LOCUS       sequence-416731-        1698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the affinity sorting tag GST-EGFP- GPIDAF..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma S, Yang L, Zuo Q, Huang Q
  TITLE     GPI-anchored glutathione S-transferase as marker allows affinity 
            sorting of transfection-positive cells.
  JOURNAL   Front Mol Biosci. 2022 Sep 29;9:1016090. doi: 
            10.3389/fmolb.2022.1016090. eCollection 2022.
  PUBMED    36250010
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Biosci."; date: "2022-09-29"; volume: "9:1016090. doi"; pages: " 
            10.3389/fmolb.2022.1016090. eCollection 2022"
COMMENT     Sequence Label: pGST-EGFP-GPIDAF
FEATURES             Location/Qualifiers
     source          1..1698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             124..780
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     738..760
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             787..804
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      811..820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(817..819,820..822,823..1533)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(817..819,820..822,823..1533)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(862..883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1123..1142)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1470..1491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgaccgtgg ctagacccag cgtgcctgct gccctgcctc tgctgggaga gctgcctaga
       61 ctgctgctcc tggtgctcct gtgcctgcct gctgtgtggg gaagcgcctg gagccaccct
      121 cagatgggct cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
      181 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
      241 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
      301 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
      361 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
      421 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
      481 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
      541 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
      601 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
      661 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
      721 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
      781 tcggatctgg ttccgcgtgg atccccggtc gccaccatgg tgagcaaggg cgaggagctg
      841 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      901 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      961 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1021 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1081 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1141 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1201 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1261 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1321 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1381 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1441 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1501 gggatcactc tcggcatgga cgagctgtac aagtccggcc ggactcagat cagccggacc
     1561 accaagcact tccacgagac cacccctaac aagggcagcg gcaccaccag cggcaccacc
     1621 cggctgctga gcggccacac ctgcttcacc ctgaccggcc tgctgggcac cctggtgacc
     1681 atgggcctgc tgacctag
//
