LOCUS       sequence-416703-       13509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13509)
  AUTHORS   Whitford CM, Gockel P, Faurdal D, Gren T, Sigrist R, Weber T
  TITLE     CASCADE-Cas3 enables highly efficient genome engineering in 
            Streptomyces species.
  JOURNAL   Nucleic Acids Res. 2025 Mar 20;53(6):gkaf214. doi: 
            10.1093/nar/gkaf214.
  PUBMED    40138716
REFERENCE   2  (bases 1 to 13509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-03-20"; volume: "53(6):gkaf214. doi"; 
            pages: " 10.1093/nar/gkaf214"
COMMENT     Sequence Label: pCRISPR-Cas3
FEATURES             Location/Qualifiers
     source          1..13509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            466..575
                     /note="incP origin of transfer"
     CDS             891..1691
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDSFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELLAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      1993..2580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2481..2500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2734..2751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2868..2889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2904..2909,2910..2927,2928..2934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2942..2958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2947..2969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2966..2982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2966..2982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(3342..3376)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     mobile_element  9348..10115
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     terminator      complement(10115..10153)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             11871..13313
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGQHPGDCLRVDTAVA"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgacccgt gccgggccgc agcggatcgc ggcagcagga gctccccgcc
      181 cgccacggct ggctggatgg tcgcctcacg cgcccgcagg ggtgacggcg tgggtgcggc
      241 ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga ccgcgaccac ccctcccttc
      301 ccgcctgccg tctttcaccg cccggccggt ccgcagacgc cgtacggcca ggccacggag
      361 gattacgggc agatggcgcc cacccggcac ccgggctggg gcaccctgga cagcacgaag
      421 gtccgcccca ctcaccgggg cgggccttcg cgttgagcta gagttcccgc cagcctcgca
      481 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
      541 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
      601 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
      661 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaat gcagctcacg
      721 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
      781 atgccggcct ttgaatgggt tcatgtgcag ctccataagc aaaaggggat gataagttta
      841 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
      901 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
      961 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     1021 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctgcg ctgggtccgg
     1081 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     1141 cgtcgcccgt tacaccggac cttggagttg tctctgactc attctggcgc ctgccaaatg
     1201 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     1261 tctctgatcc attgcccctg ccacctcact cgcctgcaag cccggtcgcc cgtgtccatg
     1321 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     1381 ttgccgagtt gttggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     1441 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     1501 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     1561 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     1621 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     1681 agtcgattgg ctgagctcat aagttcctat tccgaagttc ctattcgaaa tgaccgacca
     1741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1861 gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat
     1921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt cgtgcacaca gcccagcttg
     2341 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2401 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2461 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2521 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2581 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2641 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2701 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2761 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2821 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     2881 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     2941 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaattgtacg
     3001 cggtcgatct tgacggctgg cgagaggtgc ggggaggatc tgaccgacgc ggtccacacg
     3061 tggcaccgcg atgctgttgt gggcacaatc gtgccggttg gtaggatcga cgggtcgcgc
     3121 cccgcacggg cgcgtggatt gaaaccatgg tcgcccggca aaaccgggcg tggattgaaa
     3181 cgtaggcgtc agctacgatg ttccggggac tgctgatccg gtcagcaggt ggaagaggga
     3241 ctggattcca aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg ttgacgacga
     3301 catggctcga ttggcgcgac aagttgctgc gattctcacc aataaaaaac gcccggcggc
     3361 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga ccggatcggg
     3421 gatctgggct gagggagccg acggcacgcg gcggctcacg gcgtggcacg cggaacgtcc
     3481 gggcttgcac ctcacgtcac gtgaggaggc agcgtggacg gcgtcagaga agggagcgga
     3541 catatggacg ccgaggcgtc cgacacccac ttcttcgccc actccaccct gaaggccgac
     3601 cggagcgact ggcagcccct ggtggagcac ctgcaggccg tggcgcgcct ggccggcgag
     3661 aaggccgcgt tcttcggcgg cggcgagctg gccgcgctgg ccggcctgct gcacgacctg
     3721 ggcaagtaca ccgacgagtt ccagcgtcgc atcgccggcg acgcgatccg ggtcgaccac
     3781 tccacccgcg gcgccatcct ggcggtggag cggtacggcg ccctgggcca gctgctggcc
     3841 tacggcatcg cgggccacca cgccggcctg gcgaacggcc gggaggccgg cgagcgcacc
     3901 gcgctggtcg accggctgaa gggcgtgggc ctgccccgcc tgctggaggg ctggtgcgtg
     3961 gagatcgtgc tgcccgagcg cctgcagccg cccccgctga aggcccggct ggagcggggc
     4021 ttcttccagc tggccttcct gggccggatg ctgttctcgt gcctggtcga cgccgactac
     4081 ctggacaccg aggccttcta ccaccgggtg gagggccgtc gcagcctgcg ggagcaggcc
     4141 cgccccaccc tggcggagct gcgggccgcg ctggaccgcc acctgaccga gttcaagggc
     4201 gacaccccgg tcaaccgcgt gcggggcgag atcctggccg gcgtccgcgg caaggcgtcc
     4261 gagctgccgg gcctgttctc gctgaccgtg cccaccggcg gcggcaagac cctggccagc
     4321 ctggccttcg ccctggacca cgccctggcg cacggcctgc gtcgcgtcat ctacgtgatc
     4381 cccttcacct ccatcgtcga gcagaacgcc gccgtgttcc gtcgcgccct gggcgcgctg
     4441 ggcgaggaag ccgtcctgga gcaccacagc gccttcgtgg acgaccgtcg ccagtccctg
     4501 gaggccaaga agaagctgaa cctggcgatg gagaactggg acgcccccat cgtggtgacc
     4561 accgccgtcc agttcttcga gagcctgttc gccgaccggc ccgcccagtg ccgcaagctg
     4621 cacaacatcg ccggctccgt ggtgatcctg gacgaggccc agaccctgcc cctgaagctg
     4681 ctgcggccct gcgtggccgc cctggacgag ctggccctga actaccgctg ctccccggtg
     4741 ctgtgcaccg ccacccagcc ggccctgcag tcccccgact tcatcggcgg cctgcaggac
     4801 gtccgggagc tggccccgga gccccagcgg ctgttccgcg agctggtccg cgtgcgcatc
     4861 cgcaccctgg gccccctgga ggacgccgcc ctgaccgagc agatcgcccg tcgcgagcag
     4921 gtcctgtgca tcgtgaacaa ccgtcgccag gcccgtgccc tgtacgagtc cctggccgag
     4981 ctgcccggcg cccgccacct gaccaccctg atgtgcgcca agcaccggtc ctcggtcctg
     5041 gcggaggtgc gccagatgct gaagaagggc gagccctgcc gcctggtggc cacctccctg
     5101 atcgaggccg gcgtggacgt ggacttcccc gtggtgctgc gggccgaggc cggcctggac
     5161 tcgatcgccc aggccgcggg ccggtgcaac cgcgagggca agcgccccct ggccgagtcc
     5221 gaggtcctgg tgttcgccgc ggccaacagc gactgggcac cgccggagga gctgaagcag
     5281 ttcgcccagg ccgcccggga ggtcatgcgc ctgcaccccg acgactgcct gtcgatggcg
     5341 gccatcgagc ggtacttccg catcctgtac tggcagaagg gcgccgagga gctggacgcc
     5401 ggcaacctgc tgggcctgat cgagcgcggt cgcctggacg gcctgcccta cgagaccctg
     5461 gccaccaagt tccgcatgat cgactccctg cagctgcccg tcatcatccc gttcgacgac
     5521 gaggcccgtg ccgccctgcg cgagctggag ttcgccgacg gctgcgccgc catcgcccgt
     5581 cgcctgcagc cctacctggt ccagatgccg cggaagggct accaggccct gcgggaggcc
     5641 ggcgccatcc aggccgccgc aggcacccgg tacggcgagc agttcatggc cctggtcaac
     5701 cccgacctgt accaccacca gttcggcctg cactgggaca accccgcctt cgtgagctcc
     5761 gagcgcctgt gctggtagtc gggacgcgca acagcggcct ggcctggatg atgtgaaagg
     5821 gagggccgat ggcctacggc atccgcctga tggtctgggg cgagcgggcc tgcttcaccc
     5881 ggcccgagat gaaggtcgag cgcgtgagct acgacgccat caccccctcc gccgcgcggg
     5941 gcatcctgga ggccatccac tggaagcccg ccatccgctg ggtggtggac cgcatccagg
     6001 tcctgaagcc catccgcttc gagtccatcc gtcgcaacga ggtgggcggc aagctgtccg
     6061 ccgtgtccgt gggcaaggcc atgaaggccg gccggaccaa cggcctggtc aacctggtgg
     6121 aggaagaccg gcagcagcgg gccaccaccc tgctgcgcga cgtctcgtac gtgatcgagg
     6181 cgcacttcga gatgaccgac cgggccggcg ccgacgacac cgtcggcaag cacctggaca
     6241 tcttcaaccg tcgcgcccgc aagggccagt gcttccacac cccctgcctg ggcgtccggg
     6301 agttcccggc ctcgttccgg ctgctggagg aaggctccgc cgagccggag gtggacgcgt
     6361 tcctgcgcgg cgagcgggac ctgggctgga tgctgcacga catcgacttc gccgacggca
     6421 tgaccccgca cttcttccgg gccctgatgc gggacggcct gatcgaggtg cccgccttcc
     6481 gggccgcgga ggacaaggca tgatcctgtc cgccctgaac gactactacc agcgcctgct
     6541 ggagcggggc gaggccaaca tctccccctt cggctactcg caggagaaga tcagctacgc
     6601 cctgctgctg tccgcccagg gcgagctgct ggacgtccag gacatccggc tgctgtccgg
     6661 caagaagccc cagccccggc tgatgagcgt cccccagccg gagaagcgca cctccggcat
     6721 caagagcaac gtgctgtggg acaagaccag ctacgtgctg ggcgtgtccg ccaagggcgg
     6781 cgagcggacc cagcaggagc acgagtcctt caagaccctg caccgccaga tcctggtggg
     6841 cgagggcgac ccgggcctgc aggccctgct gcagttcctg gactgctggc agccggagca
     6901 gttcaagccg cccctgttct cggaggccat gctggacagc aacctggtgt tccggctgga
     6961 cggccagcag cggtacctgc acgagacccc cgccgcgctg gccctgcgca cccggctgct
     7021 ggcggacggc gactcccgcg agggcctgtg cctggtctgc ggccagcgcc agccgctggc
     7081 ccggctgcac cccgcggtca agggcgtgaa cggcgcccag tcctccggcg cgtccatcgt
     7141 gtcgttcaac ctggacgcct tcagctccta cggcaagagc cagggcgaga acgcccccgt
     7201 ctccgagcag gccgccttcg cctacaccac cgtgctgaac cacctgctgc gtcgcgacga
     7261 gcacaaccgg cagcggctgc agatcggcga cgcctccgtg gtgttctggg cccaggccga
     7321 caccccggcc caggtggccg ccgcagagtc gaccttctgg aacctgctgg agcctccggc
     7381 cgacgacggc caggaagccg agaagctgcg gggcgtcctg gacgccgtgg ccaccggccg
     7441 cccgctgcac gagctggact cgctgatgga ggaaggcacc cgcatcttcg tgctgggcct
     7501 ggcccccaac accagccgcc tgtccatccg cttctgggcc gtggacagcc tggcggtgtt
     7561 cacccagcac ctggccgagc acttccgcga catgcacctg gagcccctgc cctggaagac
     7621 cgagcccgcc atctggcgcc tgctgtacgc caccgccccc tcccgcgacg gccgggccaa
     7681 gaccgaggac ctgctgcccc agctggccgg cgagatgacc cgggcgatcc tgaccggctc
     7741 ccgctacccc cggtccctgc tggccaacct gatcatgcgg atgcgggccg acggcgacgt
     7801 cagcggcatc cgcgtggccc tgtgcaaggc cgtcctggcc cgcgaggccc ggctgtccgg
     7861 caagatccac caggaagagc tgccgatgtc cctggacaag gacgcctcga accccggcta
     7921 ccgcctgggc cggctgttcg ccgtcctgga gggcgcccag cgggccgccc tgggcgaccg
     7981 ggtgaacgcc accatccgcg accggtacta cggcgccgcc tcctccaccc ccgcgaccgt
     8041 cttccccatc ctgctgcgga acacccagaa ccacctggcc aagctgcgca aggagaagcc
     8101 cggcctggcg gtgaacctgg agcgggacat cggcgagatc atcgacggca tgcagtcgca
     8161 gttcccgcgc tgcctgcggc tggaggacca gggccgcttc gccatcggct actaccagca
     8221 ggcgcaggcc cgcttcaacc ggggcccgga ctccgtcgag taaggagcag aagaatgacc
     8281 gccatcagca accgctacga gttcgtctac ctgttcgacg tgtccaacgg caaccccaac
     8341 ggcgaccccg acgccggcaa catgccccgg ctggaccccg agaccaacca gggcctggtc
     8401 accgacgtgt gcctgaagcg caagatccgg aactacgtct ccctggagca ggagtccgcc
     8461 cccggctacg cgatctacat gcaggagaag tcggtgctga acaaccagca caagcaggcc
     8521 tacgaggcgc tgggcatcga gagcgaggcc aagaagctgc ccaaggacga ggccaaggcg
     8581 cgcgagctga cctcctggat gtgcaagaac ttcttcgacg tccgggcctt cggcgccgtg
     8641 atgaccaccg agatcaacgc cggccaggtc cggggcccca tccagctggc cttcgcgacc
     8701 agcatcgacc cggtcctgcc gatggaggtg tccatcaccc ggatggccgt caccaacgag
     8761 aaggacctgg agaaggagcg cacgatgggc cggaagcaca tcgtgcccta cggcctgtac
     8821 cgggcccacg gcttcatctc cgccaagctg gcggagcgga ccggcttcag cgacgacgac
     8881 ctggagctgc tgtggcgggc cctggcgaac atgttcgagc acgaccggtc cgccgcgcgg
     8941 ggcgagatgg ccgcgcggaa gctgatcgtc ttcaagcacg agcacgcgat gggcaacgcc
     9001 cccgcccacg tgctgttcgg ctccgtcaag gtggagcggg tggagggcga cgccgtgacc
     9061 cccgcccgcg gtttccagga ctaccgggtc tccatcgacg ccgaggcgct gccccagggc
     9121 gtctcggtgc gcgagtacct gtagaagctt tctagaggat ccccgggtac cgagctcgaa
     9181 ttccccagat ctaaagtttt gtcgtctttc cagacgttag taaatgaatt ttctgtatga
     9241 ggttttgcta aacaactttc aacagtttca gcggagtgag aatagaaagg aacaactaaa
     9301 ggaattgcga ataataattt tttcacgttg aaaatctcca aaaaaaaggt aatgactcca
     9361 acttattgat agtgttttat gttcagataa tgcccgatga ctttgtcatg cagctccacc
     9421 gattttgaga acgacagcga cttccgtccc agccgtgcca ggtgctgcct cagattcagg
     9481 ttatgccgct caattcgctg cgtatatcgc ttgctgatta cgtgcagctt tcccttcagg
     9541 cgggattcat acagcggcca gccatccgtc atccatatca ccacgtcaaa gggtgacagc
     9601 aggctcataa gacgccccag cgtcgccata gtgcgttcac cgaatacgtg cgcaacaacc
     9661 gtcttccgga gactgtcata cgcgtaaaac agccagcgct ggcgcgattt agccccgaca
     9721 tagccccact gttcgtccat ttccgcgcag acgatgacgt cactgcccgg ctgtatgcgc
     9781 gaggttaccg actgcggcct gagtttttta agtgacgtaa aatcgtgttg aggccaacgc
     9841 ccataatgcg ggctgttgcc cggcatccaa cgccattcat ggccatatca atgattttct
     9901 ggtgcgtacc gggttgagaa gcggtgtaag tgaactgcag ttgccatgtt ttacggcagt
     9961 gagagcagag atagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag
    10021 tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc
    10081 atcatacact aaatcagtaa gttggcagca tcacccaaaa aaaaaggctc caaaaggagc
    10141 ctttaattgt atcggtttat cagcttgctt tcgaggtgaa tttcttaaac agcttgatac
    10201 cgatagttgc gccgacaatg acaacaacca tcgcccacgc ataaccgata tattcggtcg
    10261 ctgaggcttg cagggagtca aaggccgctt ttgcgggatc tcgtcgaagg cactagaggc
    10321 ctatatgcgg ggatcgaccg cgcgggtccc ggacggggaa gagcggggag ctttgccaga
    10381 gagcgacgac ttccccttgc gttggtgatt gccggtcagg gcagccatcc gccatcgtcg
    10441 cgtagggtgt cacaccccag gaatcgcgtc actgaacaca gcagccggta ggacgaccat
    10501 gactgagttg gacaccatcg caaatccgtc cgatcccgcg gtgcagcgga tcatcgatgt
    10561 caccaagccg tcgcgatcca acataaagac aacgttgatc gaggacgtcg agcccctcat
    10621 gcacagcatc gcggccgggg tggagttcat cgaggtctac ggcagcgaca gcagtccttt
    10681 tccatctgag ttgctggatc tgtgcgggcg gcagaacata ccggtccgcc tcatcgactc
    10741 ctcgatcgtc aaccagttgt tcaaggggga gcggaaggcc aagacattcg gcatcgcccg
    10801 cgtccctcgc ccggccaggt tcggcgatat cgcgagccgg cgtggggacg tcgtcgttct
    10861 cgacggggtg aagatcgtcg ggaacatcgg cgcgatagta cgcacgtcgc tcgcgctcgg
    10921 agcgtcgggg atcatcctgg tcgacagtga catcaccagc atcgcggacc ggcgtctcca
    10981 aagggccagc cgaggttacg tcttctccct tcccgtcgtt ctctccggtc gcgaggaggc
    11041 catcgccttc attcgggaca gcggtatgca gctgatgacg ctcaaggcgg atggcgacat
    11101 ttccgtgaag gaactcgggg acaatccgga tcggctggcc ttgctgttcg gcagcgaaaa
    11161 gggtgggcct tccgacctgt tcgaggaggc gtcttccgcc tcggtttcca tccccatgat
    11221 gagccagacc gagtctctca acgtttccgt ttccctcgga atcgcgctgc acgagaggat
    11281 cgacaggaat ctcgcggcca accgataagc gcctctgttc ctcggacgct cggttgctcg
    11341 acctcgattc gtcagtgatg atcaacagga cccagaaatg gcacgagccc gggattggcc
    11401 tcccgggctc gccgcccccg aaccggcgca gcatgccgag tgaggacgtt tgctctgtga
    11461 cgagcgtacg agagaacagc gcgtcgacac caccgcgccc cgccggaccg aagtgccact
    11521 gcggcacccc gatcgtccac atccccggga agcgccccaa aatctactgc tcggacgcct
    11581 gccgaaagcg cgcaaagcgt ctcattgcgg accagtcacg cagcgtagca agcacgacta
    11641 cagcgggtaa tccggacgcg ggctttagta gtaagggaaa atctccgtcc actgcaggtc
    11701 agagcgctgt aactggaacg ggttctaccc caaacgaggg tgccgcgaca gccgggcggg
    11761 aggacccgcg cgacgagcgg ttcgcccggc gggaccggca ccagacggtg tcgctgaacg
    11821 aggcgttcaa gggctgcggg acccggctca ctgccgggac ggccgagctg atgtggaagc
    11881 cgggcgaggc gacgtggggc aacacgtgcc gctgcaacaa cgtccacacc tgcccgtggt
    11941 gcatgagccg catcctggcc gtacggggct cgaacgtgca gctcgccgcc gacggtctcg
    12001 cggacgccgg gtacgggctg caccttggca cgaacacgtt gcgccacttc gagcgcatgg
    12061 cgttcggcac cgtccgcaag ggaatgcggc acggcctggt cgcggtcttg cacgacggct
    12121 ggaagggcgc gtacggctcg tccggccgcc gctggcgcac gatgcgcgac gacttcggga
    12181 tcatcggcta cgagcgggcc ttcgaggaca ccttcggctg gggctccggc tggcacctgc
    12241 actggcacac gctctgggtc acccgcgagg tgctcggccc ggacgcccag gccgcgttcc
    12301 gcgacgcact cgccggagcc tgggccgccg gggtcgaatc cgcgggcggc tacaccgtca
    12361 gcgagacgtg cgaccggccc ggttgctcct gtgagggcaa gggccacggc accgacgtgc
    12421 gcccactcaa cggcgcggac gccgctgacg gcgatgcggg caagcaggcc cgctacctct
    12481 acaaggacgg cgacaagacc aagggcggcg tcgccaagat cggcctggag ctggccggac
    12541 agaacttcaa ggccggtcgc ggcgacgacc gcatgggacc gctcgacctg ggcgacgcgg
    12601 cggccgccga gctgcagcgg cttcgtcggc ccggcccgtt cgtcgagaag taccgcgagc
    12661 gcgagttcgg cgtcttccag gtccgcaagc actaccggtc gcagaacctg aaccggctga
    12721 tcaaggaact cggcatccag caggacgtcc gcaccgagga ggagatcacc gacgacaccg
    12781 agggcctggt cgccatcgcg gtcatcccgg cctacatctg gtaccgatac atcgcccgcg
    12841 tcgccggacg tcgcctcgac ctcatcaagg tcgccgagac gtacggccta ccgggcgtcc
    12901 ggcggctcgt cgagtcctgg ggcctggtgt ggggcaagga cgtcctggac ccgcccgccc
    12961 cggaagcgcc ggccgcgccg ggcgaccttg acgctgacca gatgaggttc gaggtcatgt
    13021 ccgaggagga agctgctttc cgcgaggcgc gccggaaggc caacgaggcc cgcacggagg
    13081 agctggccgc ttcgctcgac cgagtgcgtc agccgaagaa ggaggcgatc cgcccgacga
    13141 tcagccttcg gaagcgcctg aagccgaagc cggtcaccgt cgacgtcaag actcccccgc
    13201 ccggcgccgc gtcgcccgtg tgccgccgct gcaagggcaa gctcgccccg gtactccagc
    13261 cctggggcca gcaccccgga gactgcctcc gcgtcgacac agcggtcgcg tagggccgct
    13321 gagggaccgc catgagaccg ccaccccgga catccgggtg gcggtctcat ggcggtcccg
    13381 gcgcgaaagc cgagggtctg cctgccgtga ggtggccggc ggacctgggt tactgctgga
    13441 atcccgcgcc cagtccgagc gtccgccggc gcttttcacc tggctccggg tgctcgtcga
    13501 tgagcacca
//
