LOCUS       sequence-416735-        1683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the affinity sorting tag GST-EGFP- GPICEAM7..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma S, Yang L, Zuo Q, Huang Q
  TITLE     GPI-anchored glutathione S-transferase as marker allows affinity 
            sorting of transfection-positive cells.
  JOURNAL   Front Mol Biosci. 2022 Sep 29;9:1016090. doi: 
            10.3389/fmolb.2022.1016090. eCollection 2022.
  PUBMED    36250010
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Biosci."; date: "2022-09-29"; volume: "9:1016090. doi"; pages: " 
            10.3389/fmolb.2022.1016090. eCollection 2022"
COMMENT     Sequence Label: pGST-EGFP-GPICEAM7
FEATURES             Location/Qualifiers
     source          1..1683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             130..786
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     744..766
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             793..810
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(823..825,826..828,829..1539)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(823..825,826..828,829..1539)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(868..889)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1129..1148)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1476..1497
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgggcagcc ctagcgcctg cccttacaga gtgtgcatcc cctggcaggg cctgctgctg
       61 accgccagcc tgctgacctt ctggaacctg cccaacagcg cccagaccag cgcctggagc
      121 caccctcaga tgggctcccc tatactaggt tattggaaaa ttaagggcct tgtgcaaccc
      181 actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta tgagcgcgat
      241 gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc caatcttcct
      301 tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg ttatatagct
      361 gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc aatgcttgaa
      421 ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa agactttgaa
      481 actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt cgaagatcgt
      541 ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt catgttgtat
      601 gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt cccaaaatta
      661 gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt gaaatccagc
      721 aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg cgaccatcct
      781 ccaaaatcgg atctggttcc gcgtggatcc ccggtcgcca ccatggtgag caagggcgag
      841 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      901 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      961 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1021 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1081 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1141 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1201 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1261 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1321 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1381 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1441 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1501 gccgccggga tcactctcgg catggacgag ctgtacaagt ccggccggac tcagatcagc
     1561 cggagcgacc ctgtgaccct gaacgtgaga tacgagagcg tgcaggccag cagccctgac
     1621 ctgagcgccg gcaccgccgt gagcatcatg atcggcgtgc tggctggcat ggctctgatc
     1681 tag
//
