LOCUS       sequence-416696-       10161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To monitor the status of NRG1, the plasmid encodes a recombinant 
            NRG1 fused extracellularly to the mScarlet fluorescent protein and 
            intracellularly to the mNeonGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, 
            Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
  TITLE     Low-affinity ligands of the epidermal growth factor receptor are 
            long-range signal transmitters in collective cell migration of 
            epithelial cells.
  JOURNAL   Cell Rep. 2024 Nov 14;43(11):114986. doi: 
            10.1016/j.celrep.2024.114986.
  PUBMED    39546398
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-11-14"; volume: "43(11):114986. doi"; pages: " 
            10.1016/j.celrep.2024.114986"
COMMENT     Sequence Label: pPBbsr2-NRG1-ScNeo
FEATURES             Location/Qualifiers
     source          1..10161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1669
                     /label=chicken beta-actin promoter
     intron          1670..2686
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2610..2632
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2694..2713
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2756..3451
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2889..2907)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3157..3176
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6225..6774
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6369..6386)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6596..6615
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(7245..7262)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7251..7475
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7522..7538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7546..7562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7570..7576,7577..7594,7595..7600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7615..7636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7870..7932
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7973..7993)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7973..7991)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8012..8028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8036..8052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8060..8066,8067..8084,8085..8090))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8105..8126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8243..8260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8414..9002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8494..8513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9173..9964,9965..10033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9796..9815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10034..10138)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggcgcgcgc cagggggggc gggggggggc gaggggcggg gcggggcgag
     1561 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     1621 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     1681 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     1741 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     1801 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     1861 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     1921 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     1981 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     2041 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     2101 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
     2161 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
     2221 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     2281 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     2341 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     2401 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     2461 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     2521 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     2581 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     2641 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     2701 gctggttatt gtgctgtctc atcattttgg caaagaattg ggatccgaat tcaccatggt
     2761 gagcaagggc gaggcagtga tcaaggagtt catgcggttc aaggtgcaca tggagggctc
     2821 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2881 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct
     2941 gtcccctcag ttcatgtacg gctccagggc cttcaccaag caccccgccg acatccccga
     3001 ctactataag cagtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3061 cggcggcgcc gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa
     3121 ggtgaagctc cgcggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat
     3181 gggctgggaa gcgtccaccg agcggttgta ccccgaggac ggcgtgctga agggcgacat
     3241 taagatggcc ctgcgcctga aggacggagg ccgctacctg gcggacttca agaccaccta
     3301 caaggccaag aagcccgtgc agatgcccgg cgcctacaac gtcgaccgca agttggacat
     3361 cacctcccac aacgaggact acaccgtggt ggaacagtac gaacgctccg agggccgcca
     3421 ctccaccggc ggcatggacg agctgtacaa gggaggctcg agcatgagcg agcgcaaaga
     3481 aggcagaggc aaaggcaagg gaaagaagaa agagcgcggc agcggcaaga agcctgaatc
     3541 tgctgctggc agccagtctc ctgctctgcc tcctaggctg aaagagatga agtcccaaga
     3601 gagcgccgct ggctctaagc tggtgctgag atgcgaaacc agcagcgagt acagcagcct
     3661 gaggttcaag tggttcaaga acggcaacga gctgaaccgg aagaacaagc cccagaacat
     3721 caagatccag aagaagcccg gcaagagcga gctgaggatc aacaaagcca gcctggccga
     3781 ctccggcgag tacatgtgca aagtgatcag caagctgggc aacgacagcg ccagcgccaa
     3841 tatcaccatc gtggaaagca acgagatcat caccggcatg cctgcctcta ccgaaggcgc
     3901 ctatgtgtct agcgagagcc ccatcagaat cagcgtgtcc acagagggcg ccaacaccag
     3961 cagctctacc agcacatcta ccaccggcac cagccacctg gtcaagtgcg ccgagaaaga
     4021 aaagaccttc tgcgtgaatg gcggcgagtg cttcatggtc aaggacctga gcaaccccag
     4081 cagatacctg tgcaagtgcc ccaacgagtt caccggcgac aggtgccaga attacgtgat
     4141 ggccagcttc tacaagcacc tgggcatcga gttcatggaa gccgaggaac tgtaccagaa
     4201 aagggtgctg accatcaccg gaatctgtat cgccctgctg gtcgtgggca ttatgtgcgt
     4261 ggtggcctac tgcaagacca agaagcagag gaagaagctg cacgacaggc tgcggcagtc
     4321 tctgagatcc gagaggaaca acatgatgaa tatcgccaac ggaccccacc atcctaatcc
     4381 tccacctgag aacgtgcagc tggtcaacca gtacgtgtcc aagaacgtga tcagctccga
     4441 gcacatcgtc gagagagagg ccgagacaag cttcagcacc tctcactaca ccagcacagc
     4501 ccaccacagc accaccgtga cacagacccc aagccacagc tggtctaatg gccacaccga
     4561 gagcatcctg agcgagtccc atagcgtgat cgtgatgagc agcgtggaaa acagcaggca
     4621 cagcagccct acaggcggac ctagaggcag actgaatgga accggcggac ccagagagtg
     4681 caacagcttt ctgaggcacg ccagagagac acccgacagc tacagagaca gccctcacag
     4741 cgagagatac gtgtccgcca tgacaacccc tgccagaatg agccccgtgg actttcacac
     4801 ccctagcagc ccaaaaagcc ctcctagcga gatgagccct cctgtgtcca gcatgaccgt
     4861 gtccatgcct agcatggctg tgtccccgtt tatggaagag gaaaggcctc tgctgctcgt
     4921 gacccctcca agactgagag agaagaagtt cgaccaccat ccacagcagt tcagcagctt
     4981 ccatcacaac cccgctcacg acagcaacag cctgcctgct tctccactga ggatcgtcga
     5041 ggacgaggaa tacgagacaa cccaagagta cgagcccgct caagagcccg tgaagaaact
     5101 ggccaatagc aggcgggcca agaggaccaa gcctaacggc cacattgcca acaggctgga
     5161 agtggactcc aacacctcta gccagagcag caactccgag agcgagacag aggatgagag
     5221 agtgggcgaa gataccccat tcctgggcat tcagaatcct ctggccgcct ctctggaagc
     5281 cacaccagct tttagactgg ccgacagcag gaccaatcca gccggaagat tcagcaccca
     5341 agaggaaatc caggccagac tgtcctccgt gatcgccaat caggatccta tcgccgtggg
     5401 cggattcgaa atggtaagca agggggagga agataatatg gcttcccttc cagcaacgca
     5461 cgaattgcat atctttggaa gtattaatgg agttgatttc gacatggtgg ggcagggtac
     5521 gggaaaccca aacgatggct atgaagaact taacctcaag tctaccaaag gcgatctcca
     5581 attctcaccc tggatattgg tcccacacat cggctacggg ttccatcaat acctgcctta
     5641 tcctgacggg atgagtcctt ttcaagctgc tatggttgac ggaagtggct accaagttca
     5701 ccgcacaatg caatttgagg atggtgcatc tcttacggtt aattaccgat atacatatga
     5761 gggttcacac atcaaggggg aagctcaggt caagggcact ggatttcccg cagatggccc
     5821 agttatgacc aattctctga ccgcagccga ttggtgtcga agcaagaaga cctaccctaa
     5881 cgacaagact atcatctcaa ccttcaaatg gtcctacacc acaggtaacg gaaagcggta
     5941 taggtctaca gctcggacaa catatacctt tgcgaagcct atggccgcta actatttgaa
     6001 aaaccaaccg atgtatgttt ttcggaagac cgaattgaaa cattcaaaaa cggagttgaa
     6061 tttcaaagag tggcagaagg cattcaccga cgtaatgggt atggatgagt tgtataaata
     6121 agcggcggcc gctctagagt cgacgggccg cggtaacaat tgttaactaa cttaagctag
     6181 caacggtttc cctctagcgg gatcaattcc gccccccccc cctaacgtta ctggccgaag
     6241 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6301 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6361 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6421 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6481 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     6541 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6601 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6661 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6721 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg
     6781 tcatgaaaac atttaacatt tctcaacaag atctagaatt agtagaagta gcgacagaga
     6841 agattacaat gctttatgag gataataaac atcatgtggg agcggcaatt cgtacgaaaa
     6901 caggagaaat catttcggca gtacatattg aagcgtatat aggacgagta actgtttgtg
     6961 cagaagccat tgcgattggt agtgcagttt cgaatggaca aaaggatttt gacacgattg
     7021 tagctgttag acacccttat tctgacgaag tagatagaag tattcgagtg gtaagtcctt
     7081 gtggtatgtg tagggagttg atttcagact atgcaccaga ttgttttgtg ttaatagaaa
     7141 tgaatggcaa gttagtcaaa actacgattg aagaactcat tccactcaaa tatacccgaa
     7201 attaaaagtc gagatgcatc gatgatctag agctcgctga tcagcctcga ctgtgccttc
     7261 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7321 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     7381 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     7441 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaacctgcag
     7501 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7561 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7621 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7681 gtgccagcgg atcttaaaag ttttgttact ttatagaaga aattttgagt ttttgttttt
     7741 ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt
     7801 gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc
     7861 gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt
     7921 atctttctag ggttaatcta gtatacgcgt ttaattaatc cagcttttgt tccctttagt
     7981 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8041 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8101 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8161 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8221 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8281 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8341 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8401 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8461 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8521 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8581 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8641 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8701 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8761 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8821 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8881 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8941 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9001 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9061 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9121 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9181 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9241 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9301 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9361 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9421 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9481 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9541 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9601 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9661 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9721 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9781 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9841 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9901 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9961 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10021 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10081 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10141 catttccccg aaaagtgcca c
//
