LOCUS       sequence-416691-        8752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of TGF-alpha, the plasmid encodes a 
            recombinant TGF-alpha, fused extracellularly to the mScarlet 
            fluorescent protein and intracellularly to the mNeonGreen 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pPBbsr2-TGFa-ScNeo
FEATURES             Location/Qualifiers
     source          1..8752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1680..2696
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2620..2642
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2704..2723
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2898..2924
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2925..3620
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3058..3076)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3326..3345
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4816..5365
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4960..4977)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5187..5206
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5836..5853)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5842..6066
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6113..6129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6137..6153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6161..6167,6168..6185,6186..6191))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6206..6227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6461..6523
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6564..6584)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6564..6582)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6603..6619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6627..6643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6651..6657,6658..6675,6676..6681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6696..6717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6834..6851)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7005..7593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7085..7104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7764..8555,8556..8624))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8387..8406
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8625..8729)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
     1561 ggggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     1621 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     1681 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     1741 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     1801 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     1861 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     1921 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     1981 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     2041 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     2101 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     2161 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     2221 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     2281 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     2341 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     2401 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     2461 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     2521 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     2581 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     2641 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     2701 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg ggatccgaat
     2761 tctccaccat ggtcccctcg gctggacagc tcgccctgtt cgctctgggt attgtgttgg
     2821 ctgcgtgcca ggccttggag aacagcacgt ccccgctgag tgcagacccg cccgtggctg
     2881 cagcagtggt gtcccattac ccatacgatg ttccggatta cgctatggtg agcaagggcg
     2941 aggcagtgat caaggagttc atgcggttca aggtgcacat ggagggctcc atgaacggcc
     3001 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     3061 agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg tcccctcagt
     3121 tcatgtacgg ctccagggcc ttcaccaagc accccgccga catccccgac tactataagc
     3181 agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg
     3241 tgaccgtgac ccaggacacc tccctggagg acggcaccct gatctacaag gtgaagctcc
     3301 gcggcaccaa cttccctcct gacggccccg taatgcagaa gaagacaatg ggctgggaag
     3361 cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt aagatggccc
     3421 tgcgcctgaa ggacggaggc cgctacctgg cggacttcaa gaccacctac aaggccaaga
     3481 agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa gttggacatc acctcccaca
     3541 acgaggacta caccgtggtg gaacagtacg aacgctccga gggccgccac tccaccggcg
     3601 gcatggacga gctgtacaag ggtggtggta gtggtggtgg ttcagtgtcc cattttaatg
     3661 actgcccaga ttcccacact cagttctgct tccatggaac ctgcaggttt ttggtgcagg
     3721 aggacaagcc agcatgtgtc tgccattctg ggtacgttgg tgcacgctgt gagcatgcgg
     3781 acctcctggc cgtggtggct gccagccaga agaagcaggc catcaccgcc ttggtggtgg
     3841 tctccatcgt ggccctggct gtccttatca tcacatgtgt gctgatacac tgctgccagg
     3901 tccgaaaaca ctgtgagtgg tgccgggccc tcatctgccg gcacgagaag cccagcgccc
     3961 tcctgaaggg aagaaccgct tgctgccact cagaaacaat ggtaagcaag ggggaggaag
     4021 ataatatggc ttcccttcca gcaacgcacg aattgcatat ctttggaagt attaatggag
     4081 ttgatttcga catggtgggg cagggtacgg gaaacccaaa cgatggctat gaagaactta
     4141 acctcaagtc taccaaaggc gatctccaat tctcaccctg gatattggtc ccacacatcg
     4201 gctacgggtt ccatcaatac ctgccttatc ctgacgggat gagtcctttt caagctgcta
     4261 tggttgacgg aagtggctac caagttcacc gcacaatgca atttgaggat ggtgcatctc
     4321 ttacggttaa ttaccgatat acatatgagg gttcacacat caagggggaa gctcaggtca
     4381 agggcactgg atttcccgca gatggcccag ttatgaccaa ttctctgacc gcagccgatt
     4441 ggtgtcgaag caagaagacc taccctaacg acaagactat catctcaacc ttcaaatggt
     4501 cctacaccac aggtaacgga aagcggtata ggtctacagc tcggacaaca tatacctttg
     4561 cgaagcctat ggccgctaac tatttgaaaa accaaccgat gtatgttttt cggaagaccg
     4621 aattgaaaca ttcaaaaacg gagttgaatt tcaaagagtg gcagaaggca ttcaccgacg
     4681 taatgggtat ggatgagttg tataaagtgg tctaagcggc cgctctagag tcgacgggcc
     4741 gcggtaacaa ttgttaacta acttaagcta gcaacggttt ccctctagcg ggatcaattc
     4801 cgcccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     4861 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     4921 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     4981 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5041 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5101 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5161 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5221 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5281 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5341 ttttcctttg aaaaacacga taataccatg gtcatgaaaa catttaacat ttctcaacaa
     5401 gatctagaat tagtagaagt agcgacagag aagattacaa tgctttatga ggataataaa
     5461 catcatgtgg gagcggcaat tcgtacgaaa acaggagaaa tcatttcggc agtacatatt
     5521 gaagcgtata taggacgagt aactgtttgt gcagaagcca ttgcgattgg tagtgcagtt
     5581 tcgaatggac aaaaggattt tgacacgatt gtagctgtta gacaccctta ttctgacgaa
     5641 gtagatagaa gtattcgagt ggtaagtcct tgtggtatgt gtagggagtt gatttcagac
     5701 tatgcaccag attgttttgt gttaatagaa atgaatggca agttagtcaa aactacgatt
     5761 gaagaactca ttccactcaa atatacccga aattaaaagt cgagatgcat cgatgatcta
     5821 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5881 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5941 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6001 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6061 ctatggcttc tgaggcggaa agaacctgca gcccaagctt ggcgtaatca tggtcatagc
     6121 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6181 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6241 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatcttaaaa gttttgttac
     6301 tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac ataaataaat
     6361 tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc
     6421 aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacgcat
     6481 gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaatct agtatacgcg
     6541 tttaattaat ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     6601 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6661 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6721 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6781 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6841 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6901 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6961 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7021 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7081 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7141 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7201 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7261 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7321 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7381 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7441 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7501 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7561 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7621 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7681 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7741 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7801 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7861 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7921 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7981 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8041 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8101 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8161 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8221 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8281 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8341 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8401 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8461 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8521 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8581 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8641 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8701 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
