LOCUS       sequence-416733-        1635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the affinity sorting tag GST-EGFP- GPIBY55..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma S, Yang L, Zuo Q, Huang Q
  TITLE     GPI-anchored glutathione S-transferase as marker allows affinity 
            sorting of transfection-positive cells.
  JOURNAL   Front Mol Biosci. 2022 Sep 29;9:1016090. doi: 
            10.3389/fmolb.2022.1016090. eCollection 2022.
  PUBMED    36250010
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Biosci."; date: "2022-09-29"; volume: "9:1016090. doi"; pages: " 
            10.3389/fmolb.2022.1016090. eCollection 2022"
COMMENT     Sequence Label: pGST-EGFP-GPIBY55
FEATURES             Location/Qualifiers
     source          1..1635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..753
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     711..733
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             760..777
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(790..792,793..795,796..1506)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(790..792,793..795,796..1506)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(835..856)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1096..1115)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1443..1464
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgctgctgg agcctggcag aggctgctgc gctctggcca tcctgctggc catcgtggac
       61 atccagagcg gaggcagcgc ctggagccac cctcagatgg gctcccctat actaggttat
      121 tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa
      181 tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa
      241 ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag
      301 tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa
      361 gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg
      421 agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct
      481 gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat
      541 gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca
      601 atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca
      661 caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa
      721 gccacgtttg gtggtggcga ccatcctcca aaatcggatc tggttccgcg tggatccccg
      781 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      841 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      901 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      961 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1021 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1081 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1141 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1201 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1261 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1321 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1381 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1501 tacaagtccg gccggactca gatccggcag cacctggagt tcagccacaa cgagggcacc
     1561 ctgagcagcg gcttcctcca ggagaaggtg tgggtgatgc tggtgaccag cctggtggct
     1621 ctccaggccc tgtag
//
