggaggctcg
     4561 gatatacgtg tgctggtgcc ctacaattcg acaggcgtct tgggagggtc gaccaccttg
     4621 cactgtagtc tgacttctaa tgagaatgtg actatcactc aaataacctg gatgaagaag
     4681 gattcaggtg gatcccacgc tcttgtggct gtcttccacc ccaagaaggg gcccaacatc
     4741 aaagagccag agagggtgaa attcttggct gcccaacagg atctgaggaa cgcatctctg
     4801 gccatctcga acttaagtgt agaagacgaa ggcatctatg aatgtcagat tgccacattc
     4861 cccagaggca gtagaagcac caatgcctgg ctgaaggtgc aagcccgacc taagaacact
     4921 gcagaggccc tggagccctc tcccaccttg atactgcagg atgtggctaa atgcatctct
     4981 gccaatggtc accctcctgg acgaatctct tggccctcga atgtgaatgg aagtcaccgt
     5041 gaaatgaagg aaccagggtc ccagccgggc accaccacag ttaccagcta cctctccatg
     5101 gtaccttctc gccaggcaga cggcaagaac atcacctgca cggtggagca tgaaagctta
     5161 caggagctgg accagctgct ggtgaccctt tcccaaccct atccacctga aaacgtgtcc
     5221 atctctggct atgacggcaa ctggtatgtt ggcctcacta acttgaccct gacctgtgaa
     5281 gctcacagca aaccagcgcc tgacatggct ggatataact ggagcacgaa cacgggtgac
     5341 tttcccaact ctgttaagcg ccagggcaat atgcttctaa tctccaccgt agaggatggt
     5401 ctcaataaca cggtcattgt gtgcgaagtc accaatgccc tagggtctgg gcagggccaa
     5461 gtgcacatca ttgttaaaga gaaacctgag aatatgcagc aaaatacaag attacaccta
     5521 ggctacatct ttcttatcgt ctttgtcctc gctgtagtca tcatcatcgc agcactatac
     5581 actatacgaa gatgcaggca tggtcgtgct ctgcagtcca atccctcaga gagggagaac
     5641 gtccagtatt catctgtgaa cggcgactgt agactgaaca tggagccaaa cagcacaagg
     5701 ttcgaaatgg taagcaaggg ggaggaagat aatatggctt cccttccagc aacgcacgaa
     5761 ttgcatatct ttggaagtat taatggagtt gatttcgaca tggtggggca gggtacggga
     5821 aacccaaacg atggctatga agaacttaac ctcaagtcta ccaaaggcga tctccaattc
     5881 tcaccctgga tattggtccc acacatcggc tacgggttcc atcaatacct gccttatcct
     5941 gacgggatga gtccttttca agctgctatg gttgacggaa gtggctacca agttcaccgc
     6001 acaatgcaat ttgaggatgg tgcatctctt acggttaatt accgatatac atatgagggt
     6061 tcacacatca agggggaagc tcaggtcaag ggcactggat ttcccgcaga tggcccagtt
     6121 atgaccaatt ctctgaccgc agccgattgg tgtcgaagca agaagaccta ccctaacgac
     6181 aagactatca tctcaacctt caaatggtcc tacaccacag gtaacggaaa gcggtatagg
     6241 tctacagctc ggacaacata tacctttgcg aagcctatgg ccgctaacta tttgaaaaac
     6301 caaccgatgt atgtttttcg gaagaccgaa ttgaaacatt caaaaacgga gttgaatttc
     6361 aaagagtggc agaaggcatt caccgacgta atgggtatgg atgagttgta taaataagcg
     6421 gcggccgcgg ctctagagga tccgttaact aacttaagct agcaacggtt tccctctagc
     6481 gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     6541 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     6601 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     6661 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     6721 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     6781 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     6841 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     6901 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     6961 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     7021 cggggacgtg gttttccttt gaaaaacacg ataataccat gaccgagtac aagcccacgg
     7081 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     7141 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     7201 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     7261 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     7321 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     7381 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     7441 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     7501 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     7561 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     7621 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgact agagttaaca tcgagggatc
     7681 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7741 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7801 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7861 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7921 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7981 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8041 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     8101 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8161 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8221 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8281 catcgatacc gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     8341 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     8401 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8461 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     8521 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     8581 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     8641 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     8701 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     8761 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     8821 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8881 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8941 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     9001 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     9061 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     9121 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     9181 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     9241 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     9301 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9361 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9421 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9481 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     9541 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     9601 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     9661 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     9721 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9781 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     9841 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9901 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9961 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
    10021 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
    10081 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
    10141 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
    10201 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
    10261 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
    10321 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
    10381 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
    10441 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
    10501 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
    10561 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    10621 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
    10681 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10741 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10801 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    10861 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    10921 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    10981 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    11041 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    11101 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    11161 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    11221 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    11281 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    11341 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    11401 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    11461 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    11521 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    11581 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    11641 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    11701 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    11761 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    11821 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    11881 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    11941 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    12001 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    12061 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    12121 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    12181 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    12241 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    12301 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    12361 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    12421 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    12481 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    12541 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    12601 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    12661 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    12721 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    12781 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    12841 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    12901 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    12961 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
