LOCUS       sequence-416690-        8860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of HB-EGF, the plasmid encodes a recombinant 
            HB-EGF fused extracellularly to the mScarlet fluorescent protein and
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pPBbsr2-HBEGF-ScNeo
FEATURES             Location/Qualifiers
     source          1..8860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1670
                     /label=chicken beta-actin promoter
     intron          1671..2687
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2611..2633
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2695..2714
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3015..3710
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3148..3166)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3416..3435
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4924..5473
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5068..5085)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5295..5314
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5944..5961)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5950..6174
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6221..6237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6245..6261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6269..6275,6276..6293,6294..6299))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6314..6335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6569..6631
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6672..6692)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6672..6690)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6711..6727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6735..6751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6759..6765,6766..6783,6784..6789))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6804..6825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6942..6959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7113..7701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7193..7212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7872..8663,8664..8732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8495..8514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8733..8837)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
     1561 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
     1621 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
     1681 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
     1741 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
     1801 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
     1861 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     1921 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     1981 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2041 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     2101 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
     2161 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
     2221 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     2281 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     2341 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     2401 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     2461 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     2521 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     2581 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     2641 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     2701 tgctggttat tgtgctgtct catcattttg gcaaagaatt gggatccgaa ttcaccatga
     2761 agctgctgcc gtcggtggtg ctgaagctct ttctggctgc agttctctcg gcactggtga
     2821 ctggcgagag cctggagcgg cttcggagag ggctagctgc tggaaccagc aacccggacc
     2881 ctcccactgt atccacggac cagctgctac ccctaggagg cggccgggac cggaaagtcc
     2941 gtgacttgca agaggcagat ctggaccttt tgagagtcac tttatcctcc aagccacaag
     3001 cactcgaggg aggaatggtg agcaagggcg aggcagtgat caaggagttc atgcggttca
     3061 aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     3121 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     3181 ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc ttcaccaagc
     3241 accccgccga catccccgac tactataagc agtccttccc cgagggcttc aagtgggagc
     3301 gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc tccctggagg
     3361 acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct gacggccccg
     3421 taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac cccgaggacg
     3481 gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggaggc cgctacctgg
     3541 cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc gcctacaacg
     3601 tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg gaacagtacg
     3661 aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggaggctcga
     3721 gcgccacacc aaacaaggag gagcacggga aaagaaagaa gaaaggcaag gggctaggga
     3781 agaagaggga cccatgtctt cggaaataca aggacttctg catccatgga gaatgcaaat
     3841 atgtgaagga gctccgggct ccctcctgca tctgccaccc gggttaccat ggagagaggt
     3901 gtcatgggct gagcctccca gtggaaaatc gcttatatac ctatgaccac acaaccatcc
     3961 tggccgtggt ggctgtggtg ctgtcatctg tctgtctgct ggtcatcgtg gggcttctca
     4021 tgtttaggta ccataggaga ggaggttatg atgtggaaaa tgaagagaaa gtgaagttgg
     4081 gcatgactaa ttcccacggc ggattcgaaa tggtaagcaa gggggaggaa gataatatgg
     4141 cttcccttcc agcaacgcac gaattgcata tctttggaag tattaatgga gttgatttcg
     4201 acatggtggg gcagggtacg ggaaacccaa acgatggcta tgaagaactt aacctcaagt
     4261 ctaccaaagg cgatctccaa ttctcaccct ggatattggt cccacacatc ggctacgggt
     4321 tccatcaata cctgccttat cctgacggga tgagtccttt tcaagctgct atggttgacg
     4381 gaagtggcta ccaagttcac cgcacaatgc aatttgagga tggtgcatct cttacggtta
     4441 attaccgata tacatatgag ggttcacaca tcaaggggga agctcaggtc aagggcactg
     4501 gatttcccgc agatggccca gttatgacca attctctgac cgcagccgat tggtgtcgaa
     4561 gcaagaagac ctaccctaac gacaagacta tcatctcaac cttcaaatgg tcctacacca
     4621 caggtaacgg aaagcggtat aggtctacag ctcggacaac atataccttt gcgaagccta
     4681 tggccgctaa ctatttgaaa aaccaaccga tgtatgtttt tcggaagacc gaattgaaac
     4741 attcaaaaac ggagttgaat ttcaaagagt ggcagaaggc attcaccgac gtaatgggta
     4801 tggatgagtt gtataaataa gcggcggccg ctctagagtc gacgggccgc ggtaacaatt
     4861 gttaactaac ttaagctagc aacggtttcc ctctagcggg atcaattccg cccccccccc
     4921 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     4981 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5041 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5101 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5161 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5221 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5281 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5341 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     5401 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5461 aaacacgata ataccatggt catgaaaaca tttaacattt ctcaacaaga tctagaatta
     5521 gtagaagtag cgacagagaa gattacaatg ctttatgagg ataataaaca tcatgtggga
     5581 gcggcaattc gtacgaaaac aggagaaatc atttcggcag tacatattga agcgtatata
     5641 ggacgagtaa ctgtttgtgc agaagccatt gcgattggta gtgcagtttc gaatggacaa
     5701 aaggattttg acacgattgt agctgttaga cacccttatt ctgacgaagt agatagaagt
     5761 attcgagtgg taagtccttg tggtatgtgt agggagttga tttcagacta tgcaccagat
     5821 tgttttgtgt taatagaaat gaatggcaag ttagtcaaaa ctacgattga agaactcatt
     5881 ccactcaaat atacccgaaa ttaaaagtcg agatgcatcg atgatctaga gctcgctgat
     5941 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6001 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6061 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6121 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6181 aggcggaaag aacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6241 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6301 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6361 tccagtcggg aaacctgtcg tgccagcgga tcttaaaagt tttgttactt tatagaagaa
     6421 attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat
     6481 ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat
     6541 aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa
     6601 cgtacgtcac aatatgatta tctttctagg gttaatctag tatacgcgtt taattaatcc
     6661 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     6721 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6901 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7081 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7741 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7861 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7921 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7981 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8041 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8101 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8161 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8221 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8281 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8341 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8401 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8461 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8521 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8581 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8641 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8701 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8761 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8821 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
